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Molecular characterization of a cold-induced plasma membrane protein gene from wheat

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Abstract

As a means to study the function of plasma membrane proteins during cold acclimation, we have isolated a cDNA clone for wpi6 which encodes a putative plasma membrane protein from cold-acclimated winter wheat. The wpi6 gene encodes a putative 5.9 kDa polypeptide with two predicted membrane-spanning domains, the sequence of which shows high sequence similarity with BLT101-family proteins from plants and yeast. Strong induction of wpi6 mRNA was observed during an early stage of cold acclimation in root and shoot tissues of both winter and spring wheat cultivars. In contrast to blt101 in barley, wpi6 mRNA was also induced by drought and salinity stresses, and exogenous application of ABA. Expression of wpi6 in a Δpmp3 mutant of Saccharomyces cerevisiae, which is disturbed in plasma membrane potential due to the lack of a BLT101-family protein, partially complemented NaCl sensitivity of the mutant. Transient expression analysis of a WPI6::GFP fusion protein in onion epidermal cells revealed that WPI6 is localized in the plasma membrane. Taken together, these data suggested that WPI6 may have a protective role in maintaining plasma membrane function during cold acclimation in wheat.

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Acknowledgements

We would like to thank Drs. Yasuo Niwa (University of Shizuoka) and Takashi Okamoto (Tokyo Metropolitan University) for kindly providing the sGFP(S65T) and the DsRed::HDEL plasmids, respectively. We also would like to thank Dr. Taihachi Kawahara (Kyoto University) for kindly providing seeds of diploid and tetraploid wheat species. This work was supported by a grant from MAFF (BioDesign 1207) to R.I. and Cooperating System for Supporting Priority Research, Japan Science and Technology Cooperation for R.I.

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Authors and Affiliations

  1. Winter Stress Laboratory, National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, 062-8555, Toyohira-ku, Sapporo, Japan

    Ryozo Imai, Michiya Koike, Keita Sutoh, Akira Kawakami & Kiyoharu Oono

  2. Hokkaido Green-Bio Institute, 069-1317, Naganuma, Hokkaido, Japan

    Michiya Koike & Atsushi Torada

Authors
  1. Ryozo Imai
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  2. Michiya Koike
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  3. Keita Sutoh
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  4. Akira Kawakami
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  5. Atsushi Torada
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  6. Kiyoharu Oono
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Corresponding author

Correspondence to Ryozo Imai.

Additional information

Communicated by R. Hagemann

The nucleotide sequence data for wpi6 have been recorded in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession numbers AB030210 (cDNA) and AB221353 (genomic DNA).

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Imai, R., Koike, M., Sutoh, K. et al. Molecular characterization of a cold-induced plasma membrane protein gene from wheat. Mol Genet Genomics 274, 445–453 (2005). https://doi.org/10.1007/s00438-005-0050-3

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  • DOI: https://doi.org/10.1007/s00438-005-0050-3

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