Abstract
We explored the diversity of mycorrhizal fungi associated with Monotropastrum humile in the central part of Japan's main island. We collected 103 M. humile individuals from 12 sites with various forest types. We analyzed the DNA sequences of the internal transcribed spacer region from fungal and plant nuclear ribosomal DNAs to assess the genetic diversity of the fungi associated with M. humile roots and to position the plant with respect to known Monotropoideae groups, respectively. The plants formed a monophyletic clade with other members of M. humile but were separated from M. humile var. glaberrimum and other monotropes (97% bootstrap support). Of the 50 fungal phylotypes, 49 had best matches with the Russulales, and the other had highest similarity with the Thelephoraceae. Our phylogenetic analysis suggests that M. humile roots have a highly specialized association with fungal partners in the Russulaceae. Moreover, a few fungal phylotypes from the M. humile roots had positions neighboring those from Monotropa uniflora roots. These results indicated that the genetic diversity of mycorrhizal fungi of M. humile was highly specific to the Russulaceae, but with high diversity within that family, and that the fungi associated with M. humile differ from those associated with M. uniflora.
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Acknowledgments
We thank Naoto Oonishi (Taiki town office, Mie Prefecture) and Naoya Yamano (The Japan Serow Center in Mie Prefecture) for providing information on the plant materials, the members of the Laboratory of Forest Pathology and Mycology, Mie University, for their assistance in the field, and two anonymous reviewers for their insightful suggestions on this manuscript. This study was supported in part by Grants-in-Aid for Scientific Research from the Japan Ministry of Education, Science, Sports and Culture to YM (20651061 and 22688011).
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Matsuda, Y., Okochi, S., Katayama, T. et al. Mycorrhizal fungi associated with Monotropastrum humile (Ericaceae) in central Japan. Mycorrhiza 21, 569–576 (2011). https://doi.org/10.1007/s00572-011-0365-3
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DOI: https://doi.org/10.1007/s00572-011-0365-3