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Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors

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Abstract

Lipases CAL-B, TLL, and RML were used in the synthesis of free fatty acids of grape seed oil as heterogeneous substrate. The best enzyme was used to optimize the reaction variables temperature, enzyme content, and molar ratio of water:oil in batch reactions using experimental planning. The ideal conditions to produce free fatty acids using pure RML were 45 °C, 12:1 substrate molar ratio, and 15% enzyme, resulting in 66% of oil hydrolysis and a productivity of 0.54 mol L−1 min−1 in 4 h of reaction at 180 rpm. Repeated batches of reaction were performed testing the operational stability of RML, results showing that this enzyme could be used for at least 20 cycles keeping more than 80% of its initial activity, suggesting its potential use in industrial processes. The synthesis of free fatty acids was then evaluated in continuous reactions using packed-bed reactor (PBR). The highest productivity in the continuous process was 6.85 mol L−1 min−1, using only RML, showing an operational stability higher than 80% of its initial conversion capacity after 11 days of operation, at a flow rate of 0.13 mL min−1 at 45 °C. We evaluated the use of this hydrolyzed oil as substrate for lactone bioproduction using Galactomyces geotrichum UFMG-CM-Y3276, G. geotrichum UFMG-CM-Y3558, and Geotrichum klebahnii UFMG-CM-Y3014 screened for their oil-hydrolysis ability. Volatile compounds were qualitatively identified in GC–MS as γ-octalactone and γ-nonalactone.

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Acknowledgements

This work was supported by grants from Brazilian Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, scholarships for the first and fifth author), Finance Code 001, and grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Grant # 442516/2014-2 for the corresponding author), and from Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS). The authors would like to thank Mr. Ramiro Martinez (Novozymes, Spain S.A.) for kindly supplying the enzymes used in this research.

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  1. Gabriele Zanota Castiglioni, Giulia Bettio and Carla Roberta Matte contributed equally to this manuscript.

Authors and Affiliations

  1. Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil

    Gabriele Zanota Castiglioni, Giulia Bettio, Carla Roberta Matte & Marco Antônio Záchia Ayub

  2. Chemistry Institute, Federal University of Rio Grande Do Sul, Porto Alregre, RS, Brazil

    Rosangela Assis Jacques & Allan Dos Santos Polidoro

  3. Department of Microbiology, ICB, C.P. 486, State University of Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

    Carlos Augusto Rosa

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  1. Gabriele Zanota Castiglioni
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Castiglioni, G.Z., Bettio, G., Matte, C.R. et al. Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors. Bioprocess Biosyst Eng 43, 1391–1402 (2020). https://doi.org/10.1007/s00449-020-02334-4

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