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Genome-wide identification and expression analysis of stress-associated proteins (SAPs) containing A20/AN1 zinc finger in cotton

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Abstract

Stress-associated proteins (SAPs) containing the A20/AN1 zinc-finger domain play important roles in response to both biotic and abiotic stresses in plants. Nevertheless, few studies have focused on the SAP gene family in cotton. To explore the distributions and expression patterns of these genes, we performed genome-wide identification and characterization of SAPs in tetraploid Gossypium hirsutum L. TM-1 (AD1). A total of 37 genes encoding SAPs were identified, 36 of which were duplicated in the A and D sub-genomes. The analysis of gene architectures and conserved protein motifs revealed that nearly all A20–AN1-type SAPs were intron-free, whereas AN1–AN1-type SAPs contained one intron. The cis-elements of the SAP promoters were studied, as were the expression levels of cotton SAP genes under different stresses based on RNA-seq data and validated by qRT-PCR. Most cotton SAP genes were induced by multiple stresses and phytohormones, particularly salt stress, indicating that SAP genes may play important roles in cotton’s response to unfavorable environmental changes. Among these identified SAPs, the expression of GhSAP17A/D is suppressed in cotton response to Vertillium dahliae, and the GhSAP17A/D-silenced cotton exhibits more resistance to V. dahliae. This study provides insight into the evolution of SAP genes in upland cotton and may aid in efforts at further functional identification of A20/AN1-type proteins and cotton’s response to different stresses.

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Abbreviations

SAP:

Stress-associated protein

HMM:

Hidden markov model

qRT-PCR:

Quantitative real-time PCR

ABA:

Abscisic acid

ETH:

Ethylene

MeJA:

Methyl jasmonate

PEG:

Polyethylene glycol

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Acknowledgments

This work was financially supported by the Ministry of Agriculture of China (Grant No. 2016ZX08009-003-002), State Key Laboratory of Cotton Biology Open Fund (CB2015A31), and Henan Provincial Educational Department Foundation of China (15A180028, 15A180029).

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Authors and Affiliations

  1. State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, People’s Republic of China

    Wei Gao, Lu Long, Xinquan Tian, Huili Liu, Hui Zhang, Fuchun Xu & Chunpeng Song

  2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, Henan, People’s Republic of China

    Jingjing Jin

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  1. Wei Gao
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  2. Lu Long
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  3. Xinquan Tian
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  6. Hui Zhang
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  8. Chunpeng Song
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Correspondence to Chunpeng Song.

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Communicated by S. Hohmann.

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Gao, W., Long, L., Tian, X. et al. Genome-wide identification and expression analysis of stress-associated proteins (SAPs) containing A20/AN1 zinc finger in cotton. Mol Genet Genomics 291, 2199–2213 (2016). https://doi.org/10.1007/s00438-016-1252-6

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