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Comparative performance and reusability studies of lipases on syntheses of octyl esters with an economic approach

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Abstract

A suitable immobilized lipase for esters syntheses should be selected considering not only its cost. We evaluated five biocatalysts in syntheses of octyl caprylate, octyl caprate, and octyl laurate, in which conversions higher than 90% were achieved. Novozym® 435 and non-commercial preparations (including a dry fermented solid) were selected for short-term octyl laurate syntheses using different biocatalysts loadings. By increasing the biocatalyst’s loading the lipase’s reusability also raised, but without strict proportionality, which resulted in a convergence between the lowest biocatalyst loading and the lowest cost per batch. The use of a dry fermented solid was cost-effective, even using loadings as high as 20.0% wt/wt due to its low obtaining cost, although exhibiting low productiveness. The combination of biocatalyst’s cost, esterification activity, stability, and reusability represents proper criteria for the choice. This kind of assessment may help to establish quantitative goals to improve or to develop new biocatalysts.

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Acknowledgements

The authors would like to thank CNPq (National Council for Scientific and Technological Development—Brazil), FAPERJ (Fundação Carlos Chagas Filho de Apoio à Pesquisa do Estado do Rio de Janeiro) and MCTI (Ministry of Science, Technology, and Innovations—Brazil).

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Authors and Affiliations

  1. Biocatalysis Laboratory, Ministry of Science, Technology, and Innovations, National Institute of Technology, Rio de Janeiro, RJ, 20081-312, Brazil

    Ronaldo Rodrigues de Sousa, Ayla Sant’Ana da Silva & Viridiana Santana Ferreira-Leitão

  2. Federal University of Rio de Janeiro, Chemical Engineering Program, COPPE, Rio de Janeiro, RJ, 21941-972, Brazil

    Martina Costa Cerqueira Pinto & José Carlos Pinto

  3. Department of Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil

    Ronaldo Rodrigues de Sousa, Martina Costa Cerqueira Pinto, Erika Cristina Gonçalves Aguieiras, Eliane Pereira Cipolatti, Ayla Sant’Ana da Silva, Denise Maria Guimarães Freire & Viridiana Santana Ferreira-Leitão

  4. Federal University of Rio de Janeiro Campus, UFRJ - Duque de Caxias, Prof. Geraldo Cidade, Duque de Caxias, RJ, 25240-005, Brazil

    Erika Cristina Gonçalves Aguieiras

  5. Pharmaceutical Biotechnology Program, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-972, Brazil

    Eliane Pereira Cipolatti & Evelin Andrade Manoel

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  1. Ronaldo Rodrigues de Sousa
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de Sousa, R.R., Pinto, M.C., Aguieiras, E.C.G. et al. Comparative performance and reusability studies of lipases on syntheses of octyl esters with an economic approach. Bioprocess Biosyst Eng 45, 131–145 (2022). https://doi.org/10.1007/s00449-021-02646-z

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