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Evaluation of Monoraphidium contortum for the tertiary treatment of dairy industry wastewater and biomass production with nitrogen supplementation

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Abstract

The present study sought to evaluate the secondary wastewater from dairy industry as a culture medium for Monoraphidium contortum, in bench-scale tubular photobioreactor, aiming at tertiary wastewater treatment and microalgae biomass production. Since the used secondary wastewater contained residual phosphorus (P) but negligible residual nitrogen (N), we also evaluated the nitrogen supplementation, following Redfield ratio (N:P = 16:1) and the same N:P proportion of Bold Medium (N:P = 1.71:1). These cultures were compared to secondary wastewater without N and Bold Medium (control). Secondary wastewater without N addition provided lower values of maximum biomass concentration, indicating the importance of this supplementation. The nitrogen supplementation following Bold Medium represented the best protocol, since biomass productivity was higher than that in control culture, but with lower nitrogen addition (in comparison with the supplementation following Redfield proportion). The biomass of M. contortum showed to be an excellent candidate for oil production, which could be employed as feedstock for biodiesel, for example.

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Fig. 1

Adapted from Rodrigues-Sousa et al. [4] (with kind permission from Elsevier) and Pérez-Mora et al. [20] (with kind permission from John Wiley and Sons)

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Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.

References

  1. Embrapa (2020) Indicadores: Leite e Derivados. Empres Bras Pesqui Agropecuária. 1–16

  2. Machado RMG, Silva PC, Freire VH (2001) Controle ambiental em indústrias de laticínios. Bras Aliment. 7(1):34–36

    Google Scholar 

  3. de Nunes O, Inoue CHB, Rodrigues-Sousa AE et al (2021) Tertiary treatment of dairy industry wastewater with production of Chlorella vulgaris biomass : evaluation of effluent dilution. Brazil J Environ Sci. 56:365–373

    Google Scholar 

  4. Rodrigues-Sousa AE, Nunes IVO, Muniz-Junior AB et al (2021) Nitrogen supplementation for the production of Chlorella vulgaris biomass in secondary effluent from dairy industry. Biochem Eng J. https://doi.org/10.1016/j.bej.2020.107818

    Article  Google Scholar 

  5. Tang EPY, Vincent WF, Proulx D et al (1997) Polar cyanobacteria versus green algae for tertiary waste-water treatment in cool climates. J Appl Phycol 9:371–381. https://doi.org/10.1023/A:1007987127526

    Article  CAS  Google Scholar 

  6. Bogen C, Klassen V, Wichmann J et al (2013) Identification of Monoraphidium contortum as a promising species for liquid biofuel production. Bioresour Technol 133:622–626. https://doi.org/10.1016/j.biortech.2013.01.164

    Article  CAS  Google Scholar 

  7. Pineda-Camacho G, de Guillén-Jiménez FM, Pérez-Sánchez A et al (2019) Effect of CO2 on the generation of biomass and lipids by Monoraphidium contortum: a promising microalga for the production of biodiesel. Bioresour Technol Reports. https://doi.org/10.1016/j.biteb.2019.100313

    Article  Google Scholar 

  8. Kirchner NJ, Hage A, Gomez J et al (2022) Photosynthesis, competition, and wastewater treatment characteristics of the microalga Monoraphidium sp. Dek19 at cool temperatures. Algal Res. https://doi.org/10.1016/j.algal.2021.102624

    Article  Google Scholar 

  9. Kuravi SD, Mohan SV (2022) Mixotrophic cultivation of Monoraphidium sp. In dairy wastewater using Flat-Panel photobioreactor and photosynthetic performance. Bioresour Technol 348:126671. https://doi.org/10.1016/j.biortech.2021.126671

    Article  CAS  Google Scholar 

  10. Hawrot-Paw M, Koniuszy A, Gałczyńska M (2020) Sustainable production of monoraphidium microalgae biomass as a source of bioenergy. Energies. https://doi.org/10.3390/en13225975

    Article  Google Scholar 

  11. Matsudo MC, Sant´ Anna CL, Pérez-Mora LS et al (2021) Evaluation of green microalgae isolated from central and north coast of Sao Paulo as source of oil. J Biotech Biodivers. 9:012–019

    Article  Google Scholar 

  12. UTEX Bold 1NV Medium. https://utex.org/collections/algal-culture-media. Accessed 4 Jan 2021

  13. Becker W (2004) Microalgae in human and animal nutrition. In: Richmond A (ed) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell Science, London, pp 312–351

    Google Scholar 

  14. Koroleff F (1976) Determination of nutrients. In: Grasshoff K (ed) Methods of seawater analysis. Verlag Chemie, Weinheim, pp 117–181

    Google Scholar 

  15. JP Carmouze (1994) O metabolismo dos ecossistemas aquáticos : fundamentos teóricos, métodos de estudo e análises químicas. Edgard Blucher

  16. APHA (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, Washington

    Google Scholar 

  17. Mackereth FJ, Heron J, Talling JF (1978) Water analysis: some revised methods for limnologists. Freshwater Biologic Assoc Ambleside. 36:117

    Google Scholar 

  18. Strickland JD, Parsons T (1960) A manual of seawater analysis. Bull Fish Res Board Canada 125:1–185

    Google Scholar 

  19. Pelizer LH, Sassano CE, Carvalho JCM et al (1999) Padronização do método de secagem da biomassa de Spirulina platensis. Farmácia e Química 32:37–40

    Google Scholar 

  20. Pérez-Mora LS, Matsudo MC, Cezare-Gomes EA, Carvalho JCM (2016) An investigation into producing Botryococcus braunii in a tubular photobioreactor. J Chem Technol Biotechnol 91:3053–3060. https://doi.org/10.1002/jctb.4934

    Article  CAS  Google Scholar 

  21. AOAC (1984) Official methods of analysis of the association of official analytical chemists, 14th edn. American Public Health Association, Arlington

    Google Scholar 

  22. Leduy A, Zajic JE (1973) A geometrical approach for differentiation of an experimental function at a point applied to growth and product formation. Biotechnol Bioeng 25:805–810

    Article  Google Scholar 

  23. Matsudo MC, Sousa TFTF, Pérez-Mora LSLS et al (2017) Ethanol as complementary carbon source in Scenedesmus obliquus cultivation. J Chem Technol Biotechnol 92:781–786. https://doi.org/10.1002/jctb.5059

    Article  CAS  Google Scholar 

  24. Markou G, Vandamme D, Muylaert K (2014) Microalgal and cyanobacterial cultivation: The supply of nutrients. Water Res 65:186–202. https://doi.org/10.1016/j.watres.2014.07.025

    Article  CAS  Google Scholar 

  25. Becker EW (1994) Microalgae Biotechnology and Microbiology. Cambridge University Press, New york

    Google Scholar 

  26. Matsudo MC, Bezerra RP, Sato S et al (2012) Photosynthetic efficiency and CO2 assimilation rate of Arthrospira (Spirulina) platensis continuously cultivated in tubular photobioreactor. Biotechnol J 7:1412–1417

    Article  CAS  Google Scholar 

  27. Matsudo MC, Sant´ Anna CL, Pérez-mora LS et al (2020) Isolation and evaluation of microalgae from mangrove area in south coast of sao paulo (Brazil ) for lipid production. Int J Curr Microbiol Appl Sci. 9:1293–1302

    Article  CAS  Google Scholar 

  28. Tornabene TG, Holzer G, Lien S, Burris N (1983) Lipid composition of the nitrogen starved green alga Neochloris oleoabundans. Enzyme Microb Technol 5:435–440

    Article  CAS  Google Scholar 

  29. Santos AM, Janssen M, Lamers PP et al (2012) Growth of oil accumulating microalga Neochloris oleoabundans under alkaline-saline conditions. Bioresour Technol 104:593–599. https://doi.org/10.1016/j.biortech.2011.10.084

    Article  CAS  Google Scholar 

  30. Knothe G (2006) Analyzing biodiesel : standards and other methods. J Am Oil Chem Soc 83:823–833

    Article  CAS  Google Scholar 

  31. Converti A, Casazza AA, Ortiz EY et al (2009) Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Process Process Intensif 48:1146–1151. https://doi.org/10.1016/j.cep.2009.03.006

    Article  CAS  Google Scholar 

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Funding

This work was supported in part by CNPq (National Council for Scientific and Technological Development, grant 402658/2013–2) and Fapemig (Fundação de Amparo à Pesquisa de Minas Gerais).

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Authors and Affiliations

  1. Institute of Natural Resources, Federal University of Itajubá, Av. Benedito Pereira Dos Santos, Itajubá–M.G, S.P, 1303, Brazil

    Nicole Choi, Ivan V. O. Nunes, Guilherme O. M. Ohira & Marcelo C. Matsudo

  2. Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, Sao Paulo, S.P, 580, Brazil

    João Carlos M. Carvalho

  3. Center for Natural and Human Sciences, Federal University of ABC, Av. Dos Estados, Santo André, S.P, 5001, Brazil

    Marcelo C. Matsudo

Authors
  1. Nicole Choi
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  2. Ivan V. O. Nunes
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  3. Guilherme O. M. Ohira
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  4. João Carlos M. Carvalho
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  5. Marcelo C. Matsudo
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Contributions

MCM and JCMC: contributed to the study conception and design, and funding acquisition. Material preparation, data collection and analysis were performed by NC, IVON and GOMO. The first draft of the manuscript was written by NC and MCM. And all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Marcelo C. Matsudo.

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Choi, N., Nunes, I.V.O., Ohira, G.O.M. et al. Evaluation of Monoraphidium contortum for the tertiary treatment of dairy industry wastewater and biomass production with nitrogen supplementation. Bioprocess Biosyst Eng 46, 265–271 (2023). https://doi.org/10.1007/s00449-022-02831-8

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