The effect of growth rate on the production and vitality of non-Saccharomyces wine yeast in aerobic fed-batch culture

Abstract

Non-Saccharomyces wine yeasts are of increasing importance due to their influence on the organoleptic properties of wine and thus the factors influencing the biomass production of these yeasts, as starter cultures, are of commercial value. Therefore, the effects of growth rates on the biomass yield (Y_x/s) and fermentation performance of non-Saccharomyces yeasts at bench and pilot scale were examined. The fermentative performance and (Y_x/s) were optimised, in aerobic fed-batch cultivations, to produce commercial wine seed cultures of Lachancea thermotolerans Y1240, Issatchenkia orientalis Y1161 and Metschnikowia pulcherrima Y1337. Saccharomyces cerevisiae (Lalvin EC1118) was used as a benchmark. A Crabtree positive response was shown by L. thermotolerans in a molasses-based industrial medium, at growth rates exceeding 0.21 h⁻¹ (µ_crit), resulting in a Y_x/s of 0.76 g/g at 0.21 h⁻¹ (46% of µ_max) in the aerobic bioreactor-grown fed-batch culture at bench scale. At pilot scale and 0.133 h⁻¹ (36% of µ_max), this yeast exhibited ethanol concentrations reaching 10.61 g/l, as a possible result of substrate gradients. Crabtree negative responses were observed for I. orientalis and M. pulcherrima resulting in Y_x/s of 0.83 g/g and 0.68 g/g, respectively, below 32% of µ_max. The Y_x/s of M. pulcherrima, I. orientalis and L. thermotolerans was maximised at growth rates between 0.10 and 0.12 h⁻¹ and the fermentative capacity of these yeasts was maximised at these lower growth rates.