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In vitro ectomycorrhizal specificity between the Asian red pine Pinus densiflora and Tricholoma matsutake and allied species from worldwide Pinaceae and Fagaceae forests

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Abstract

Tricholoma matsutake produces commercially valuable, yet uncultivable, mushrooms (matsutake) in association with pines in the Far East and Scandinavia and with both pines and oaks in the foothills of Tibet. Other matsutake mushrooms, such as Tricholoma anatolicum from the Mediterranean regions and Tricholoma magnivelare and Tricholoma sp. from the North Pacific Coast area of Canada and North America as well as Mexico, respectively, are associated with pines or oaks in their natural habitats. Tricholoma bakamatsutake and Tricholoma fulvocastaneum from Asia produce moderately valuable matsutake mushrooms and are solely associated with Fagaceae in nature. In this study, we demonstrate for the first time that matsutake mushrooms from Scandinavia, Mediterranean regions, North America, and Tibet form ectomycorrhizae with Pinus densiflora similar to the Far East T. matsutake. In general, worldwide T. matsutake and the symbionts of Pinaceae colonize the rhizospheres of P. densiflora as well as T. matsutake isolated from the host plant. However, T. fulvocastaneum and T. bakamatsutake formed a discontinuous Hartig net and no Hartig net, respectively, and colonized to a lesser extent as compared to T. matsutake. The data suggest that conifer-associated matsutake mushrooms in their native habitat will associate symbiotically with the Asian red pine.

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Acknowledgments

We thank Hisashi Hada, Fukuzawa Fuminori, and Wanwisa Fangfuk of Shinshu University for technical assistance with the mycorrhization experiment and the DNA sequence of the cultured strains and M. Halil Solak of Muğla University for the guide to field sampling in Turkey. This study was partly supported by a grant-in-aid (no. 19380085) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Minami-minowa, Nagano, 399-4598, Japan

    Akiyoshi Yamada

  2. Ibaraki Prefectural Forestry Institute, Naka, Ibaraki, 311-0122, Japan

    Hisayasu Kobayashi

  3. Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687, Japan

    Hitoshi Murata

  4. Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, İzmir, Turkey

    Erbil Kalmiş

  5. Department of Biology, Faculty of Science and Arts, Celal Bayar University, Muradiye, Manisa, Turkey

    Fatih Kalyoncu

  6. Faculty of Agriculture, Shinshu University, Minami-minowa, Nagano, 399-4598, Japan

    Masaki Fukuda

Authors
  1. Akiyoshi Yamada
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  2. Hisayasu Kobayashi
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  3. Hitoshi Murata
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  4. Erbil Kalmiş
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  5. Fatih Kalyoncu
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  6. Masaki Fukuda
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Correspondence to Akiyoshi Yamada.

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Yamada, A., Kobayashi, H., Murata, H. et al. In vitro ectomycorrhizal specificity between the Asian red pine Pinus densiflora and Tricholoma matsutake and allied species from worldwide Pinaceae and Fagaceae forests. Mycorrhiza 20, 333–339 (2010). https://doi.org/10.1007/s00572-009-0286-6

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