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Local and remote cellular responses following a surgical lesion in the Cebus apella cerebral cortex

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Abstract

Distribution of bromodeoxyuridine immunoreactive (BrdU-IR) cell nuclei was analyzed at proximal and remote cortical sites in adult Cebus apella monkeys after a programmed surgical lesion placed either in the prefrontal or in the striate cerebral cortex. Increased GFAP-IR and vimentin-IR astrocytes, as well as IsolectinB4 labeled microglial cells, were observed both at lesional and perilesional areas. After injury at either location, the BrdU nuclear incorporation spread to supragranular layers in remote cortical areas functionally related to the injured cortex, probably due to involvement of degenerated cortico-cortical association fibers. Double labeling with Ki-67 suggested that remote BrdU-IR nuclei belong to proliferating cells, but the cell type remains to be determined, since these nuclei did not correspond to NeuN, MAP2, GFAP, Vimentin and isolectinB4 labeled cells. This remote effect should be incorporated to current clinical and experimental appraisal of cortical lesions.

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Abbreviations

IR:

Immunoreactivity/immunoreactive

BrdU:

Bromodeoxyuridine

Ki-67:

Nuclear antigen Ki67

lf:

Lateral fissure

ios:

Inferior occipital sulcus

iar:

Inferior arcuate sulcus

spcs:

Superior precentral sulcus

pos:

Parietooccipital sulcus

ips:

Intraparietal sulcus

cs:

Central sulcus

ps:

Principal sulcus

sts:

Superior temporal sulcus

mt:

Middle temporal sulcus

ifps:

Infraprincipal sulcus

cgs:

Cingulate sulcus

ros:

Rostral sulcus

ifg:

Inferior frontal gyrus

pcg:

Precentral gyrus

apg:

Anterior parietal gyrus

ag:

Angular gyrus

mfg:

Middle frontal gyrus

stg:

Superior temporal gyrus

mtg:

Middle temporal gyrus (adapted after Manocha et al. 1968)

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Acknowledgments

We are especially thankful to Dr. Rodolfo Tarelli and Dr. Fernando Pitossi for lending us the IsolectinB4, and to Prof. William Stallcup for the two NG2 antibody clones. Technical assistance by Dr. Carlos Nagle (Centro de Investigaciones en Reproducción Humana y Experimental, CIRHE) and Ms. Cristina Juárez (CONICET) is acknowledged, as well as administrative work by Mrs. Beatriz Stuto (CONICET). We also acknowledge the collaboration of Dr. Fernando Poletta (Centro Médico de Investigaciones Clínicas, CEMIC) in the statistical analysis of data. We are grateful to Prof. G. Paxinos for confirming the correct use of the actualized terminology for Cebus apella brain sulci and gyri. This work was supported in part by FONCYT (PICT# 01-03465; PICT# 14109); Fundación Conectar; CONICET (PIP# 740/98; PIP 5106/05); San Jorge Emprendimientos S.A.; Fundación Bunge y Born; Fundación René Baron and Corpomédica Argentina S.A.

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The authors declare that they have no conflict of interest.

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  1. Unidad de Neurobiología Aplicada (UNA), Centro Médico de Investigaciones Clínicas (CEMIC-CONICET), Av. Galván 4102, 1431, Buenos Aires, Argentina

    Ximena A. Lanosa, Alberto Yáñez, Sabina Alzugaray & Jorge A. Colombo

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  1. Ximena A. Lanosa
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  2. Alberto Yáñez
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  3. Sabina Alzugaray
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  4. Jorge A. Colombo
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Correspondence to Jorge A. Colombo.

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Lanosa, X.A., Yáñez, A., Alzugaray, S. et al. Local and remote cellular responses following a surgical lesion in the Cebus apella cerebral cortex. Brain Struct Funct 217, 485–501 (2012). https://doi.org/10.1007/s00429-011-0356-3

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