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Effective pretreatment of dilute NaOH-soaked chestnut shell with glycerol–HClO4–water media: structural characterization, enzymatic saccharification, and ethanol fermentation

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Abstract

In this study, an effective pretreatment of dilute NaOH-soaked chestnut shell (CNS) with glycerol–HClO4–water (88.8:1.2:10, w/w/w) media at 130 °C for 30 min was successfully demonstrated. Results revealed that the combination pretreatment removed 66.0 % of lignin and 73.7 % of hemicellulose in untreated CNS. The changes in the structural features (crystallinity, morphology, and porosity) of the solid residue of CNS were characterized with Fourier transform infrared spectroscopy, fluorescent microscope, scanning electron microscopy, and X-ray diffraction. Biotransformation of glycerol–HClO4–water pretreated–NaOH-soaked CNS (50 g/L) with a cocktail of enzymes for 72 h, the reducing sugars and glucose were 39.7 and 33.4 g/L, respectively. Moreover, the recovered hydrolyzates containing 20 g/L glucose had no inhibitory effects on the ethanol-fermenting microorganism, and the ethanol production was 0.45 g/g glucose within 48 h. In conclusion, this combination pretreatment shows promise as pretreatment solvent for wheat straw, although the in-depth exploration of this subject is needed.

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References

  1. Vázquez G, Calvo M, Freire MS, González-Álvarez J, Antorrena G (2009) Chestnut shell as heavy metal adsorbent: optimization study of lead, copper and zinc cations removal. J Hazard Mater 172:1402–1414

    Article  Google Scholar 

  2. He YC, Liu F, Gong L, Di JH, Ding Y, Ma CL, Zhang DP, Tao ZC, Wang C, Yang B (2016) Enzymatic in situ saccharification of chestnut shell with high ionic liquid-tolerant cellulases from Galactomyces sp. CCZU11-1 in a biocompatible ionic liquid-cellulase media. Bioresour Technol 201:133–139

    Article  CAS  Google Scholar 

  3. Vázquez G, Mosquera O, Freire MS, Antorrena G, González-Álvarez J (2012) Alkaline pre-treatment of waste chestnut shell from a food industry to enhance cadmium, copper, lead and zinc ions removal. Chem Eng J 184:147–155

    Article  Google Scholar 

  4. Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol 96:673–686

    Article  CAS  Google Scholar 

  5. He YC, Liu F, Di JH, Ding Y, Zhu ZZ, Wu YQ, Chen L, Wang C, Xue YF, Chong GG, Ma CL (2016) Effective enzymatic saccharification of dilute NaOH extraction of chestnut shell pretreated by acidified aqueous ethylene glycol media. Ind Crop Prod 81:129–138

    Article  CAS  Google Scholar 

  6. He YC, Liu F, Gong L, Zhu ZZ, Ding Y, Wang C, Xue YF, Rui H, Tao ZC, Zhang DP, Ma CL (2015) Significantly improving enzymatic saccharification of high crystallinity index’s corn stover by combining ionic liquid [Bmim]Cl-HCl-water media with dilute NaOH pretreatment. Bioresour Technol 189:421–425

    Article  CAS  Google Scholar 

  7. Wen JL, Yuan TQ, Sun SL, Xu F, Sun RC (2014) Understanding the chemical transformations of lignin during ionic liquid pretreatment. Green Chem 16:181–190

    Article  CAS  Google Scholar 

  8. Moxley G, Gaspar AR, Higgins D, Xu H (2012) Structural changes of corn stover lignin during acid pretreatment. J Ind Microbiol Biotechnol 39:1289–1299

    Article  CAS  Google Scholar 

  9. Alvira P, Tomás-Pejó E, Ballesteros M, Negro MJ (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol 101:4851–4961

    Article  CAS  Google Scholar 

  10. Kim Y, Kreke T, Ko JK, Ladisch MR (2015) Hydrolysis-determining substrate characteristics in liquid hot water pretreated hardwood. Biotechnol Bioeng 112:677–687

    Article  CAS  Google Scholar 

  11. Himmel ME, Ding SY, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315:804–807

    Article  CAS  Google Scholar 

  12. Studer MH, DeMartini JD, Davis MF, Sykes RW, Davison B, Keller M, Tuskan GA, Wyman CE (2011) Lignin content in natural Populus variants affects sugar release. Proc Natl Acad Sci USA 108:6300–6305

    Article  CAS  Google Scholar 

  13. Yan Z, Li J, Chang S, Cui T, Jiang Y, Yu M, Zhang L, Zhao G, Qi P, Li S (2015) Lignin relocation contributed to the alkaline pretreatment efficiency of sweet sorghum bagasse. Fuel 158:152–158

    Article  CAS  Google Scholar 

  14. Li Q, He YC, Xian M, Jun G, Xu X, Yang JM, Li LZ (2009) Improving enzymatic hydrolysis of wheat straw using ionic liquid 1-ethyl-3-methyl imidazolium diethyl phosphate pretreatment. Bioresour Technol 100:3570–3575

    Article  CAS  Google Scholar 

  15. Choi WL, Park JY, Lee JP, Oh YK, Park YC, Kim JS, Park JM, Kim CH, Lee JS (2013) Optimization of NaOH-catalyzed steam pretreatment of empty fruit bunch. Biotechnol Biof 6:170

    Article  Google Scholar 

  16. He YC, Xia DQ, Ma CL, Gong L, Gong T, Wu MX, Zhang Y, Tang YJ, Xu JH, Liu YY (2013) Enzymatic saccharification of sugarcane bagasse by N-methylmorpholine-N-oxide-tolerant cellulase from a newly isolated Galactomyces sp. CCZU11-1. Bioresour Technol 135:18–22

    Article  CAS  Google Scholar 

  17. Hendriks ATWM, Zeeman G (2009) Pretreatments to enhance the digestibility of lignocellulosic biomass. Biotechnol Prog 100:10–18

    CAS  Google Scholar 

  18. He YC, Ding Y, Xue YF, Yang B, Liu F, Wang C, Zhu ZZ, Qing Q, Wu H, Zhu C, Tao ZC, Zhang DP (2015) Enhancement of enzymatic saccharification of corn stover with sequential Fenton pretreatment and dilute NaOH extraction. Bioresour Technol 193:324–330

    Article  CAS  Google Scholar 

  19. Xu J, Thomsen MH, Thomsen AB (2009) Enzymatic hydrolysis and fermentability of corn stover pretreated by lactic acid and/or acetic acid. J Biotechnol 139:300–305

    Article  CAS  Google Scholar 

  20. He YC, Liu F, Gong L, Lu T, Ding Y, Zhang DP, Qing Q, Zhang Y (2015) Improving enzymatic Hydrolysis of corn stover pretreated by ethylene glycol-perchloric acid-water mixture. Appl Biochem Biotechnol 175:1306–1317

    Article  CAS  Google Scholar 

  21. Xu J, Cheng JJ, Sharma-Shivappa RR, Burns JC (2010) Sodium hydroxide pretreatment of switchgrass for ethanol production. Energy Fuels 24:2113–2119

    Article  CAS  Google Scholar 

  22. da Silva GP, Mack M, Contiero J (2009) Glycerol: a promising and aboundant carbon source for industrial microbiology. Biotechnol Adv 27:30–39

    Article  Google Scholar 

  23. Demirbas A (1998) Aqueous glycerol delignification of wood chips and ground wood. Bioresour Technol 63:179–185

    Article  CAS  Google Scholar 

  24. O’Driscoll C (2007) Seeking a new role for glycerol. Biofuels Bioprod Bioref-Biofpr 1:7

    Google Scholar 

  25. Novo LP, Gurgel LVA, Marabezi K, da Silva Curvelo AA (2011) Delignification of sugarcane bagasse using glycerol-water mixtures to produce pulps for saccharification. Bioresour Technol 102:10040–10046

    Article  CAS  Google Scholar 

  26. Liu J, Takada R, Karita S, Watanabe T, Honda Y, Watanabe T (2010) Microwave-assisted pretreatment of recalcitrant softwood in aqueous glycerol. Bioresour Technol 101:9355–9360

    Article  CAS  Google Scholar 

  27. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory, Golden, CO, USA

    Google Scholar 

  28. Sambusiti C, Ficara E, Malpei F, Steyer JP, Carrère H (2013) Benefit of sodium hydroxide pretreatment of ensiled sorghum forage on the anaerobic reactor stability and methane production. Bioresour Technol 144:149–155

    Article  CAS  Google Scholar 

  29. Li Q, Jiang XL, He YC, Li LZ, Xian M, Yang JM (2010) Evaluation of the biocompatible ionic liquid 1-methyl-3-methylimidazolium dimethylphosphate pretreatment of corn cob for improved saccharification. Appl Microbiol Biotechnol 87:117–126

    Article  CAS  Google Scholar 

  30. Qing Q, Hu R, He YC, Zhang Y, Wang LQ (2014) Investigation of a novel acid-catalyzed ionic liquid pretreatment method to improve biomass enzymatic hydrolysis conversion. Appl Miocrob Biotechnol 98:5275–5286

    Article  CAS  Google Scholar 

  31. Qin YM, Tao H, Liu YY, Wang YD, Zhang JR, Tang AX (2013) A novel non-hydrolytic protein from Pseudomonas oryzihabitans enhances the enzymatic hydrolysis of cellulose. J Biotechnol 168:24–31

    Article  CAS  Google Scholar 

  32. Ko JK, Ximenes E, Kim Y, Ladisch MR (2015) Adsorption of enzyme onto lignins of liquid hot water pretreated hardwoods. Biotechnol Bioeng 112:447–456

    Article  CAS  Google Scholar 

  33. Sun FB, Chen HZ (2008) Enhanced enzymatic hydrolysis of wheat straw by aqueous glycerol pretreatment. Bioresour Technol 99:6156–6161

    Article  CAS  Google Scholar 

  34. Zhu HX, Cao XJ, He YC, Kong QP, He H, Wang J (2015) Removal of Cu2+ from aqueous solutions by the novel modified bagasse pulp cellulose: kinetics, isotherm and mechanism. Carbohydr Polym 129:115–126

    Article  CAS  Google Scholar 

  35. Maurelli L, Ionata E, La Cara F, Morana A (2013) Chestnut shell as unexploited source of fermentable sugars: effect of different pretreatment methods on enzymatic saccharification. Appl Biochem Biotechnol 170:1104–1118

    Article  CAS  Google Scholar 

  36. Hundt M, Schnitzlein K, Schnitzlein MG (2013) Alkaline polyol pulping and enzymatic hydrolysis of hardwood: effect of pulping severity and pulp composition on cellulase activity and overall sugar yield. Bioresour Technol 136:672–679

    Article  CAS  Google Scholar 

  37. Hundt M, Schnitzlein K, Schnitzlein MG (2013) Alkaline polyol pulping and enzymatic hydrolysis of softwood: effect of pulping severity and pulp properties on cellulase activity and overall sugar yield. Bioresour Technol 134:307–315

    Article  CAS  Google Scholar 

  38. Martin C, Puls J, Saake B, Schreiber A (2011) Effect of glycerol preatreatment on component recovery and enzymatic hydrolysis of sugarcane bagasse. Cell Chem Technol 45:487–494

    CAS  Google Scholar 

  39. Romani A, Ruiz HA, Pereira FB, Domingues L, Teixeira JA (2013) Fractionation of Eucalyptus globulus wood by glycerol-water pretreatment: optimization and modeling. Ind Eng Chem Res 52:14342–14352

    Article  CAS  Google Scholar 

  40. Sun FF, Wang L, Hong J, Ren J, Du F, Hu J, Zhang Z, Zhou B (2015) The impact of glycerol organosolv pretreatment on the chemistry and enzymatic hydrolyzability of wheat straw. Bioresour Technol 187:354–361

    Article  CAS  Google Scholar 

  41. Jachmanián I, Martínez N, Segura N (2015) Enhancing the enzymatic synthesis of alkyl esters by coupling transesterification to an efficient glycerol separation system. Fuel 153:13–18

    Article  Google Scholar 

  42. Li AT, Ngo TPN, Yan JY, Tian KY, Li Z (2012) Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease. Bioresour Technol 114:725–729

    Article  CAS  Google Scholar 

  43. Delgado R, Rosas JG, Gómez N, Martínez O, Sanchez ME, Cara J (2013) Energy valorisation of crude glycerol and corn straw by means of slow co-pyrolysis: production and characterisation of gas, char and bio-oil. Fuel 112:31–37

    Article  CAS  Google Scholar 

  44. Kang HK, Kim D (2011) Efficient bioconversion of rice straw to ethanol with TiO2/UV pretreatment. Bioprocess Biosys Eng 35:43–48

    Article  Google Scholar 

  45. Kim SB, Lee JH, Oh KK, Lee SJ, Lee JY, Kim JS, Kim SW (2011) Dilute acid pretreatment of barley straw and its saccharification and fermentation. Biotechnol Bioprocess Eng 16:725–732

    Article  CAS  Google Scholar 

  46. Xu F, Shi YC, Wu X, Theerarattananoon K, Staggenborg S, Wang D (2011) Sulfuric acid pretreatment and enzymatic hydrolysis of photoperiod sensitive sorghum for ethanol production. Bioprocess Biosys Eng 34:485–492

    Article  CAS  Google Scholar 

  47. Zhang J, Wang M, Gao M, Fang X, Yano S, Qin S, Xia R (2013) Efficient acetone–butanol–ethanol production from corncob with a new pretreatment technology—wet disk milling. Bioenerg Res 6:35–43

    Article  Google Scholar 

  48. Unrean P, Khajeeram S (2015) Model-based optimization of Scheffersomyces stipitis and Saccharomyces cerevisiae co-culture for efficient lignocellulosic ethanol production. Bioresour Bioprocess 2:41

    Article  Google Scholar 

  49. Plácido J, Capareda S (2014) Analysis of alkali ultrasonication pretreatment in bioethanol production from cotton gin trash using FT-IR spectroscopy and principal component analysis. Bioresour Bioprocess 1:23

    Article  Google Scholar 

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 21102011), the Natural Science Foundation of Jiangsu Province (No. BK20141172), the Open Project Program of the State Key Laboratory of Bioreactor Engineering, and the Key Laboratory of Guangxi Biorenery (No. GXBF11-03).

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Authors and Affiliations

  1. Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China

    Yu-Cai He, Feng Liu, Jun-Hua Di, Yun Ding, Da-Zhou Gao, Dan-Ping Zhang, Zhi-Cheng Tao, Gang-Gang Chong, Mei-Zi Huang & Cui-Luan Ma

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  1. Yu-Cai He
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He, YC., Liu, F., Di, JH. et al. Effective pretreatment of dilute NaOH-soaked chestnut shell with glycerol–HClO4–water media: structural characterization, enzymatic saccharification, and ethanol fermentation. Bioprocess Biosyst Eng 39, 533–543 (2016). https://doi.org/10.1007/s00449-015-1535-z

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