Skip to main content
SpringerLink
Log in

Amperometric tyrosinase biosensor based on boron-doped nanocrystalline diamond film electrode for the detection of phenolic compounds

  • Original Paper
  • Published:
Journal of Solid State Electrochemistry Aims and scope Submit manuscript

Abstract

A novel tyrosinase amperometric biosensor based on boron-doped nanocrystalline diamond (BDND) film electrode has been developed for the detection of phenolic compounds. Amine-terminated BDND surface was achieved via a direct photochemical reaction with allylamine. The tyrosinase was then covalently immobilized on the amine-terminated diamond film electrode surface. The effects of pH and applied potential on the performance of tyrosinase amperometric biosensor were investigated. The biosensor showed an optimum response at a pH of 6.5 and at an applied potential of −100 mV. It also exhibited good analytical performances to phenolic compounds in terms of sensitivity, detection limit, and stability. The tyrosinase-modified BDND film electrode exhibited a rapid response to the changes in the substrate concentration for all the phenolic compounds tested and reached 95 % of steady-state current in about 10 s. It is attributed to the high loading of tyrosinase and the rapid electron transfer between the tyrosinase and BDND film electrode surface. The tyrosinase-modified BDND film electrode showed high sensitivity with 184.0, 95.6, and 552.3 mA M−1 cm−2 and a linear response range of 10.0–120.0, 5.0–120.0, and 30.0–120.0 μM for phenol, catechol, and 4-chlorophenol, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

References

  1. Luthria D, Singh AP, Wilson T, Vorsa N, Banuelos GS, Vinyard BT (2010) Food Chem 121:406–411

    Article  CAS  Google Scholar 

  2. Andrade LS, Rocha-Filho RC, Bocchi N, Biaggio SR, Iniesta J, Garcia-Garcia V, Montiel V (2008) J Hazard Mater 153:252–260

    Article  CAS  Google Scholar 

  3. Yeh YL, Yeh KJ, Hsu LF, Yu WC, Lee MH, Chen TC (2014) J Hazard Mater 277:27–33

    Article  CAS  Google Scholar 

  4. Turkia H, Sirén H, Penttilä M, Pitkänen JP (2013) J Chromatogr A 1278:175–180

    Article  CAS  Google Scholar 

  5. Wang HL, Yan H, Wang CJ, Chen F, Ma MP, Wang WW, Wang XD (2012) J Chromatogr A 1253:16–21

    Article  CAS  Google Scholar 

  6. Jing X, Zhang X, Bao J (2009) Appl Biochem Biotechnol 159:696–707

    Article  CAS  Google Scholar 

  7. Poerschmann J, Zhang ZY, Kopinke FD, Pawliszyn J (1997) Anal Chem 69:597–600

    Article  CAS  Google Scholar 

  8. Omuro Lupetti K, Rocha FRP, Fatibello-Filho O (2004) Talanta 62:463–467

    Article  CAS  Google Scholar 

  9. Kovács A, Mortl M, Kende A (2011) Microchem J 99:125–131

    Article  CAS  Google Scholar 

  10. Şenyurt Ö, Eyidoğan F, Yilmaz R, Tufan Öz M, Cengiz Özalp V, Arica Y, Öktem Hüseyin A (2015) Biotechnol Appl Biochem 62:1332–1136

    Google Scholar 

  11. Wang BN, Zheng JB, He YP, Sheng QL (2013) Sensor Actuators B 186:417–422

    Article  CAS  Google Scholar 

  12. Yang LJ, Xiong HY, Zhang XH, Wang SF (2012) Bioelectrochemistry 84:44–48

    Article  CAS  Google Scholar 

  13. Hashemnia S, Khayatzadeh S, Hashemnia M (2012) J Solid State Electrochem 16:473–479

    Article  CAS  Google Scholar 

  14. Stoytchev M, Zlatev R, Goche V, Velkov Z, Montero G, Beleño MT (2014) Electrochim Acta 147:25–30

    Article  CAS  Google Scholar 

  15. Qu Y, Ma M, Wang ZG, Zhan GQ, Li BH, Wang X, Fang HF, Zhang HJ, Li CY (2013) Biosens Bioelectron 44:85–88

    Article  CAS  Google Scholar 

  16. Han E, Yang Y, He Z, Cai J, Zhang X, Dong X (2015) Anal Biochem 486:102–106

    Article  CAS  Google Scholar 

  17. Apetrei C, Rodriguez-Mendez ML, De Saja JA (2011) Electrochim. Acta 56:8919–8925

    CAS  Google Scholar 

  18. Pérez JPH, López MSP, Lopez-Cabarcos E, Lopez-Ruiz B (2006) Biosens Bioelectron 22:429–439

    Article  CAS  Google Scholar 

  19. Yildiz HB, Castillo J, Gushin DA, Toppare L, Schumann W (2007) Microchim. Acta 159:27–34

    CAS  Google Scholar 

  20. Shiddiky MJA, Torriero AAJ (2011) Biosens Bioelectron 26:1775–1787

    Article  CAS  Google Scholar 

  21. Takashima RW, Kaneto K (2004) Sensor Actuators B 102:271–277

    Article  CAS  Google Scholar 

  22. Karim MN, Lee HJ (2013) Talanta 116:991–999

    Article  CAS  Google Scholar 

  23. Kumar Vashist S, Zheng D, Al-Rubeaan K, Luong JHT, Sheu FS (2011) Biotechnol Adv 29:169–188

    Article  CAS  Google Scholar 

  24. Granero AM, Fernández H, Agostini E, Zón MA (2010) Talanta 83:249–255

    Article  CAS  Google Scholar 

  25. Apetreia C, Rodríguez-Méndezb ML, De Sajac JA (2011) Electrochim Acta 56:8919–8925

    Article  CAS  Google Scholar 

  26. Fan Q, Shan D, Xue H, He Y, Cosnier S (2007) Biosens Bioelectron 22:816–821

    Article  CAS  Google Scholar 

  27. Singh S, Jain DVS, Singla ML (2013) Sensor Actuators B 182:161–169

    Article  CAS  Google Scholar 

  28. Kochana J, Gala A, Parczewski A, Adamski J (2008) Anal Bioanal Chem 391:1275–1281

    Article  CAS  Google Scholar 

  29. Chawla S, Rawal R, Sharma S, Pundir CS (2012) Biochem Eng J 68:76–84

    Article  CAS  Google Scholar 

  30. Arecchi A, Scampicchio M, Drusch S, Mannino S (2010) Anal Chim Acta 659:133–136

    Article  CAS  Google Scholar 

  31. Aihua L, Itaru H, Haoshen Z (2005) Electrochem Commun 7:233–236

    Article  CAS  Google Scholar 

  32. Karim F, Fakhruddin ANM (2012) Rev Environ Sci Biotechnol 11:261–274

    Article  CAS  Google Scholar 

  33. Lu LM, Zhang L, Zhang XB, Huan SY, Shen GL, Yu RQ (2010) Anal Chim Acta 665:146–151

    Article  CAS  Google Scholar 

  34. Zhao JW, Wu DH, Zhi JF (2009) Bioelectrochemistry 75:44–49

    Article  CAS  Google Scholar 

  35. Janegitz Bruno C, Medeiros Roberta A, Rocha-Filho Romeu C (2012) Fatibello-Filho Orlando. Diam Relat Mater 25:128–133

    Article  CAS  Google Scholar 

  36. Zou YS, He LL, Zhang YC, Li ZX, Wang HP, Gu L, Tu CJ, Zeng HB (2013) Mater Chem Phys 141:851–821

    Article  CAS  Google Scholar 

  37. Wang YG, Lau SP, Tay BK, Zhang XH (2002) J Appl Phys 92:7253–7256

    Article  CAS  Google Scholar 

  38. Zhang GJ, Song KS, Nakamura Y, Ueno T, Funatsu T, Ohdomari I, Kawarada H (2006) Langmuir 22:3728–3734

    Article  CAS  Google Scholar 

  39. Torrengo S, Miotello A, Minati L, Bernagozzi I, Ferrari M, Dipalo M, Kohn E, Speranza G (2011) Diam Relat Mater 20:990–994

    Article  CAS  Google Scholar 

  40. Zhuang H, Srikanth Vadali VSS, Jiang X, Luo J, Ihmels H, Aronov I, Wenclawiak BW, Adlung M, Wickleder C (2009) Appl Phys Lett 95:143703

    Article  CAS  Google Scholar 

  41. Zhou YL, Tian RH, Zhi JF (2007) Biosens Bioelectron 22:822–828

    Article  CAS  Google Scholar 

  42. Zhou YL, Zhi JF (2006) Electrochem Commun 8:1811–1816

    Article  CAS  Google Scholar 

  43. Liu Z, Liu B, Kong J, Deng J (2000) Anal Chem 72:4707–4712

    Article  CAS  Google Scholar 

  44. Wang SF, Tan YM, Zhao DM, Liu GD (2008) Biosens Bioelectron 23:1781–1787

    Article  CAS  Google Scholar 

  45. Lu LM, Zhang L, Zhang XB, Huan SY, Shen GL, Yu RQ (2001) Anal Chim Acta 665:146–151

    Article  CAS  Google Scholar 

  46. Rajesh KK (2005) Curr Appl Phys 5:178–183

    Article  Google Scholar 

  47. Wang B, Zhang J, Dong S (2000) Biosens. Bioelectron 15:397–402

    Article  CAS  Google Scholar 

  48. Kim MA, Lee WY (2003) Anal Chim Acta 479:143–150

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Natural Science Foundation of Jiangsu Province of China (BK20141401) and the Fundamental Research Funds for the Central Universities (30920130111019).

Author information

Authors and Affiliations

  1. School of Materials Science and Engineering, Institute of Optoelectronics and Nanomaterials, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China

    Yousheng Zou, Dong Lou, Kang Dou, Linlin He, Yuhang Dong & Shalong Wang

Authors
  1. Yousheng Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dong Lou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kang Dou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Linlin He
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yuhang Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shalong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yousheng Zou.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zou, Y., Lou, D., Dou, K. et al. Amperometric tyrosinase biosensor based on boron-doped nanocrystalline diamond film electrode for the detection of phenolic compounds. J Solid State Electrochem 20, 47–54 (2016). https://doi.org/10.1007/s10008-015-3003-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10008-015-3003-8

Keywords

Search

Navigation