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Mycorrhiza analyses in New Zealand truffières reveal frequent but variable persistence of Tuber melanosporum in co-existence with other truffle species

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Abstract

This study compiles the results from an examination of mycorrhizae on root samples from Tuber melanosporum truffières in New Zealand. Samples were taken over 5 years from 328 trees in 43 truffières established with nursery-inoculated trees. Mycorrhizae were analysed using a combination of morphological and molecular techniques, focusing on the identification of Tuber species. Results show that 49% of the trees, and nearly 90% of the truffières, retained T. melanosporum mycorrhizae up to 21 years after planting. Tuber mycorrhizae with spiky cystidia were found on 26.9% of the tested trees: Tuber brumale (5.5%), Tuber maculatum (10.7%), and unidentified Tuber species (10.7%), and were detected in 67% of the truffières tested. T. brumale was found in 28% and T. maculatum in 35% of the truffières. In 56% of the truffières, T. melanosporum was found to occur with spiky Tuber species. The existence of T. brumale and T. maculatum in the same truffière was recorded only once. Forty-four percent of trees examined had Scleroderma-like (SCL) mycorrhizae and 50% of trees hosted other ectomycorrhizal species (OE). For all categories of mycorrhizal species examined, the variation between truffières was greater than variation within each truffière. Overall results indicate that Corylus avellana tends to be more receptive to mycorrhizae of Tuber species than Quercus robur but is not necessarily more productive. In productive truffières, Q. robur appears to host SCL mycorrhizae more often than C. avellana. This is the first study of its scale to analyse the mycorrhizal species associated with T. melanosporum truffières in the Southern Hemisphere.

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Acknowledgments

We are grateful to many independent New Zealand truffle growers and members of the New Zealand Truffle Association. Without their cooperation, this study would not have been possible. We thank Carolyn Dixon for assistance with root sampling, and Slaven Kljucanin and Sandra Visnovsky for assistance with mycorrhiza analyses. We thank Simon Bulman, Bob Fullerton and two anonymous reviewers for helpful comments on the manuscript. This study was partially supported by the New Zealand government through various research projects (FRST programme ‘Made in the Shade’ and a TechNZ project).

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  1. Nicholas Cummings

    Present address: Bio-Protection Research Centre, Lincoln University, Lincoln, 7647, New Zealand

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  1. The New Zealand Institute for Plant & Food Research Limited, Gerald Street, Lincoln, Private Bag 4704, Christchurch, 8140, New Zealand

    Alexis Guerin-Laguette, Nicholas Cummings, Nina Hesom-Williams, Ruth Butler & Yun Wang

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  1. Alexis Guerin-Laguette
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Guerin-Laguette, A., Cummings, N., Hesom-Williams, N. et al. Mycorrhiza analyses in New Zealand truffières reveal frequent but variable persistence of Tuber melanosporum in co-existence with other truffle species. Mycorrhiza 23, 87–98 (2013). https://doi.org/10.1007/s00572-012-0450-2

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