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Expressed sequence tag analysis of adult Clonorchis sinensis, the Chinese liver fluke

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Abstract

Expressed sequence tag (EST) pools represent partial profiles of the gene expressions of organisms. In an effort to construct a Clonorchis sinensis EST pool, 2,387 ESTs were collected from an adult C. sinensis cDNA library and assembled into 1,573 clusters. Of these clusters, 1,225 ESTs (51%) were singletons and 348 clusters consisted of more than two ESTs. There were 848 clusters (54%) that shared significant identity with previously reported proteins, and of these, 401 clusters were categorized into 11 major functional protein classes. Three cDNA clones of fructose-1,6-bisphosphate (FBP) aldolase were selected from the C. sinensis EST pool and analyzed for phylogenic clustering. FBP clones encoded a complete polypeptide, which shared significant identity to those of vertebrate and invertebrate animals and clustered with those of trematodes. We believe that the EST pool described can be confidently used as a platform in multigene researches on C. sinensis gene expression.

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Acknowledgement

This work was supported by the Korea Research Foundation Grant (KRF-2002-041-E20086).

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Authors and Affiliations

  1. Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul, 156-756, South Korea

    Pyo Yun Cho, Mi Jung Lee, Tae Im Kim, Shin-Yong Kang & Sung-Jong Hong

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  1. Pyo Yun Cho
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  2. Mi Jung Lee
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  3. Tae Im Kim
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  4. Shin-Yong Kang
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  5. Sung-Jong Hong
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Correspondence to Sung-Jong Hong.

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Cho, P.Y., Lee, M.J., Kim, T.I. et al. Expressed sequence tag analysis of adult Clonorchis sinensis, the Chinese liver fluke. Parasitol Res 99, 602–608 (2006). https://doi.org/10.1007/s00436-006-0204-1

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  • DOI: https://doi.org/10.1007/s00436-006-0204-1

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