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High-sodium maltobionate production by immobilized Zymomonas mobilis cells in polyurethane

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Abstract

The purpose of this study was the production of maltobionic acid, in the form of sodium maltobionate, by Z. mobilis cells immobilized in polyurethane. The in situ immobilized system (0.125–0.35 mm) was composed of 7 g polyol, 3.5 g isocyanate, 0.02 g silicone, and 7 g Z. mobilis cell, at the concentration of 210 g/L. The bioconversion of maltose to sodium maltobionate was performed with different cell concentrations (7.0–9.0 gimobilized/Lreaction_medium), temperature (30.54–47.46 °C), pH (5.55–7.25), and substrate concentration (0.7–1.3 mol/L). The stability of the immobilized system was evaluated for 24 h bioconversion cycles and storage of 6 months. The maximum concentration of sodium maltobionate was 648.61 mmol/L in 34.34 h process (8.5 gdry_cell/Lreaction_medium) at 39 °C and pH 6.30. The immobilized system showed stability for 19 successive operational cycles of 24 h bioconversion and 6 months of storage, at 4 °C or 22 °C.

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Abbreviations

GFOR:

Glucose-fructose oxidoreductase

GL:

Glucono-δ-lactonase

NADP+ :

Nicotinamide adenine dinucleotide phosphate

NADPH:

Dihydro nicotinamide adenine dinucleotide phosphate

PU:

Polyurethane

P:

Mass productivity

q:

Specific productivity

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Acknowledgements

This study was financed in part by the coordination of Improvement of Higher Education Personnel – Brazil (CAPES) – Financing Code 001, National Council for Scientific and Technological Development (CNPq), Research Support Foundation of the State of Rio Grande do Sul (FAPERGS), URI Erechim, and UCS Caxias do Sul.

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Authors and Affiliations

  1. Department of Food Engineering, Universidade Regional Integrada Do Alto Uruguai E das Missões, CEP: 99709-910, Erechim, RS, Brasil

    Roberta Cristina de Souza, Leonardo Meirelles da Silva, Bruna Maria Puton, Eunice Valduga & Jamile Zeni

  2. Biotechnology Institute, Universidade de Caxias Do Sul, CEP: 95070-560, Caxias Do Sul, RS, Brasil

    Sabrina Carra, Maicon Flores & Eloane Malvessi

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  1. Roberta Cristina de Souza
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  2. Leonardo Meirelles da Silva
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  3. Sabrina Carra
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  4. Maicon Flores
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  5. Bruna Maria Puton
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  6. Eloane Malvessi
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Correspondence to Jamile Zeni.

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de Souza, R.C., da Silva, L.M., Carra, S. et al. High-sodium maltobionate production by immobilized Zymomonas mobilis cells in polyurethane. Bioprocess Biosyst Eng 45, 1465–1476 (2022). https://doi.org/10.1007/s00449-022-02756-2

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