Abstract
Cultivation of cell suspension culture of Bacopa monnieri targeting the production of bacosides was explored in a 5-l stirred tank reactor using statistically optimized conditions. The bioreactor cultivation conditions were modified and this led to profuse biomass growth (2.81 ± 0.20 g/l) and total bacosides (1.26 ± 0.23 mg/g in cells and 0.60 ± 0.11 mg/l in fermenter broth) production in 9 days. The values of static volumetric mass transfer coefficient (kLa), dimensionless mixing time (Nm) were measured in the bioreactor. The culture grew efficiently and produced enhanced amount of bacoside A (5.59 ± 0.41 mg/g total bacosides in cells and 3.12 ± 0.13 mg/l in the fermenter broth) using one cycle of repeated batch strategy adopted in the bioreactor for 15 days. The intracellular concentration of bacoside A3 (1.18 ± 0.11 mg/g), bacopaside II (2.09 ± 0.35 mg/g), bacopaside X (0.79 ± 0.17 mg/g) and bacopasaponin C (2.24 ± 0.23 mg/g) were significantly higher in repeated batch as compared to batch bioreactor cultivation. The yield of total bacosides in the fermenter broth was 5-times higher in repeated batch as compared to batch cultivation. This strategy can be helpful for the enhanced production of other valuable triterpenoid saponins.
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Acknowledgements
The authors would like to thank CSIR-CIMAP for providing plants of high yielding cultivar of Bacopa monnieri. The authors would like to thank Ministry of Education (Government of India) for providing the masters fellowship for this work to one of the authors (KKS).
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Krishna K. Sahoo and Nivedita Patra performed all the experimental work for this study. Binod B. Sahu and Ved R. Singh were the collaborators for this work and provided resources for this work. Nivedita Patra was the supervisor of Krishna K. Sahoo and also provided guidance for this work.
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Sahoo, K.K., Sahu, B.B., Singh, V.R. et al. Enhanced production of Bacopa saponins by repeated batch strategy in bioreactor. Bioprocess Biosyst Eng 45, 829–841 (2022). https://doi.org/10.1007/s00449-022-02700-4
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DOI: https://doi.org/10.1007/s00449-022-02700-4