Abstract
The Spirometra erinacei casein kinase I (SeCKI) gene was cloned and expressed in Escherichia coli, and its characteristics were investigated in this study. The recombinant SeCP protein (rSeCKI) was purified. The vaccination of mice with rSeCKI induced the Th1/Th2-mixed type of immune response with Th2 predominant (high levels of IgG1). Western blotting analysis showed that rSeCP was recognized by the sera of plerocercoid-infected mice, and anti-rSeCP serum recognized the native SeCP protein of plerocercoid crude antigens. Transcription and expression of SeCP was observed at the plerocercoid and adult stages of S. erinacei. Immunolocalization identified SeCKI in the tegument and parenchymal tissues of plerocercoids and in the teguments of adults. SeCKI appeared to be essential indispensable for the S. erinacei development and survival in host, but its biological functions need to be further investigated.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 81172612, 81501768), the Major Project of Henan Medical Science and Technology (No.201401001), and the National College Students Innovation Training Program of China (No.201510459069).
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All of the animal experiments were approved by The Life Science Ethics Committee of Zhengzhou University.
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Liu, L.N., Wang, H., Jiang, P. et al. Molecular cloning and characterization of a Spirometra erinacei casein kinase I. Parasitol Res 114, 4737–4742 (2015). https://doi.org/10.1007/s00436-015-4758-7
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DOI: https://doi.org/10.1007/s00436-015-4758-7