Abstract
A cost-effective and efficient protocol has been described in the present work for large scale and rapid in vitro propagation of a valuable medicinal herb Bacopa monnieri (L.) by shoot bud proliferation on internodal segments isolated from field grown mature plants. This shooting was achieved on low concentration of cytokinin with BAP 1.0 mg/l and Kn 0.5 mg/l within 3 weeks of inoculation contrary to earlier reports where auxin is also used in combination with cytokinin. Normally internodes gives rise to a clump of shoots (18 shoots). On an average within a period of three subcultures the internode explant generated 324 shoots thereby improving the economics of the cost of the plants produced and time required. For rooting this is a first report of its growth in a liquid culture. The regenerated plants resemble the mother plants in the general habitat without any morphological variation. This paper reports a very simple, reproducible procedure for in vitro propagation of Bacopa monnieri (L.) that can be used to form a foundation stock of elite plant material for large-scale cultivation.
Similar content being viewed by others
Abbreviations
- BAP:
-
Benzylaminopurine
- MS:
-
Murashige and Skoog medium
- Kn:
-
Kinetin
- NAA:
-
Naphthalene acetic acid
References
Anon (2004) Bacopa monnieri. Monograph. Altern Med Rev 9:79–85
Bafna PA, Balaraman R (2005) Antioxidant activity of DHC-1, an herbal formulation, in experimentally-induced cardiac and renal damage. Phytother Res 19(3):216–221
Banerjee M, Shrivastava S (2006) In vitro regeneration of Jatropha curcas (Ratanjyot): prospects for biofuel production and medicines. Ind J Bot Res 2:195–200
Biondi S, Thorpe TA (1981) Requirement for a tissue culture facility. In: Thorpe TA (ed) Plant tissue culture: methods and applications in agriculture. Academic Press, New York, pp 1–20
Chakravarty AK, Garai S, Masuda K et al (2003) Bacopasides III-V: three new triterpenoid glycosides from Bacopa monniera. Chem Pharm Bull (Tokyo) 51:215–217
Constable F (1990) Medicinal plant biotechnology. Planta Med 56:421–425
Dhyani PP, Kala CP (2005) Current research on medicinal plants: five lesser known but valuable aspects. Curr Sci 88:335
Ernst E (2006) Herbal remedies for anxiety – a systematic review of controlled clinical trials. Phytomedicine 13:205–208
Gamborg OL, Phillips GC (2004) Plant cell tissue and organ culture. Fundamental methods. Narosa Publishing House, New Delhi
Jain P, Khanna NK, Trehan N, Pendse VK, Godhwani JL (1994) Anti-inflammatory effects of an ayurvedic preparation, Brahmi Rasayana in rodents. Ind J Exp Biol 32:633–636
Kala CP (2005) Indigenous uses, population density, and conservation of threatened medicinal plants in protected areas of the Indian Himalayas. Conserv Biol 19:368–378
Kala CP, Dhyani PP, Sajwan BS (2006) Developing the medicinal plants sector in northern India: challenges and opportunities. J Ethnobiol Ethnomed 2:32
Mahato SB, Garai S, Chakravarty AK (2000) Bacosaponins E and F: two jujubogenin bisdesmosides from Bacopa monnieri. Phytochemistry 53:711–714
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
National Medicinal Plant Board (NMPB) (2007) Government of India and Technology Information Forecasting and Assessment Council http://www.nmpb.nic.in/prioritisedmedicinalplants.htm, viewed 12-11-2007
Rastogi S, Mehrotra BN, Kulshreshtha DK (1994) In: Deep publications (ed) Proceedings of IV international congress of ethnobiology, New Delhi, p 93
Russo A, Borrelli F (2005) Bacopa monniera, a reputed nootropic plant: an overview. Phytomedicine 12(4):305–317
Satyavati GV, Raina MK, Sharma M (1976) Indian medicinal plants, vol 1. Indian Council of Medical Research, New Delhi, pp 20–35
Sekar P (1996) Vedic clues to memory enhancer. The Hindu, March 21
Shrivastava N, Rajni M (1999) Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell. Plant cell Rep 18:919–923
Sinha S, Saxena R (2006) Effect of iron on lipid peroxidation and enzymatic and non-enzymatic antioxidants and bacoside—a content in medicinal plant Bacopa monniera L. Chemosphere 62:1340–1350
Sivaramakrishna C, Rao CV, Trimurtulu G et al (2005) Triterpenoid glycosides from Bacopa monniera. Phytochemistry 66:2719–2728
Tripathi YB, Chaurasia S, Tripathi E, Upadhaya A, Dubey GP (1996) Bacopa monnieri Linn as an antioxidant: mechanism of action. Ind J Exp Biol 34:521–526
Vieira RF, Skorupa LA (1993) Brazilian medicinal plants gene bank. Acta Hort 330:51–58
Wakhlu AK, Bajwa PS (1986) Regeneration of uniform plants from somatic embryos of Papaver somniferum (opium poppy). Phytomorphology 36:101–105
Acknowledgments
The authors are grateful to the Department of Bioscience for the necessary laboratory facilities.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Banerjee, M., Shrivastava, S. An improved protocol for in vitro multiplication of Bacopa monnieri (L.). World J Microbiol Biotechnol 24, 1355–1359 (2008). https://doi.org/10.1007/s11274-007-9612-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-007-9612-3