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High lactobionic acid production by immobilized Zymomonas mobilis cells: a great step for large-scale process

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A Correction to this article was published on 30 April 2020

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Abstract

Lactobionic acid and sorbitol are produced from lactose and fructose in reactions catalyzed by glucose–fructose oxidoreductase and glucono-δ-lactonase, periplasmic enzymes present in Zymomonas mobilis cells. Considering the previously established laboratory-scale process parameters, the bioproduction of lactobionic acid was explored to enable the transfer of this technology to the productive sector. Aspects such as pH, temperature, reuse and storage conditions of Ca-alginate immobilized Z. mobilis cells, and large-scale bioconversion were assessed. Greatest catalyst performance was observed between pH range of 6.4 and 6.8 and from 39 to 43 °C. The immobilized biocatalyst was reused for twenty three 24-h batches preserving the enzymatic activity. The activity was maintained during biocatalyst storage for up to 120 days. Statistically similar results, approximately 510 mmol/L of lactobionic acid, were attained in bioconversion of 0.2 and 3.0 L, indicating the potential of this technique of lactobionic acid production to be scaled up to the industrial level.

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  • 30 April 2020

    In the original publication, table captions were incorrectly published. The correct captions are given here.

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Acknowledgements

This study was supported by grants from the Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Brazil), Universidade Federal do Rio Grande do Sul (UFRGS), and the Universidade de Caxias do Sul (UCS).

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Authors and Affiliations

  1. Departamento de Farmácia, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, Brazil

    Sabrina Carra, Maria Gabriele Delagustin & Valquiria Linck Bassani

  2. Instituto de Biotecnologia, Universidade de Caxias Do Sul, PO Box 1352, Caxias do Sul, RS, 95001-970, Brazil

    Sabrina Carra, Daniela Cauzzi Rodrigues, Natalia Moreno Conceição Beraldo, Analia Borges Folle, Bruna Campos de Souza, Caroline Reginatto, Tomás Augusto Polidoro, Mauricio Moura da Silveira & Eloane Malvessi

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  1. Sabrina Carra
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Carra, S., Rodrigues, D.C., Beraldo, N.M.C. et al. High lactobionic acid production by immobilized Zymomonas mobilis cells: a great step for large-scale process. Bioprocess Biosyst Eng 43, 1265–1276 (2020). https://doi.org/10.1007/s00449-020-02323-7

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