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Optimization of free fatty acid production by enzymatic hydrolysis of vegetable oils using a non-commercial lipase from Geotrichum candidum

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Abstract

This study aimed to optimize free fatty acid production by enzymatic hydrolysis of cottonseed, olive and palm kernel oils in stirred-tank reactors using a lipase from Geotrichum candidum (GCL-I). The effect of pH, temperature and substrate concentration on the hydrolytic activity of GCL-I using these vegetable oils was investigated. Thermal stability tests and thermodynamic studies were also performed. A complete hydrolysis of cottonseed oil was obtained after 120 min of reaction, while for olive and palm kernel maximum hydrolysis percentage was 96.4% and 60.1%, respectively. GCL-I exhibited the highest activity in the hydrolysis of vegetable oils that are rich in unsaturated-fatty acids (cottonseed and olive oils). Under optimal conditions (46.8% m/m of oil, 6.6 U/g of the reaction mixture at 40 °C), complete cottonseed oil hydrolysis was observed at 60 min of reaction performed in an emulsifier-free system with no buffer.

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References

  1. Murty VR, Bhat J, Muniswaran PKA (2002) Hydrolysis of oils using immobilized lipase enzyme: a review. Biotechnol Bioprocess Eng 7:57–66

    Article  CAS  Google Scholar 

  2. Farile JM, Garcia JI, Herrerías CI, Pires E (2017) Synthetic transformations for the valorization of fatty acid derivatives. Synthesis 49:1444–1460

    Article  CAS  Google Scholar 

  3. Santos KC, Cassimiro DMJ, Avelar MHM, Hirata DB, Castro HF, Fernández-Lafuente R, Mendes AA (2013) Characterization of the catalytic properties of lipases from plant seeds for the production of concentrated fatty acids from different vegetable oils. Ind Crops and Prod 49:462–470

    Article  CAS  Google Scholar 

  4. Chen W, Sun S, Liang S, Peng L, Wang Y, Shen M (2014) Lipase-catalysed hydrolysis of linseed oil: optimization using response surface methodology. J Oleo Sci 63:619–628

    Article  CAS  PubMed  Google Scholar 

  5. Satyarthi JK, Srinivas D, Ratnasamy P (2011) Hydrolysis of vegetable oils and fats acids over solid acid catalysts. Appl Catal A Gener 391:427–435

    Article  CAS  Google Scholar 

  6. Holliday RL, King JW, List GR (1997) Hydrolysis of vegetable oils in sub- and supercritical water. Ind Eng Chem Res 36:932–935

    Article  CAS  Google Scholar 

  7. Pinto JSS, Lanças FM (2006) Hydrolysis of corn oil using subcritical water. J Braz Chem Soc 17:85–89

    Article  CAS  Google Scholar 

  8. Fu X, Zhu X, Gao K, Duan J (1995) Oil and fat hydrolysis with lipase from Aspergillus sp. J Am Oil Chem Soc 72:527–531

    Article  CAS  Google Scholar 

  9. Goswami D, Basu JK, De S (2013) Lipase applications in oil hydrolysis with a case study on castor oil: a review. Crit Rev Biotechnol 33:81–96

    Article  CAS  PubMed  Google Scholar 

  10. Almeida AF, Terrasan CRF, Terrone CC, Tauk-Tornisieloc SM, Carmona EC (2018) Biochemical properties of free and immobilized Candida viswanathii lipase on octyl-agarose support: hydrolysis of triacylglycerol and soy lecithin. Process Biochem 65:71–80

    Article  CAS  Google Scholar 

  11. Verger R (1997) Interfacial activation of lipases: facts and artifacts. Trends Biotechnol 15:32–38

    Article  CAS  Google Scholar 

  12. Aziz M, Husson F, Kermasha S (2015) Optimization of the hydrolysis of safflower oil for the production of linoleic acid, used as flavor precursor. Int J Food Sci 2015:1–10

    Article  CAS  Google Scholar 

  13. Yigitoglu M, Temoçin Z (2010) Immobilization of Candida rugosa lipase on glutaraldehyde-activated polyester fiber and its application for hydrolysis of some vegetable oils. J Mol Catal B Enzym 66:130–135

    Article  CAS  Google Scholar 

  14. Serri NA, Kamarudin AH, Rahaman SNA (2008) Preliminary studies for production of fatty acids from hydrolysis of cooking palm oil using C. rugosa lipase. J Phys Sci 19:79–88

    CAS  Google Scholar 

  15. Liu Y, Jin Q, Shan L, Liu Y, Shen W, Wang X (2008) The effect of ultrasound on lipase-catalyzed hydrolysis of soy oil in solvent-free system. Ultrason Sonochem 15:402–407

    Article  CAS  PubMed  Google Scholar 

  16. Avelar MHM, Cassimiro DMJ, Santos KC, Domingues RCC, Castro HF, Mendes AA (2013) Hydrolysis of vegetable oils catalyzed by lipase extract powder from dormant castor bean seeds. Ind Crops Prod 44:452–458

    Article  CAS  Google Scholar 

  17. Stránský K, Zarevúcka M, Kejík Z, Wimmer Z, Macková M, Demnerová K (2007) Substrate specificity, regioselectivity and hydrolytic activity of lipases activated from Geotrichum sp. Biochem Eng J 34:209–216

    Article  CAS  Google Scholar 

  18. Gunstone FD (2002) Vegetable oils in food technology: composition, properties and uses. Blackwell, London

    Google Scholar 

  19. Baillargeon MW, Bistline RG Jr, Sonnet PE (1989) Evaluation of strains of Geotrichum candidum for lipase production and fatty acid specificity. Appl Microbiol Biotechnol 30:92–96

    Article  CAS  Google Scholar 

  20. Alves JS, Vieira NS, Cunha AC, Silva AM, Ayub MAZ, Fernandez-Lafuente R, Rodrigues RC (2014) Combi-lipase for heterogeneous substrates: a new approach for hydrolysis of soybean oil using mixtures of biocatalysts. RSC Adv 4:63–68

    Article  Google Scholar 

  21. Poppe JK, Matte CR, Fernandez-Lafuente R, Rodrigues RC, Ayub MAZ (2018) Transesterification of waste frying oil and soybean oil by combi-lipases under ultrasound-assisted reactions. Appl Biochem Biotechnol 186:576–589

    Article  CAS  PubMed  Google Scholar 

  22. Poppe JK, Matte CR, de Freitas VO, Fernandez-Lafuente R, Rodrigues RC, Ayub MAZ (2018) Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug-flow packed-bed continuous reactor. Biotechn Progress 34:952–959

    Article  CAS  Google Scholar 

  23. Poppe JK, Matte CR, Do Carmo Ruaro Peralba M, Fernandez-Lafuente R, Rodrigues RC, Ayub MAZ (2015) Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases. Appl Catal A Gen 490:50–56

    Article  CAS  Google Scholar 

  24. Sidebottom CM, Charton E, Dunn PPJ, Mycock G, Davies C, Sutton JL, Macrae AR, Slabas AR (1991) Geotrichum candidum produces several lipases with markedly different substrate specificities. Eur J Biochem 202:485–491

    Article  CAS  PubMed  Google Scholar 

  25. Brabcová J, Deminanová Z, Vondrásek J, Jágr M, Zarevúcka M, Palomo JM (2013) Highly selective purification of three lipases from Geotrichum candidum 4013 and their characterization and biotechnological applications. J Mol Catal B Enzym 98:62–72

    Article  CAS  Google Scholar 

  26. Castro PF, Moreira NC, Esperança MN, Oliveira LM, Badino AC, Tavano OL, Mendes AA, Basso RC, Fernández-Lafuente R, Hirata DB (2016) High lipase production from Geotrichum candidum in reduced time using cottonseed oil: optimization, easy purification and specificity characterization. J Chem Eng Res Updates 2016:60–69

    Google Scholar 

  27. Jacobsen T, Olse J, Allermann K (1998) Production, partial purification, and immunochemical characterization of multiple forms of lipase from Geotrichum candidum. Enzyme MicrobTechnol 11:90–95

    Article  Google Scholar 

  28. Diks RMM, Lee ML (1999) Production of a very low saturate oil based on the specificity of Geotrichum candidum lipase. JAOCS 76:455–462

    Article  CAS  Google Scholar 

  29. Foglia TA, Jones KC, Sonnet PE (2000) Selectivity of lipases: isolation of fatty acids from castor, coriander, and meadowfoam oils. Eur J Lipid Sci Technol 102:612–617

    Article  CAS  Google Scholar 

  30. Saleem M, Rashid MH, Jabbar A, Perveen R, Khalid AM, Rajoka MI (2005) Kinetic and thermodynamic properties of an immobilized endoglucanase from Arachniotus citrinus. Process Biochem 40:849–855

    Article  CAS  Google Scholar 

  31. Siddiqui KS, Aala A, Saqib N, Rashid MH (2000) Carboxyl group modification significantly altered the kinetic properties of purified carboxymethylcellulase from Aspergillus niger. Enzyme Microb Technol 27:467–474

    Article  CAS  PubMed  Google Scholar 

  32. Rooney D, Weatherley LR (2001) The effect of reaction conditions upon lipase catalysed hydrolysis of high oleate sunflower oil in a stirred liquid-liquid reactor. Process Biochem 36:947–953

    Article  CAS  Google Scholar 

  33. Sadana A, Henley JP (1987) Single-step unimolecular non-first-order enzyme deactivation kinetics. Biotechnol Bioeng 30:717–723

    Article  CAS  PubMed  Google Scholar 

  34. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  35. Borgstrom B (1952) Investigation on lipid separation methods: separation of cholesterol esters, glycerides and free fatty acids. Acta Physiol Scand 25:111–119

    Article  CAS  PubMed  Google Scholar 

  36. Belfrage P, Vaughan M (1969) Simple liquid–liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. J Lipid Res 10:341–344

    CAS  PubMed  Google Scholar 

  37. Hartman L, Lago RCA (1973) Rapid preparation of fatty acid methyl esters from lipids. Lab Pract 22:475–476

    CAS  PubMed  Google Scholar 

  38. EN 14103 (2011) Fat and oil derivatives—fatty acid methyl esters (FAME)—determination of ester and linolenic acid methyl ester contents

  39. Benzonana G, Desnuelle P (1958) Action de la lipase pancréatique sur les esters en émulsions. Biochim Biophys Acta 30:513–521

    Article  Google Scholar 

  40. Borel P, Armand M, Ythier P, Dutot G, Melin C, Senft M, Lafont H, Lairon D (1994) Hydrolysis of emulsions with different triglycerides and droplet sizes by gastric lipase in vitro. Effect on pancreatic lipase activity. J Nutr Biochem 5:124–133

    Article  CAS  Google Scholar 

  41. Tiss A, Carriéri F, Douchet I, Patkar S, Svendsen AE, Verger R (2002) Interfacial binding and activity of lipases at the lipid–water interface: effects of gum arabic and surface pressure. Colloid Surf B Bioint 26:135–145

    Article  CAS  Google Scholar 

  42. McClements DJ (2005) Food emulsions: principles, practice, and techniques. CRC Press, Boca Raton

    Google Scholar 

  43. Mun S, Decker EA, McClements DJ (2007) Influence of emulsifier type on in vitro digestibility of lipid droplets by pancreatic lipase. Food Res Int 40:770–781

    Article  CAS  Google Scholar 

  44. Bressani APP, Garcia KCA, Hirata DB, Mendes AA (2015) Production of alkyl esters from macaw palm oil by a sequential hydrolysis/esterification process using heterogeneous biocatalysts: optimization by response surface methodology. Bioprocess Biosyst Eng 38:287–297

    Article  CAS  PubMed  Google Scholar 

  45. Saqib AAN, Hassan M, Khan NF, Baig S (2010) Thermostability of crude endoglucanase from Aspergillus fumigatus grown under solid state fermentation (SSF) and submerged fermentation (SmF). Process Biochem 45:641–646

    Article  CAS  Google Scholar 

  46. Souza PM, Aliakbarian B, Ferreira Filho EX, Magalhães PO, Pessoa Junior A, Converti A, Perego P (2015) Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus. Int J Biol Macromol 81:17–21

    Article  CAS  PubMed  Google Scholar 

  47. Marangoni AG (2003) Enzyme Kinetics: a modern approach. Wiley, New Jersey

    Google Scholar 

  48. Miranda JS, Silva NCA, Bassi JJ, Corradini MCC, Lage FAP, Hirata DB, Mendes AA (2014) Immobilization of Thermomyces lanuginosus lipase on mesoporous poly-hydroxybutyrate particles and application in alkyl esters synthesis: isotherm, thermodynamic and mass transfer studies. Chem Eng J 251:392–403

    Article  CAS  Google Scholar 

  49. Cavalcanti-Oliveira EA, Silva PR, Ramos AP, Aranda DAG, Freire DMG (2010) study of soybean oil hydrolysis catalyzed by Thermomyces lanuginosus lipase and its application to biodiesel production via hydroesterification. Enzyme Res 2011:1–8

    Article  CAS  Google Scholar 

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Acknowledgements

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Finance Code 001. The authors are grateful to Fundação de Amparo à Pesquisa do Estado de Minas Gerais-FAPEMIG (TEC-APQ-02755-16) for its financial support. MSc. Matheus M. Ferreira thanks CAPES for his student fellowship. Gustavo F. Oliveira thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for his IC fellowship.

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Authors and Affiliations

  1. Graduate Program in Chemical Engineering, Federal University of Alfenas, Poços de Caldas, MG, 37715-400, Brazil

    Matheus M. Ferreira, Rodrigo C. Basso & Daniela B. Hirata

  2. Institute of Chemistry, Federal University of Alfenas, 700 Gabriel Monteiro da Silva St., Alfenas, MG, 37130-001, Brazil

    Gustavo F. de Oliveira, Adriano A. Mendes & Daniela B. Hirata

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  1. Matheus M. Ferreira
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  2. Gustavo F. de Oliveira
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  3. Rodrigo C. Basso
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Correspondence to Daniela B. Hirata.

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Ferreira, M.M., de Oliveira, G.F., Basso, R.C. et al. Optimization of free fatty acid production by enzymatic hydrolysis of vegetable oils using a non-commercial lipase from Geotrichum candidum. Bioprocess Biosyst Eng 42, 1647–1659 (2019). https://doi.org/10.1007/s00449-019-02161-2

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