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A critical review of enzymes immobilized on chitosan composites: characterization and applications

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Abstract

Enzymes with industrial significance are typically used in biological processes. However, instability, high sensitivity, and impractical recovery are the major drawbacks of enzymes in practical applications. In recent years, the immobilization technology has attracted wide attention to overcoming these restrictions and improving the efficiency of enzyme applications. Chitosan (CS) is a unique functional substance with biocompatibility, biodegradability, non-toxicity, and antibacterial properties. Chitosan composites are anticipated to be widely used in the near future for a variety of purposes, including as supports for enzyme immobilization, because of their advantages. Therefor this review explores the effects of the chitosan’s structure, molecular weight, degree of deacetylation on the enzyme immobilized, effect of key factors, and the enzymes immobilized on chitosan based composites for numerous applications, including the fields of biosensor, biomedical science, food industry, environmental protection, and industrial production. Moreover, this study carefully investigates the advantages and disadvantages of using these composites as well as their potential in the future.

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Acknowledgements

This research was supported by fund of Natural Fund project of Gansu Province (21JR11RA068) and Industrial Support Plan Project of Gansu Province colleges and universities, No. 2020C-38.

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  1. School of Environment and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, People’s Republic of China

    Yuan Bai, Zongxian Jing, Rui Ma, Xinwen Wan, Jie Liu & Weiting Huang

  2. Key Laboratory of Yellow River Water Environment in Gansu Province, Lanzhou Jiaotong University, Lanzhou, 730070, People’s Republic of China

    Yuan Bai

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Material preparation, data collection and analysis were performed by YB, ZXJ, RM, XWW, JL, and WTH. The first draft of the manuscript was written by ZXJ and conceptualization, supervision, writing—review and editing by YB.

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Bai, Y., Jing, Z., Ma, R. et al. A critical review of enzymes immobilized on chitosan composites: characterization and applications. Bioprocess Biosyst Eng 46, 1539–1567 (2023). https://doi.org/10.1007/s00449-023-02914-0

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