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Induction of human umbilical cord mesenchymal stem cells into tissue-forming cells in a murine model: implications for pelvic floor reconstruction

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Abstract

HUMSCs were isolated, differentiated and characterized in vitro. Both HUMSCs and smooth muscle cells differentiated from HUMSCs were used to fabricate tissue-engineered fascia equivalents. Forty-eight mature female Sprague Dawley rats were randomly assigned to four groups: group A (GynemeshTMPS, n = 12), group B (GynemeshTMPS + HUMSCs; n = 12), group C (GynemeshTMPS + smooth muscle cells differentiated from HUMSCs; n = 12) and group D (GynemeshTMPS + HUMSCs + smooth muscle cells differentiated from HUMSCs; n = 12). The posterior vaginal wall was incised from the introitus and the mesh was then implanted. Three implants of each type were tested at 1, 4, 8 and 12 weeks. Fibrotic remodeling, inflammation, vascularization and tissue regeneration were histologically assessed. The levels of type I and type III collagen were determined. There was no difference in fibrotic remodeling between cell-seeded and unseeded meshes at any time (p > 0.05). At 12 weeks, there did not appear to be fewer inflammatory cells around the filament bundles in the mesh with cells compared with the mesh alone (P > 0.05). Group D showed a trend toward better vascularization at 12 weeks compared with group A (P < 0.05). Twelve weeks after implantation, a thin layer of new tissue growth covered the unseeded scaffold and a thicker layer covered the cell-seeded scaffold (P < 0.05). No significant difference in the ratio of collagen type I/III could be detected among the different groups after 12 weeks (P > 0.05). HUMSCs with differentiated smooth muscle cells might have a potential role in fascia tissue engineering to repair POP in the future.

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Acknowledgements

We thank the animal care staff at PUMCH for their assistance in managing the husbandry of the rats used in this study. We are grateful to Mrs. Xiao-yan Cheng for her excellent assistance during this experiment.

Funding

This study received financial support from the National Natural Science Foundation of China (No. 81571421) and the Postdoctoral Foundation of China (2016 M600263).

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Author notes

  1. Jing Ding and Qin Han contributed equally to this work.

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China

    Jing Ding, Mou Deng, Xiao-chen Song, Chun Chen, Fang-fang Ai & Lan Zhu

  2. Department of Gynecology, Harbin Medical University Cancer Hospital, No. 150 Haping Road, Nangang District, Harbin, Heilongjiang Province, 150040, China

    Jing Ding

  3. Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Tissue Engineering Center of Chinese Academy of Medical Sciences, Beijing, China

    Qin Han & Robert Chun-hua Zhao

  4. Beijing, China

    Lan Zhu & Robert Chun-hua Zhao

  5. Center of Translational Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

    Robert Chun-hua Zhao

Authors
  1. Jing Ding
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  2. Qin Han
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  3. Mou Deng
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  4. Xiao-chen Song
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  6. Fang-fang Ai
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  7. Lan Zhu
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  8. Robert Chun-hua Zhao
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Contributions

J Ding: Protocol development, data collection, data analysis and manuscript writing.

Q Han: Protocol development, data collection, data analysis and manuscript writing.

M Deng: Analysis of tissue response in the animal model.

X-C Song: Data collection.

C Chen: Manuscript writing.

F-f Ai: Data analysis.

L Zhu: Protocol development.

R Chun-hua Zhao: Protocol development.

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Ding, J., Han, Q., Deng, M. et al. Induction of human umbilical cord mesenchymal stem cells into tissue-forming cells in a murine model: implications for pelvic floor reconstruction. Cell Tissue Res 372, 535–547 (2018). https://doi.org/10.1007/s00441-017-2781-y

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  • DOI: https://doi.org/10.1007/s00441-017-2781-y

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