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Ectomycorrhizal species associated with Pinus radiata in New Zealand including novel associations determined by molecular analysis

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Abstract

Ectomycorrhizal (ECM) associates of the exotic plantation species Pinus radiata were investigated above and below ground over two years in the North Island of New Zealand. ECM species were identified using morphological and molecular (restriction fragment length polymorphism and DNA sequencing) analysis. Eighteen ECM species were observed fruiting above ground; 19 ECM species were identified below ground. In the above ground study, Wilcoxina mikolae, Rhizopogon pseudoroseolus and Inocybe sindonia were noted for the first time as ECM associates of P. radiata in New Zealand. Below ground, the species W. mikolae, R. pseudoroseolus, Rhizopogon luteorubescens, Pseudotomentella sp., Pseudotomentella tristis and Tomentella sp. were found as new associates of P. radiata in New Zealand. Additionally, six ECM types were found that could not be identified with molecular analysis. The putative ECM taxa Tricholoma pessundatum, Laccaria laccata and Hebeloma crustuliniforme were examined by molecular analysis, and species identifications were proposed to be changed to Tricholoma sp., L. laccata and Hebeloma sp. for specimens associated with P. radiata in New Zealand. The species identity of I. sindonia, previously unidentified to species level, was determined with direct sequencing.

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Acknowledgment

We would like to acknowledge funding from the New Zealand Foundation for Research and Technology (Contract C04X0302). We thank Kaingaroa Timberlands for use of their nursery and plantation sites and Timberlands Ltd. staff for assistance in the field. Ian Hood, Geoff Ridley and Margaret Dick provided assistance in identifying sporocarps and the suggestions of John Bain on the manuscript are greatly appreciated.

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Authors and Affiliations

  1. Bio-Protection Research Centre, PO Box 84, Lincoln University, Lincoln, 7647, New Zealand

    Katrin Walbert

  2. Forest Protection, Scion, 49 Sala Street, Rotorua, 3049, New Zealand

    Katrin Walbert & Tod D. Ramsfield

  3. Department of Ecology, Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Lincoln, 7647, Canterbury, New Zealand

    Hayley J. Ridgway & E. Eirian Jones

Authors
  1. Katrin Walbert
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  2. Tod D. Ramsfield
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  3. Hayley J. Ridgway
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  4. E. Eirian Jones
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Correspondence to Katrin Walbert.

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Scion is the trading name of the New Zealand Forest Research Institute Ltd.

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Walbert, K., Ramsfield, T.D., Ridgway, H.J. et al. Ectomycorrhizal species associated with Pinus radiata in New Zealand including novel associations determined by molecular analysis. Mycorrhiza 20, 209–215 (2010). https://doi.org/10.1007/s00572-009-0277-7

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  • DOI: https://doi.org/10.1007/s00572-009-0277-7

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