Abstract
The Spirometra mansoni antigenic polypeptide (SmAP) gene was expressed in Escherichia coli, and its characteristics and value as an antigen for the serodiagnosis of sparganosis were investigated. The recombinant SmAP protein (rSmAP) has the molecular weight of 28.7 kDa. On Western blotting analysis, the rSmAP strongly reacted with the sera of mice infected with spargana, but not with normal sera; the anti-rSmAP serum obviously recognized the 28.7-kDa band in the crude antigens and excretory–secretory (ES) antigens of spargana. The immunofluorescence test (IFT) results showed that the positive staining was observed at different stages of spargana from the infected frogs and mice, but not adult worm of S. mansoni. An immunolocalization analysis identified SmAP in the teguments and parenchymal tissues of spargana. ELISA with rSmAP antigen or sparganum ES antigens were evaluated for the serodiagnosis of sparganosis. The results showed that the sensitivity of rSmAP-ELISA and ES-ELISA was 83.3 % (25/30) and 100 % (30/30), respectively, for the detection of anti-sparganum IgG antibodies in sera of the experimentally infected mice (P > 0.05), the specificities of both ELISA were 100 % (67/67). It is suggested that the rSmAP might be a potential candidate antigen for serodiagnosis of sparganosis.
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References
Anantaphruti MT, Nawa Y, Vanvanitchai Y (2011) Human sparganosis in Thailand: an overview. Acta Trop 1183:171–176. doi:10.1016/j.actatropica.2011.03.011
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Branco LM et al (2008) Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance. Virol J 5:74. doi:10.1186/1743-422X-5-74
Cui J, Li N, Wang ZQ, Jiang P, Lin XM (2011a) Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana. Parasitol Res 1086:1551–1556. doi:10.1007/s00436-010-2206-2
Cui J, Lin XM, Zhang HW, Xu BL, Wang ZQ (2011b) Sparganosis, Henan Province, central China. Emerg Infect Dis 171:146–147. doi:10.3201/eid1701.101095
Cui J, Ren HJ, Liu RD, Wang L, Zhang ZF, Wang ZQ (2013) Phage-displayed specific polypeptide antigens induce significant protective immunity against Trichinella spiralis infection in BALB/c mice. Vaccine 318:1171–1177. doi:10.1016/j.vaccine.2012.12.070
Fukushima T, Yamane Y (1999) How does the sparganosis occur? Parasitol Today 153:124
Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory. America Patent
Hu DD, Cui J, Wang L, Liu LN, Wei T, Wang ZQ (2013) Immunoproteomic analysis of the excretory-secretory proteins from Spirometra mansoni sparganum. Iranian J Parasitol 83:408–416
Hu DD et al (2014) Identification of early diagnostic antigens from Spirometra mansoni sparganum soluble proteins using immunoproteomics. Southeast Asian J Trop Med Public Health 453:576–583
Kim JH, Kim YJ, Sohn WM, Bae YM, Hong ST, Choi MH (2009) Differential protein expression in Spirometra erinacei according to its development in its final host. Parasitol Res 1056:1549–1556. doi:10.1007/s00436-009-1585-8
Kong Y, Chung YB, Cho SY, Kang SY (1994) Cleavage of immunoglobulin G by excretory-secretory cathepsin S-like protease of Spirometra mansoni plerocercoid. Parasitology 109(Pt 5):611–621
Liu DW, Liu DJ, Leng SY, Liu SX (1999) Molecular cloning of a cDNA encoding an antigenic polypeptide containing repeat unit of Spirometra erinaceieuropaei plerocercoid. Chine J Parasitol Parasitic Dis 171:39–42
Magnino S et al (2009) Biological risks associated with consumption of reptile products. Int J Food Microbiol 1343:163–175. doi:10.1016/j.ijfoodmicro.2009.07.001
Murata K et al (2007) Difficulty in diagnosing a case with apparent sequel cerebral sparganosis. Surg Neurol 674:409–411, discussion 412
Nishiyama T, Ide T, Himes SR Jr, Ishizaka S, Araki T (1994) Immunodiagnosis of human sparganosis mansoni by micro-chemiluminescence enzyme-linked immunosorbent assay. Trans R Soc Trop Med Hyg 886:663–665
Nithiuthai S, Anantaphruti MT, Waikagul J, Gajadhar A (2004) Waterborne zoonotic helminthiases. Vet Parasitol 1261–2:167–193
Rahman SM, Kim JH, Hong ST, Choi MH (2014) Diagnostic efficacy of a recombinant cysteine protease of Spirometra erinacei larvae for serodiagnosis of sparganosis. Korean J Parasitol 521:41–46. doi:10.3347/kjp.2014.52.1.41
Roberts LS, Schmidt GD, Janovy J (2009) Gerald D. Schmidt & Larry S. Roberts’ Foundations of Parasitology, 8th edn. McGraw-Hill Higher Education, Boston
Wang SW, Wang ZQ, Cui J (2011) Protein change of intestinal epithelial cells induced in vitro by Trichinella spiralis infective larvae. Parasitol Res 1083:593–599. doi:10.1007/s00436-010-2102-9
Wang B, Wang ZQ, Jin J, Ren HJ, Liu LN, Cui J (2013) Cloning, expression and characterization of a Trichinella spiralis serine protease gene encoding a 35.5 kDa protein. Exp Parasitol 1342:148–154. doi:10.1016/j.exppara.2013.03.004
Zhang YL, Wang ZQ, Li LG, Cui J (2013) Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB/c mice. Parasit Vectors 61:246. doi:10.1186/1756-3305-6-246
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This work was supported by the National Natural Science Foundation of China (No. 81172612).
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Cui, J., Wei, T., Liu, L.N. et al. Molecular characterization of a Spirometra mansoni antigenic polypeptide gene encoding a 28.7 kDa protein. Parasitol Res 113, 3511–3516 (2014). https://doi.org/10.1007/s00436-014-4065-8
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DOI: https://doi.org/10.1007/s00436-014-4065-8