Abstract
Five newly obtained nuclear ribosomal transcription unit (rTU) sequences from Echinostomatidae and Echinochasmidae are presented. The inter- and intrafamilial relationships of these and other families in the suborder Echinostomata are also analyzed. The sequences obtained are the complete rTU of Artyfechinostomum malayanum (9,499 bp), the near-complete rTU of Hypoderaeum conoideum (8,076 bp), and the coding regions (from 5’-terminus of 18S to 3’-terminus of 28S rRNA gene) in Echinostoma revolutum (6,856 bp), Echinostoma miyagawai (6,854 bp), and Echinochasmus japonicus (7,150 bp). Except for the longer first internal transcribed spacer (ITS1) in Echinochasmus japonicus, all genes and spacers were almost identical in length. Comprehensive maximum-likelihood phylogenies were constructed using the PhyML software package. The datasets were either the concatenated 28S + 18S rDNA sequences (5.7–5.8 kb) from 60 complete rTUs of 19 families or complete 28S sequences only (about 3.8–3.9 kb) from 70 strains or species of 22 families. The phylogenetic trees confirmed Echinostomatoidea as monophyletic. Furthermore, a detailed phylogeny constructed from alignments of 169 28S D1−D3 rDNA sequences (1.1–1.3 kb) from 98 species of 50 genera of 10 families, including 154 echinostomatoid sequences (85 species/42 genera), clearly indicated known generic relationships within Echinostomatidae and Echinochasmidae and relationships of families within Echinostomata and several other suborders. Within Echinostomatidae, Echinostoma, Artyfechinostomum, and Hypoderaeum appeared as monophyletic, while Echinochasmus (Echinochasmidae) was polyphyletic. The Echinochasmidae are a sister group to the Psilostomidae. The datasets provided here will be useful for taxonomic reappraisal as well as studies of evolutionary and population genetics in the superfamily Echinostomatoidea, the sole superfamily in the suborder Echinostomata.
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Data availability
The nucleotide sequences obtained in this study have been submitted to GenBank and were assigned the accession numbers OR509026–OR509030 for the ribosomal transcription units of Artyfechinostomum malayanum, Echinostoma miyagawai, Echinostoma revolutum, Hypoderaeum conoideum, and Echinochasmus japonicus, respectively. The GenBank numbers of the partial sequences of the 28S rRNA gene are OR532444 for Echinochasmus japonicus, OR532445 for Echinochasmus perfoliatus, and OR532446 for Patagifer bilobus.
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This work was funded by the Supporting Program for the Principal Research Scientists from the Vietnam Academy of Science and Technology (VAST) to Prof. Dr. Thanh Hoa Le (Grant No: NVCC08-05/22–23).
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Thanh Hoa Le conceived and designed the study, analyzed the final data, prepared figures and tables, and wrote the first draft of the manuscript. Linh Thi Khanh Pham, Khue Thi Nguyen, Huong Thi Thanh Doan, and Dong Van Quyen conducted laboratory work and performed sequence analyses. Linh Thi Khanh Pham is a PhD student at the University of Science and Technology of Hanoi (Vietnam). Weerachai Saijuntha collected, molecularly identified, and provided specimens. David Blair reviewed all the data and drafts and finalized the MS. Weerachai Saijuntha, David Blair, and Thanh Hoa Le completed and approved the manuscript. The results were critically appraised and interpreted by all authors, who also read and approved the final manuscript version.
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Le, T.H., Pham, L.T.K., Van Quyen, D. et al. The ribosomal transcription units of five echinostomes and their taxonomic implications for the suborder Echinostomata (Trematoda: Platyhelminthes). Parasitol Res 123, 103 (2024). https://doi.org/10.1007/s00436-023-08110-z
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DOI: https://doi.org/10.1007/s00436-023-08110-z