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Low prevalence of human papillomavirus infection in sinonasal inverted papilloma and oncocytic papilloma

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Abstract

The aim of this study was to investigate the role of human papillomavirus (HPV) in sinonasal inverted papilloma (SIP) and sinonasal oncocytic papilloma (SOP) from a single institution and whether p16 can serve as a surrogate marker for HPV infection. This study included 49 subjects with SIP and 36 subjects with SOP. Formalin-fixed paraffin-embedded tissues were used to extract genomic DNA, and HPV detection was performed by utilizing a valid nested polymerase chain reaction approach that can detect all known HPV subtypes. Immunohistochemistry was used to evaluate the expression of p16 in all tumor sections. The presence of HPV DNA was found in 6.1% (3/49) of the SIP patients and 11.1% (4/36) of the SOP patients. All identified HPV subtypes in SIP were high-risk HPV, including HPV-16 (two patients) and HPV-58 (one patient). Regarding SOP, there were three patients positive for HPV-16 and one with low-risk HPV (type 6). In total, 11/49 (22.4%) SIP lesions and 10/36 (27.8%) SOP lesions were considered p16 positive, with p16 staining in more than 70% of tumor cells. There was only one SIP and one SOP that were positive for both HPV (high-risk HPV type 16) and p16 staining. HPV does not play an etiologic role in inverted papilloma or oncocytic papilloma of the sinonasal region. p16 immunostaining should not be used as a surrogate marker to evaluate the HPV infection status in these lesions.

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Acknowledgments

This work was supported by Natural Science Foundation of China (No. 81371077) and Natural Science Foundation of Shanghai (16ZR1405000). The authors would like to thank Jun Wei and Qiang Wang from Shanghai ERA Biotech for their help for the technical issues.

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Authors and Affiliations

  1. Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, 83 Fen Yang Road, Shanghai, 200031, China

    Huan Wang, Juan Liu, Jingjing Wang, Xicai Sun, Li Hu & Dehui Wang

  2. Department of Clinical Pathology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China

    Changwen Zhai

  3. Department of Clinical Laboratory, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China

    Li Hu

  4. Department of Experimental Center, Eye, Ear, Nose and Throat Hospital, Fudan University, 83 Fen Yang Road, Shanghai, 200031, China

    Li Hu

Authors
  1. Huan Wang
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  2. Changwen Zhai
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  3. Juan Liu
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  4. Jingjing Wang
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  5. Xicai Sun
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  6. Li Hu
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  7. Dehui Wang
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Contributions

Huan Wang, Changwen Zhai, and Juan Liu performed the experiments and measurements, Huan Wang and Jingjing Wang processed the experimental data, performed the analysis, drafted the manuscript, and designed the figures. Dehui Wang aided in interpreting the results and worked on the manuscript. Xicai Sun and Li Hu were involved in planning and supervising the work and also the final approval of the manuscript. All authors discussed the results and commented on the manuscript.

Corresponding authors

Correspondence to Xicai Sun or Li Hu.

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Ethical approval

All procedures followed were in accordance with the ethical standards of the Research and Ethical Committee of EENT Hospital affiliated with Fudan University and with the Helsinki Declaration of 1975 (in its most recently amended version).

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Informed consent was obtained from all patients included in the study.

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The authors declare that they have no conflict of interest.

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This article is part of the Topical Collection on Quality in Pathology

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Wang, H., Zhai, C., Liu, J. et al. Low prevalence of human papillomavirus infection in sinonasal inverted papilloma and oncocytic papilloma. Virchows Arch 476, 577–583 (2020). https://doi.org/10.1007/s00428-019-02717-3

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  • DOI: https://doi.org/10.1007/s00428-019-02717-3

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