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Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells

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Abstract

A tissue-engineered mesh fabricated with adipose-derived mesenchymal stem cells (AD-MSCs) cultured on a silk fibroin scaffold is evaluated for use in female pelvic reconstruction. Thirty-five female Sprague Dawley rats were divided into four groups. Group A (n = 10) were implanted with polypropylene meshes, Group B (n = 10) with silk fibroin scaffolds and Group C (n = 10) with tissue-engineered meshes. Group D (n = 5) acted as the tissue control. The tissue-engineered mesh was produced as follows. AD-MSCs were obtained from adipose tissue of rats designated to Group C. The cells were seeded onto a silk fibroin scaffold, cultured and then observed by scanning electron microscopy (SEM). Histological studies of these meshes were performed at 4 and 12 weeks after implantation and mechanical testing was carried out on all groups before implantation and at 12 weeks after implantation. AD-MSCs displayed fibroblast-like shapes and were able to differentiate into adipocytes or fibroblasts. SEM observation showed that AD-MSCs proliferated and secreted a matrix onto the silk fibroin scaffolds. After implantation of the scaffolds into rats, histological analysis revealed better organized newly formed tissue in Group C than in controls. Group C also had a similar failure force (2.67 ± 0.15 vs 2.33 ± 0.38 N) and a higher Young’s modulus (2.99 ± 0.19 vs 1.68 ± 0.20 MPa) than a normal vaginal wall, indicating the potential of this tissue-engineered approach. AD-MSCs were validated as seed cells for tissue engineering. The silk fibroin scaffold thus shows promise for application with AD-MSCs in the fabrication of tissue-engineered mesh with good biocompatibility and appropriate mechanical properties for pelvic floor reconstruction.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Peking University People’s Hospital, 11 Xizhimen South Street, , Xicheng District, Beijing, 100044, People’s Republic of China

    Qi Li, Jianliu Wang, Bing Xie & Lihui Wei

  2. School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, People’s Republic of China

    Haifeng Liu

Authors
  1. Qi Li
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  2. Jianliu Wang
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  3. Haifeng Liu
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  4. Bing Xie
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  5. Lihui Wei
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Corresponding author

Correspondence to Jianliu Wang.

Additional information

This research was supported by the Beijing Natural Science Foundation (no. 7122194) and Peking University (“985 Project”, no. 51917715021801203).

The authors declare no financial or other conflicts of interest.

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Li, Q., Wang, J., Liu, H. et al. Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells. Cell Tissue Res 354, 471–480 (2013). https://doi.org/10.1007/s00441-013-1719-2

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  • DOI: https://doi.org/10.1007/s00441-013-1719-2

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