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Covalent grafting tyrosinase and its application in phenolic compounds detection

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Abstract

A stepwise strategy is reported for the design of a meditor-free amperometric tyrosinase biosensor. It is based on the azide-alkyne click reaction and carbodiimide coupling. Firstly, azide-terminated alkane thiols monolayers were self-assembled on the Au electrode surface. Then, nitrophenyl groups were covalent attached to the self-assembled monolayers (SAMs) via the click reaction of copper(I)-catalyzed 1,3-dipolar cycloadditions of azide-alkyne. Finally, the nitrophenyl group terminated SAMs were converted to aminophenyl-terminated interface by electrochemical reduction, and tyrosinase was covalent immobilized onto the Au electrode via carbodiimide reaction. Based on the stepwise strategy, a meditor-free amperometric tyrosinase biosensor was farbricated, and it showed good electrocatalytic reduction ability toward phenol, pyrocatechol and m-Cresol. Their linear ranges were over the range of 0.2 to 15.0 μmol·L−1, 0.2 to 73.0 μmol·L−1, and 0.2 to 33.0 μmol·L−1, respectively.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 20875031), Shanghai Rising-Star Program (09QH1400800), New Century Excellent Talents in University (NCET-09-0357) and Shanghai Municipal Education Commission (No. 08ZZ25).

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai, 200062, China

    Liwei Wang, Qin Ran, Yuan Tian, Siqiu Ye, Jingjing Xu, Yuezhong Xian, Ru Peng & Litong Jin

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  1. Liwei Wang
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  2. Qin Ran
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  3. Yuan Tian
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  4. Siqiu Ye
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Correspondence to Yuezhong Xian.

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Wang, L., Ran, Q., Tian, Y. et al. Covalent grafting tyrosinase and its application in phenolic compounds detection. Microchim Acta 171, 217–223 (2010). https://doi.org/10.1007/s00604-010-0433-y

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  • DOI: https://doi.org/10.1007/s00604-010-0433-y

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