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Biocatalysis of aromatic benzyl-propionate ester by different immobilized lipases

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Abstract

Benzyl propionate is an aromatic ester that possesses a fruity odor and is usually found in nature in the composition of some fruits such as plums and melons. This work aimed for the benzyl propionate synthesis by esterification using a new immobilized enzyme preparation with low-cost material from Candida antarctica (NS 88011) and three commercial immobilized lipases (Novozym 435, Lipozyme TL-IM and Lipozyme RM-IM). Novozym 435 had the best performance even when the solvent tert-butanol was absent of the reaction medium. Results from a 22 factorial design showed that an increase in the enzyme amount led to a higher conversion, even when the temperature was kept at the low value. Currently, no research had synthesized successfully benzyl propionate via esterification mediated by lipases; and we reached an ester conversion of ~ 44% after 24 h indicating that it is a promising route for benzyl propionate biotechnological production.

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Acknowledgements

The authors thank the financial support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).

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Authors and Affiliations

  1. Laboratório de Controle de Processos, Departamento Engenharia Química e Engenharia de Alimentos (EQA), Universidade Federal de Santa Catarina (UFSC), Campus Trindade, Cx. Postal 476, Florianópolis, SC, 88010-970, Brazil

    Amanda Gomes Almeida Sá, Alessandra Cristina de Meneses, Lindomar Alberto Lerin, Pedro Henrique Hermes de Araújo, Cláudia Sayer & Débora de Oliveira

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  1. Amanda Gomes Almeida Sá
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  2. Alessandra Cristina de Meneses
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  3. Lindomar Alberto Lerin
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  4. Pedro Henrique Hermes de Araújo
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  5. Cláudia Sayer
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  6. Débora de Oliveira
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Correspondence to Débora de Oliveira.

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Amanda Gomes Almeida Sá and Alessandra Cristina de Meneses contributed equally to this work.

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Sá, A.G.A., de Meneses, A.C., Lerin, L.A. et al. Biocatalysis of aromatic benzyl-propionate ester by different immobilized lipases. Bioprocess Biosyst Eng 41, 585–591 (2018). https://doi.org/10.1007/s00449-018-1893-4

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  • DOI: https://doi.org/10.1007/s00449-018-1893-4

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