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Fas/FasL-dependent apoptosis of hepatocytes induced in rat and patients with Clonorchis sinensis infection

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Abstract

To investigate the injury and pathogenesis of Clonorchis sinensis (C. sinensis) to hepatocytes, the liver samples from Wistar rats and patients with C. sinensis infection were examined. The typical histopathological findings of clonorchiasis were observed in rats 4 to 12 weeks postinfection, and majority hepatocytes exhibited hydropic degeneration, even some hepatocytes showed densely condensed nuclei suggesting apoptosis in liver tissue. Apoptosis was found around the central vein or portal areas of liver tissue in rat infected with C. sinensis by transferase uridyl nick end labeling (TUNEL) assay. Compared with normal control, TUNEL-positive cells in liver tissue increase from 4 to 12 weeks postinfection with the peak at 8 weeks. Furthermore, the expression of mRNA and protein of Fas, FasL, and caspase-3 was stronger in infected group than normal control using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. Autopsy specimens from four patients infected with C. sinensis have the same findings detected by histopathology, TUNEL, and immunohistochemistry. These findings suggest that the C. sinensis can stimulate both hepatocytic apoptosis and hydropic degeneration that may be responsible for relationship between clinical manifestations and liver lesions in patients with clonorchiasis. These data also indicate a role for Fas/FasL-mediated pathway in the apoptosis that occurs in response to C. sinensis infection.

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Acknowledgments

This work was supported partly with grants from National Natural Science Foundation of China (J0730858), Outstanding Youth Foundation of Heilongjiang Province (JC04-05), Foundation of Heilongjiang Provincial Health Bureau (2005-37) and Youth Foundation of Harbin Medical University (060026). The experiments comply with the current laws of the country in which the experiments were performed.

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Authors and Affiliations

  1. Department of Microbiology and Parasitology, Harbin Medical University, 157th Baojian Road, Nangang District, Harbin, 150081, People’s Republic of China

    Xiaoli Zhang, Rong Da, Yunxia Dong, Wuqi Song, Xiaobei Chen, Hong Ling, Yang Che, Yihong Li & Fengmin Zhang

  2. Pathogenic Biological, Bio-pharmacological Key-Laboratory, Harbin, Heilongjiang Province, 150081, People’s Republic of China

    Fengmin Zhang

  3. Department of Pathology and Forensic Medicine, Harbin Medical University, 157th Baojian Road, Nangang District, Harbin, 150081, People’s Republic of China

    Zhanfeng Jin & Qi Huang

Authors
  1. Xiaoli Zhang
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  2. Zhanfeng Jin
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  3. Rong Da
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  4. Yunxia Dong
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  5. Wuqi Song
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  6. Xiaobei Chen
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  7. Qi Huang
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  8. Hong Ling
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  9. Yang Che
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  10. Yihong Li
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  11. Fengmin Zhang
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Corresponding author

Correspondence to Fengmin Zhang.

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Drs. Xiaoli Zhang and Zhanfeng Jin contributed equally to this work.

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Zhang, X., Jin, Z., Da, R. et al. Fas/FasL-dependent apoptosis of hepatocytes induced in rat and patients with Clonorchis sinensis infection. Parasitol Res 103, 393–399 (2008). https://doi.org/10.1007/s00436-008-0985-5

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  • DOI: https://doi.org/10.1007/s00436-008-0985-5

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