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Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana

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Abstract

We conducted a study of serodiagnosis of experimental sparganum infections of mice and human sparganosis by enzyme-linked immunosorbent assay (ELISA) using excretory–secretory (ES) antigens of Spirometra mansoni spargana and compared the sensitivity and specificity of crude and ES antigens for detecting the specific anti-sparganum IgG antibodies. By crude antigen ELISA and ES antigen ELISA, anti-sparganum IgG was detected in all of 30 serum samples of the infected mice; no cross-reactions were observed in serum samples of the mice infected with Trichinella spiralis, Schistosoma japanicum, Toxoplasma gondii, and normal mice. Anti-sparganum IgG was detected by ES antigen ELISA in sera of mice infected with one, two, four, six, and eight spargana at 3 weeks post-infection (wpi), with a detection rate of 100%, and lasted to 18 wpi when the experiment was ended. The difference in anti-sparganum antibody levels among five groups of the infected mice was statistically significant (F = 245.296, p < 0.05); the antibody levels were correlated with infecting doses of spargana (r = 0.323, p < 0.05). The sensitivity of both ELISA in detecting the serum samples of patients with sparganosis was 100% (20/20), but 96.72% (59/61) of specificity of ES antigen ELISA in detecting serum samples of patients with cysticercosis, echinococcosis, paragonimiosis, clonorchiosis, and schistosomiasis, and healthy persons was significantly greater than 72.13% (44/61) of crude antigen ELISA (χ 2 = 14.027, p < 0.05). Our finding indicates that ELISA using ES antigens of S. mansoni spargana may be applied to the specific early serodiagnosis of sparganosis.

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Acknowledgments

This work was supported by grants from Henan Major Public Research Project (No. 2008-145) and Henan Medical Science and Technology (No. 201003006).

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Authors and Affiliations

  1. Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, 450052, People’s Republic of China

    Jing Cui, Nan Li, Zhong Quan Wang & Peng Jiang

  2. Department of Helminthiasis, Henan Center for Disease Control and Prevention, Zhengzhou, People’s Republic of China

    Xi Meng Lin

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  1. Jing Cui
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  2. Nan Li
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  3. Zhong Quan Wang
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  4. Peng Jiang
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Correspondence to Zhong Quan Wang.

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Cui, J., Li, N., Wang, Z.Q. et al. Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana. Parasitol Res 108, 1551–1556 (2011). https://doi.org/10.1007/s00436-010-2206-2

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  • DOI: https://doi.org/10.1007/s00436-010-2206-2

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