Abstract
We identified ectomycorrhizal (ECM) roots formed byStrobilomyces confusus associated withAbies firma based on molecular analyses combined with ECM descriptions and examined the correspondence between above-groundS. confusus fruiting bodies and ECM roots below ground. Both mature fruiting bodies ofS. confusus and soil samples (1,000 cm3 in volume) from directly below the fruiting body were collected from three different spots in a 10 × 30-m plot in central Japan, in whichA. firma was the dominant species. Total of 6,156 root tips was collected from the area directly under the fruiting bodies ofS. confusus were classified representing 16 morphotypes. Among them, type 16 was identical in both the length of the internal transcribed spacer region and the restriction fragment length polymorphism profiles ofAlu I orHinf 1 digests, to those of the fruiting body ofS. confusus. Although a specific ECM association betweenS. confusus andA. firma was confirmed by using molecular tools, the abundance of the ECM roots was consistently low in every soil sample tested. These results suggest that even directly beneathS. confusus fruiting bodies, the occurrence of this species above-ground does not accurately reflect its relative abundance below ground, as evaluated in terms of the number of ECM roots.
Similar content being viewed by others
Literature cited
Agerer, R. (1986) Studies on ectomycorrhizae II. Introducing remarks on characterization and identification. Mycotaxon 26: 473–492.
Agerer, R. (1988) Studies on ectomycorrhizae. XIV. Mycorrhizae formed byCortinarius hercynicus andC. variecolor onPicea abies. Can. J. Bot. 66: 2068–2078.
Agerer, R. (1987–1995) Colour atlas of ectomycorrhizae. Einhorn-Verlag Eduard Dietenberger, Schwabisch Gmund, Germany.
Agerer, R. (1995) Anatomical characteristics of identified ectomycorrhizas: An attempt towards a natural classification.In Mycorrhiza: structure, function, molecular biology and biotechnology. Varma, A. and Hock, B. (eds.), 747 pp, Springer-Verlag, Berlin, 685–734.
Bruns, T.D., White, T.J., and Taylor, J.W. (1991) Fungal molecular systematics. Annu. Rev. Ecol. Syst. 22: 525–564.
Chilvers, G.A. (1968) Some distinctive types of eucalypt mycorrhiza. Aust. J. Bot. 16: 49–70.
Dahlberg, A., Jonsson, L., and Nylund, J-E. (1997) Species diversity and distribution of biomass above and below ground among ectomycorrhizal fungi in an old-growth Norway spruce forest in south Sweden. Can J. Bot. 75: 1323–1335.
Danielson, R. M. (1984) Ectomycorrhizal associations in jack pine stands in northeastern Alberta. Can. J. Bot. 62: 932–939.
Dominik, T. (1969) Key to ectotrophic mycorrhizae. Folia For. Polonica Ser. A 15: 309–328.
Egger, K.N. (1995) Molecular analysis of ectomycorrhizal fungal communities. Can. J. Bot. 73 (Suppl. 1): S1415-S1422.
Gardes, M. and Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rust. Mol. Ecol. 2: 113–118.
Gardes, M. and Bruns, T.D. (1996) Community structure of ectomycorrhizal fungi in aPinus muricata forest: above- and below-ground views. Can. J. Bot. 74: 1572–1583.
Goodman, D.M. and Trofymow, J.A. (1998) Comparison of communities of ectomycorrhizal fungi in old-growth and mature stands of Douglas-fir at two sites on southern Vancouver Island. Can. J. For. Res. 28: 574–581.
Harvey, A.E., Larsen, M.J., and Jurgensen, M.F. (1976) Distribution of ectomycorrhizae in a mature Douglas-fir/Larch forest soil in western Montana. Forest Sci. 22: 393–398.
Imazeki, R. and Hongo, T. (1987) Colored illustrations of mushrooms of Japan. Vol. I. 325 pp, Hoikusha, Osaka. (in Japanese)
Ingleby, K., Mason, P.A., Last, F.T., and Fleming, L.V. (1990) Identification of ectomycorrhizas. 112 pp, ITE Res. Publ. No.5, HMSO, London, U.K.
Iwabuchi, S., Sakai, S., and Yamaguchi, O. (1994) Analysis of mushroom diversity in successional young forests and equilibrium evergreen broad-leaved forests. Mycoscience 35: 1–14.
Masui, K. (1926) A study of the mycorrhiza ofAbies firma, S. et Z., with special reference to its mycorrhizal fungus,Cantharellus floccosus, Schw. Mem. Coll. Sci. Kyoto Imp. Univ. Ser. B 2: 15–84.
Matsuda, Y. (1994) Seasonal occurrence and spatial distribution of fruitbodies of ectomycorrhizal fungi on the border of a man-made and a naturally regenerated forest. Bull. Nagoya Univ. For. 13: 109–118.
Matsuda, Y. and Hijii, N. (1998) Spatiotemporal distribution of fruitbodies of ectomycorrhizal fungi in anAbies firma forest. Mycorrhiza 8: 131–138.
Matsuda, Y. and Hijii, H. (1999) Ectomycorrhizal morphotypes of naturally grownAbies firma Sieb. et Zucc. seedlings. Mycoscience 40: in press.
Miller, S.L., Koo, C.D., and Molina, R. (1991) Characterization of red alder ectomycorrhizae: a preface to monitoring belowground ecological responses. Can. J. Bot. 69: 516–531.
Molina, R., Massicotte, H. B., and Trappe, J. M. (1992) Specificity phenomena in mycorrhizal symbioses: Community-ecological consequences and practical implications.In Mycorrhizal Functioning. Allen, M.F. (ed.), 534pp, Chapman & Hall, London, 357–423.
Nagasawa, E. (1997) A preliminary checklist of the Japanese Agaricales. I. The Boletineae. Rep. Tottori Mycol. Inst. 35: 39–78.
Pérez-Silva, E. and León de la Luz, J.L. (1997) Macromycetes of Baja California Sur, Mexico. Mycotaxon 63: 395–417.
Trappe, J.M. (1962) Fungus associates of ectotrophic mycorrhizae. Bot. Rev. 28: 538–606.
Visser, S. (1995) Ectomycorrhizal fungal succession in jack pine stands following wildfire. New Phytol. 129: 389–401.
Yamada, A. and Katsuya, K. (1996) Morphological classification of ectomycorrhizas ofPinus densiflora. Mycoscience 37: 145–155.
Author information
Authors and Affiliations
Additional information
This work was supported in part by a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sport, and Culture (No. 08003234).
About this article
Cite this article
Matsuda, Y., Hijii, N. Characterization and identification ofStrobilomyces confusus ectomycorrhizas on momi fir by RFLP analysis of the PCR-amplified ITS region of the rDNA. J For Res 4, 145–150 (1999). https://doi.org/10.1007/BF02762239
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02762239