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Development of eco-friendly process for the production of bioethanol from banana peel using inhouse developed cocktail of thermo-alkali-stable depolymerizing enzymes

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Abstract

Conversion of agro-industrial wastes to energy is an innovative approach for waste valorization and management which also mitigates environmental pollution. In this view, present study investigated the feasibility of producing bioethanol from banana peels using cocktail of depolymerizing enzyme/s. We isolated Geobacillus stearothermophilus HPA19 from natural resource which produces cocktail of thermo-alkali-stable xylano-pectino-cellulolytic enzyme/s using wheat bran within 24 h. The optimal temperature and pH for xylanase, filter paper cellulase and pectinase were 80, 70 and 80 °C, and 9.0, 8.0 and 9.0, respectively. Cocktail enzymes showed stability at high temperature (80 °C) and pH (10.0). Ni2+ and Zn2+ promoted the relative activity of xylanase and FPase, whereas Na+, Ca2+ and K+ promoted pectinase activity. Cocktail was assessed in saccharification of banana peel. Reducing sugar obtained (37.06 mg ml−1) after one variable at a time (OVAT) method is greatly influenced by enzyme dose. Further, response surface methodology was used to optimize saccharification leading to twofold increase in reducing sugar. Maximum ethanol production (21.1 gl−1) was achieved through fermentation giving the efficiency of 76.5% within 30 h. Hence utilization of waste biomass for production of value-added products through biotechnological intervention not only helps to combat environmental pollution but also contributes significantly to the economy.

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References

  1. Okonkwo CC, Azam MM, Ezeji TC, Qureshi N (2016) Enhancing ethanol production from cellulosic sugars using Scheffersomyces (Pichia) stipitis. Bioproc. Biosyst Eng 39(7):1023–1032

    Article  CAS  Google Scholar 

  2. Dadheech T, Shah R, Pandit R, Hinsu A, Chauhan PS, Jakhesara S, Kunjadiya A, Rank D, Joshi C (2018) Cloning, molecular modeling and characterization of acidic cellulase from buffalo rumen and its applicability in saccharification of lignocellulosic biomass. Int J Biol Macromol 113:73–81

    Article  CAS  PubMed  Google Scholar 

  3. Saxena A, Chauhan PS (2017) Role of various enzymes in deinking of paper: a review. Cri Rev Biotechnol 37(5):598–612

    Article  CAS  Google Scholar 

  4. Nikolić S, Pejin J, Mojović L (2016) Challenges in bioethanol production: utilization of cotton fabrics as a feedstock. Chem Ind Chem Eng Q 22(4):375–390

    Article  Google Scholar 

  5. Hossain N, Zaini JH, Mahlia TMI (2017) A review of bioethanol production from waste based biomass by yeast fermentation. Int J Technol 8(1):5–18

    Article  Google Scholar 

  6. Chauhan PS, Goradia B, Jha B (2018) Optimization and up-scaling of ionic liquid tolerant, thermo-alkali stable extracellular laccase from marine Staphylococcus arlettae S1-20 using tea waste. J Taiwan Inst Chem Engg https://doi.org/10.1016/j.jtice.2018.02.032

    Article  Google Scholar 

  7. Chauhan PS, Puri N, Sharma P, Gupta N (2012) Mannanases: microbial sources, production, properties and potential biotechnological applications. Appl Microbiol Biotechnol 93(5):1817–1830

    Article  CAS  PubMed  Google Scholar 

  8. Chakraborty S, Gupta R, Jain KK, Kuhad RC (2016) Cost-effective production of cellulose hydrolysing enzymes from Trichoderma sp. RCK65 under SSF and its evaluation in saccharification of cellulosic substrates. Bioproc Biosyst Eng 39(11):1659–1670

    Article  CAS  Google Scholar 

  9. Dai Y, Zhang HS, Huan B, He Y (2017) Enhancing the enzymatic saccharification of bamboo shoot shell by sequential biological pretreatment with Galactomyces sp. CCZU11-1 and deep eutectic solvent extraction. Bioproc Biosyst Eng 40(9):1427–1436

    Article  CAS  Google Scholar 

  10. Sharma N, Kalra KL, Oberoi HS, Bansal S (2007) Optimization of fermentation parameters for production of ethanol from kinnow waste and banana peels by simultaneous saccharification and fermentation. Ind J Microbiol 47:310–316

    Article  CAS  Google Scholar 

  11. Oberoi HS, Vadlani PV, Saida L, Bansal S, Hughes JD (2011) Ethanol production from banana peels using statistically optimized simultaneous saccharification and fermentation process. Waste Manag 31:1576–1584

    Article  CAS  PubMed  Google Scholar 

  12. Oberoi HS, Sandhua SK, Vadlani PV (2012) Statistical optimization of hydrolysis process for banana peels using cellulolytic and pectinolytic enzymes. Food Bioproduct Process 90:257–265

    Article  CAS  Google Scholar 

  13. Centre for Monitoring Indian Economy Pvt, Ltd. (2001) Andheri, Mumbai

  14. Shah MP, Reddy GV, Banerjee R, Ravindra P, Kothari IL (2005) Microbial degradation of banana waste under solid state bioprocessing using two lignocellulolytic fungi (Phyllosticta spp. MPS-001 and Aspergillus spp. MPS-002). Process Biochem 40:445–451

    Article  CAS  Google Scholar 

  15. Reddy YAK, Srijana M, Reddy DM, Reddy G (2010) Co-culture fermentation of banana agro-waste to ethanol by cellulolytic thermophilic Clostridium thermocellum CT2. Afr J Biotechnol 9(13):1926–1934

    Article  Google Scholar 

  16. Chauhan PS, Sharma P, Puri N, Gupta N (2014) A process for reduction in viscosity of coffee extract by enzymatic hydrolysis of mannan. Bioproc Biosyst Eng 37(7):1459–1467

    Article  CAS  Google Scholar 

  17. Chauhan PS, Gupta N (2017) Insight into microbial mannosidase: a review. Cri Rev Biotechnol 37(2):190–201

    Article  CAS  Google Scholar 

  18. Gabhane J, William SPMP., Gadhe A, Rath R, Vaidya AN, Wate S (2014) Pretreatment of banana agricultural waste for bio-ethanol production: individual and interactive effects of acid and alkali pretreatments with autoclaving, microwave heating and ultrasonication. Waste Manag 34:498–503

    Article  CAS  PubMed  Google Scholar 

  19. Turner P, Mamo G, Karlsson EN (2007) Potential and utilization of thermophiles and thermostable enzymes in biorefining. Microb Cell Fact 6:9. https://doi.org/10.1186/1475-2859-6-9

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Arumugam R, Manikandan M (2011) Fermentation of pretreated hydrolyzates of banana and mango fruit wastes ethanol production. Asian J Exp Biol Sci 2:246–256

    CAS  Google Scholar 

  21. Ingale S, Joshi SJ, Gupte A (2014) Production of bioethanol using agricultural waste: banana pseudo stem. Braz J Microbiol 45(3):885–892

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Waghmare AG, Arya SS (2016) Utilization of unripe banana peel waste as feedstock for ethanol production. Bioethanol 2:146–156

    Article  CAS  Google Scholar 

  23. Gebregergs A, Gebresemati M, Sahu O (2016) Industrial ethanol from banana peels for developing countries: response surface methodology. Pac Sci Rev A Nat Sci Eng 18(1):22–29

    Google Scholar 

  24. Danmaliki GI, Muhammad AM, Shamsuddeen AA, Usman BJ (2016) Bioethanol production from banana peels. IOSR. J Environ Sci Tox Food Technol 10:56–62

    CAS  Google Scholar 

  25. Ghimire S, Bhattarai S, Phuyal S, Thapa B, Shrestha BG (2016) Isolation and screening of potential cellulolytic and xylanolytic bacteria from soil sample for degradation of lignocellulosic biomass. J Trop Life Sci 6(3):165–169

    Google Scholar 

  26. Chauhan PS, Soni SK, Sharma P, Saini A, Gupta N (2014) A mannanase from Bacillus nealsonii PN-11: statistical optimization of production and application in biobleaching of pulp in combination with xylanase. Int J Pharma Bio Sci 5:237–251

    CAS  Google Scholar 

  27. Reda AB, Hesham MY, Mahmoud AS, Ebtsam ZA (2008) Production of bacterial pectinase(s) from agro-industrial wastes under solid state fermentation conditions. J Appl Sci Res 41:1708–1721

    Google Scholar 

  28. Samira M, Mohammad R, Gholamreza G (2011) Carboxymethyl-cellulase and filter-paperase activity of new strain isolated from persian gulf. Microbiol J 1:8–16

    Article  Google Scholar 

  29. David A, Chauhan PS, Kumar A, Angural S, Kumar D, Puri N, Gupta N (2017) Coproduction of protease and mannanase from Bacillus nealsonii PN-11 in solid state fermentation and their combined application as detergent additives. Int J Biol Macromol https://https://doi.org/10.1016/j.ijbiomac.2017.09.037

    Article  Google Scholar 

  30. Chauhan PS, Tripathi SP, Sangamwar AT, Puri N, Sharma P, Gupta N (2015) Cloning, molecular modeling and docking analysis of alkali-thermostable β-mannanase from Bacillus nealsonii PN-11. Appl Microbiol Biotechnol 99:8917–8925

    Article  CAS  PubMed  Google Scholar 

  31. Chauhan PS, Goradia B, Saxena A (2017) Bacterial laccase: recent update on production and industrial applications. 3Biotech 7:323

    Google Scholar 

  32. Chauhan PS, Saxena A (2016) Bacterial carrageenases: an overview of production and biotechnological applications. 3Biotech 6(2):146

    Google Scholar 

  33. Ahlawat S, Battan B, Dhiman SS, Sharma J, Mandhan RP (2007) Production of thermostable pectinase and xylanase for their potential application in bleaching of kraft pulp. J Ind Microbiol Biotechnol 34(12):763–770

    Article  CAS  PubMed  Google Scholar 

  34. Kaur A, Mahajan R, Singh A, Garg G, Sharma J (2010) Application of cellulase-free xylano-pectinolytic enzymes from the same bacterial isolate in biobleaching of kraft pulp. Bioresour Technol 101:9150–9155

    Article  CAS  PubMed  Google Scholar 

  35. Singh A, Kaur A, Dua A, Mahajan R (2015) An efficient and improved methodology for the screening of industrially valuable xylano–pectino–cellulolytic microbes. Enz Res. https://doi.org/10.1155/2015/725281

  36. Salim AA, Grbavcic S, Šekuljica N, Stefanovic A, Tanaskovic SJ, Lukovic N, Jugovic ZK (2017) Production of enzymes by a newly isolated Bacillus sp. TMF-1 in solid state fermentation on agricultural by-products: the evaluation of substrate pretreatment methods. Bioresour Technol 228:193–200

    Article  CAS  PubMed  Google Scholar 

  37. Seo JK, Park TS, Kwon IH, Piao MY, Lee CH, Jong KH (2013) Characterization of cellulolytic and xylanolytic enzymes of Bacillus licheniformis JK7 isolated from the rumen of a native korean goat. Asian Aust J Anim Sci 26:50–58

    Article  CAS  Google Scholar 

  38. Song JM, Wei DZ (2010) Production and characterization of cellulases and xylanases of Cellulosimicrobium cellulans grown in pretreated and extracted bagasse and minimal nutrient medium M9. Biomass Bioeng 34(12):1930–1934

    Article  CAS  Google Scholar 

  39. Dong J, Hong Y, Shao Z, Liu Z (2010) Molecular cloning, purification, and characterization of a novel, acidic, pH-stable endoglucanase from Martelella mediterranea. J Microbiol 48:393–398

    Article  CAS  PubMed  Google Scholar 

  40. Lee YJ, Kim BK, Lee BH, Jo KI, Lee NK, Chung CH, Lee YC, Lee JW (2008) Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull. Bioresour Technol 99:378–386

    Article  CAS  PubMed  Google Scholar 

  41. Waghmare PR, Kshirsagar SD, Saratale RG, Govindwar SP, Saratale GD (2014) Production and characterization of cellulolytic enzymes by isolated Klebsiella sp. PRW-1 using agricultural waste biomass. Emir J Food Agric 26:44–59

    Article  Google Scholar 

  42. Chauhan PS, Sharma P, Puri N, Gupta N (2014) Purification and characterization of an alkali-thermostable β-mannanase from Bacillus nealsonii PN-11 and its application in manno-oligosaccharides preparation having prebiotic potential. Eur Food Res Technol 238(6):927–936

    Article  CAS  Google Scholar 

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Acknowledgements

This study is supported by DBT-Indo-Finland Project “ALGOMEG” (Project no. 7068).

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Author notes

  1. Heena Prakash and Prakram Singh Chauhan contributed equally.

Authors and Affiliations

  1. Department of Biological Sciences, College of Basic Sciences and Humanities, G B Pant University of Agricultural and Technology, Pantnagar, Uttarakhand, 263145, India

    Heena Prakash, Prakram Singh Chauhan, Thiyam General & A. K. Sharma

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  1. Heena Prakash
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  2. Prakram Singh Chauhan
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Correspondence to A. K. Sharma.

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Prakash, H., Chauhan, P.S., General, T. et al. Development of eco-friendly process for the production of bioethanol from banana peel using inhouse developed cocktail of thermo-alkali-stable depolymerizing enzymes. Bioprocess Biosyst Eng 41, 1003–1016 (2018). https://doi.org/10.1007/s00449-018-1930-3

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  • DOI: https://doi.org/10.1007/s00449-018-1930-3

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