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Amperometric hydrogen peroxide biosensor based on covalently immobilizing thionine as a mediator

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Abstract

A novel amperometric hydrogen peroxide biosensor based on the immobilization of hemoglobin on the 2,6-pyridinedicarboxylic acid (PDC) polymer, thionine and nano-Au was successfully fabricated. In this strategy, PDC polymer acted as the matrices to covalently immobilize the thionine, and then hemoglobin was successfully adsorbed on the nano-Au which was electro-deposited on to thionine modified electrode surface. The preparation process of modified electrode was characterized with electrochemical impedance spectroscopy and atomic force microscope. The analytical performance of proposed biosensor toward H2O2 was investigated by cyclic voltammetry and chronoamperometry. The resulted biosensor exhibited fast amperometric response (within 6 s) to H2O2, and linear range was from 9.1 μM to 5.0 mM with the detection limit of 2.6 μM (S/N = 3). The apparent Michaelis–Menten constant (K appM ) was evaluated to be 3.2 mM. Furthermore, the resulted biosensor showed good stability and reproducibility.

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Acknowledgments

Financial support of this work was provided by the National Natural Science Foundation of China (20675064), the Natural Science Foundation of Chongqing City (CSTC-2004BB4149, 2005BB4100), China and High Technology Project Foundation of Southwest University (XSGX 02), China.

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Authors and Affiliations

  1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, China

    Liping Ma, Ruo Yuan, Yaqin Chai, Shihong Chen & Shujuan Ling

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  1. Liping Ma
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  2. Ruo Yuan
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  3. Yaqin Chai
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  4. Shihong Chen
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  5. Shujuan Ling
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Correspondence to Ruo Yuan.

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Ma, L., Yuan, R., Chai, Y. et al. Amperometric hydrogen peroxide biosensor based on covalently immobilizing thionine as a mediator. Bioprocess Biosyst Eng 32, 537–544 (2009). https://doi.org/10.1007/s00449-008-0275-8

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  • DOI: https://doi.org/10.1007/s00449-008-0275-8

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