Abstract
Chicken (Gallus gallus) were used as the experimental model for study of immune response against the microsporidium Encephalitozoon hellem (Didier et al., J Inf Dis 163:617–621, 1991) infection in birds. Two-day-old chicken were infected perorally or intraperitoneally with a dose of 107 spores of E. hellem. The anti-E. hellem immunoglobulin (Ig)A, IgY, and IgM antibody responses in sera and dropping sample extracts were determined by enzyme-linked immunosorbent assay. Results have shown specific antibody production in sera and intestinal secretions of infected birds. Chicken inoculated perorally developed the lowest antibody response. Microsporidian spores were not identified in the smears from cloacal swab samples of individual chicken. Intestinal segment cultures of perorally infected chicken cultivated in vitro showed the highest production of specific IgY and IgA antibodies in jejunum segments. In the further course of infection, the colon produced the highest amount of IgA, and the ileum and colon produced the highest amount of IgY.
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Acknowledgements
This study was supported by Ph.D. grant 524/03/H133 and a research project (Z60220518) of the Institute of Parasitology, Academy of Sciences of the Czech Republic. All experiments were done in accordance with the national law on the use of experimental animals, safety, and use of pathogenic agents.
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Saková, K., Sak, B., Ditrich, O. et al. Humoral response of chicken infected with the microsporidium Encephalitozoon hellem . Parasitol Res 98, 488–492 (2006). https://doi.org/10.1007/s00436-005-0089-4
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DOI: https://doi.org/10.1007/s00436-005-0089-4