Abstract
Johnston’s organ (Jo) acts as an antennal wind-sensitive and/or auditory organ across a spectrum of insect species and its axons universally project to the brain. In the locust, this pathway is already present at mid-embryogenesis but the process of fasciculation involved in its construction has not been investigated. Terminal projections into the fine neuropilar organization of the brain also remain unresolved, information essential not only for understanding the neural circuitry mediating Jo-mediated behavior but also for providing comparative data offering insights into its evolution. In our study here, we employ neuron-specific, axon-specific, and epithelial domain labels to show that the pathway to the brain of the locust is built in a stepwise manner during early embryogenesis as processes from Jo cell clusters in the pedicel fasciculate first with one another, and then with the two tracts constituting the pioneer axon scaffold of the antenna. A comparison of fasciculation patterns confirms that projections from cell clusters of Jo stereotypically associate with only one axon tract according to their location in the pedicellar epithelium, consistent with a topographic plan. At the molecular level, all neuronal elements of the Jo pathway to the brain express the lipocalin Lazarillo, a cell surface epitope that regulates axogenesis in the primary axon scaffold itself, and putatively during fasciculation of the Jo projections to the brain. Central projections from Jo first contact the primary axon scaffold of the deutocerebral brain at mid-embryogenesis, and in the adult traverse mechanosensory/motor neuropils similar to those in Drosophila. These axons then terminate among protocerebral commissures containing premotor interneurons known to regulate flight behavior.
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Introduction
Johnston’s organ (Jo) functions as an antennal auditory organ across a spectrum of insect species (Tischner 1953; Manning 1967; Göpfert and Robert 2001a, b; Todi et al. 2004; Boekhoff-Falk 2005; Grob et al. 2021), mediates dance communication in bees (Brockmann and Robinson 2007), and as a wind-sensitive receptive system regulates flight behavior in locusts (Gewecke 1970, 1972) and Drosophila (Mamiya and Dickinson 2015; Patella and Wilson 2018). Afferents from Johnston’s organ have been shown to project from the antenna to the adult brain in several species including ant (Grob et al. 2021), Drosophila (Kamikouchi et al. 2006; Patella and Wilson 2018) and bee (Brockmann and Robinson 2007). In the bee, brain projections are organized topographically so as to conserve the spatial information encoded by the sensilla in the antenna (Ai et al. 2007).
To our knowledge, the only reports of projections from Jo into the brain of the adult locust are an observation based on Golgi histology (Williams 1972) and a cursory study using cobalt backfilling (see Fig. 1; Gewecke 1979). Both showed axons entering the deutocerebrum from the antennal nerve and terminating near the protocerebral midline but lacked information about the location of these central projections with respect to neuropilar regions (c.f. von Hadeln et al. 2018) and the commissural system (c.f. Boyan et al. 1993) of the brain. Such information would allow the circuitry mediating flight behavior to be resolved and provide comparative data offering insights into its evolution.
From a developmental perspective, neurogenesis in Johnston’s organ itself has been extensively studied in Drosophila (see Eberl and Boekhoff-Falk 2007; Jarman 2014; Singhania and Grueber 2014), mosquito (Schmidt 1967), moth (Keil and Steiner 1990; Keil 1997), cockroach (Blöchl and Selzer 1988), and locust (Boyan and Ehrhardt 2022), but axogenesis of the afferent pathway from Jo to the brain is less well understood. In holometabolous insects, its development commences during embryogenesis but only reaches maturity during the pupal to adult transition (see Singhania and Grueber 2014). In hemimetabolous insects, on the other hand, mechanosensory systems must be functional on hatching and we have shown that by mid-embryogenesis, the overall pattern of tracts associated with Jo in the locust antenna already strongly resembles that described for the adult (Boyan and Ehrhardt 2022). However, the process of axogenesis enabling afferents from Johnston’s organ to reach their targets in the brain has yet to be elucidated.
The locust antenna contains two major axon tracts and these are pioneered early in embryogenesis by neurons from its apical tip (Ho and Goodman 1982; Seidel and Bicker 2000; Boyan and Williams 2004; Ehrhardt et al. 2015). Axons from these pioneers project first to the deutocerebrum and then the protocerebrum where they target the primary axon scaffold of the brain (Boyan and Ehrhardt 2015). In this present study, we report that projections from cell clusters of Jo stereotypically associate with only one axon tract according to their location in the pedicellar epithelium, consistent with a topographic plan. At the molecular level, all neuronal elements of the Jo pathway to the brain express the lipocalin Lazarillo, a cell surface epitope that regulates axogenesis in the antennal axon scaffold (Ehrhardt et al. 2015). Central projections from Jo first contact the primary axon scaffold of the deutocerebral brain at mid-embryogenesis, and in the adult traverse mechanosensory/motor neuropils (von Hadeln et al. 2018) similar to those in bee, ant, and fly (Kamikouchi et al. 2006; Ai et al. 2007; Brockmann and Robinson 2007; Patella and Wilson 2018; Grob et al. 2021). In the locust, axon terminals from Jo are found among commissures containing the processes of premotor interneurons regulating flight behavior (Boyan et al. 1993).
Materials and methods
Animal source
Locusts (Schistocerca gregaria) used in this study derive from crowded colonies maintained at the Biocenter, Ludwig-Maximilians-Universität München (see Ehrhardt et al. 2015). Embryos were staged to the nearest 1% of developmental time (5% ≈ 24 h under colony conditions) according to Bentley et al. (1979). The results presented in this paper derive from experiments on over 80 embryonic and adult locusts.
Immunolabeling
Protocols for immunolabeling with primary and secondary antibodies, microscopy, and image processing were all as previously described (see Boyan and Williams 2004, 2007; Boyan and Niederleitner 2011; Ehrhardt et al. 2015).
The following antibodies were employed:
Primary antibodies
Anti-Horseradish peroxidase (α-HRP, polyclonal rabbit, Dianova) recognizes a neuron-specific cell surface epitope in insects (see Jan and Jan 1982).
Anti-Lazarillo (Mab 10E6, mouse, generous gift of D. Sánchez) recognizes the glycosylphosphatidylinositol (GPI)-linked cell surface lipocalin Lazarillo expressed by sensory and pioneer neurons in the locust embryo (see Sánchez et al. 1995; Ganfornina et al. 1995).
Anti-8B7 (Mab, mouse, generous gift of M. Bastiani) recognizes the Akt2 isoform of protein kinase B. In locusts, the Akt2 kinase is expressed early in development in neuroblasts and their progeny, later in axonal projections (see Seeger et al. 1993).
Anti-Lachesin (Mab 1C10, mouse, generous gift of M. Bastiani) recognizes the glycosylphosphatidylinositol (GPI)-linked cell surface molecule Lachesin belonging to the Ig superfamily (see Karlstrom et al. 1993). The expression occurs initially on all differentiating epithelial cells, but only cells involved in neurogenesis, such as precursors, continue to express the molecule later.
Secondary antibodies
Single labeling: against α -HRP involved Alexa® 488 (goat anti-rabbit, Invitrogen) or Cy3 (goat anti-rabbit, Dianova); against either α -Lazarillo, α -8B7, or α -Lachesin involved Cy3 (goat anti-mouse, Dianova).
Double labeling: against α -HRP/ α -Lazarillo involved Alexa® 488 (goat anti-rabbit, Invitrogen) for α -HRP and Cy3 (goat anti-mouse, Dianova) for α -Lazarillo; against α -HRP/ α -Lachesin involved Cy5 (donkey anti-goat, Dianova) for α -HRP/Alexa® 488 (donkey anti-mouse, Invitrogen) for α -Lachesin; against α -HRP/ α -8B7 involved Alexa® 488 (goat anti-rabbit, Invitrogen) for α -HRP/ Cy3 (goat anti-mouse, Dianova) for α -8B7.
Controls for the specificity of all secondary antibodies were (a) the lack of a staining pattern in the absence of the primary antibody and (b), in all cases, a staining pattern consistent with previously published data (see above).
Histology
Golgi histology
This employs silver impregnation to reveal neuronal morphology. The protocol for both adult and embryonic locusts basically followed the Golgi-Kopsch method of Colonnier as described in Williams (1972).
Bielschowsky histology
This employs silver impregnation to highlight mainly axonal membranes and/or neurofilaments. The protocol used is described in Boyan et al. (1993).
Embryonic preparations were sectioned at 14–16 µm thickness for microscopy, adult preparations at 25–100 µm thickness.
Nomenclature
This study applies body axes and the equivalent neuraxes (denoted by the affix "n") to brain anatomy. The nomenclature used to describe brain neuroarchitecture follows Boyan et al. (1993) for the commissural system, and Ito et al. (2014) and von Hadeln et al. (2018) for tracts and neuropils. The nomenclature of Gewecke (1979) is maintained for the projections of Jo afferents in the locust brain as these are not described elsewhere. Anatomical abbreviations are explained directly in the relevant figure legend.
Results
Background
Gewecke (1979) showed that axons from cell clusters of the Jo merge to form the Nervus pedicellaris lateralis (Npl) and then project into the deutocerebrum from the antennal nerve as the so-called Tractus Pedicello-Lobus Dorsalis (TPLD) (Fig. 1). The TPLD subsequently runs dorsally (n, anterior) and posteriorly (n, dorsal) to the antennal lobe and terminates as the Tractus Pedicello-Protocerebrum Posterior-medianus (TPPP) medially in the protocerebral brain. We confirm these pioneering findings but are unable to replicate Gewecke’s finding of a Tractus-Pedicello-ganglion Subesophagealis (TPSG) that splits from the TPLD itself and descends in the ventral nerve cord (VNC) to the subesophageal ganglion. The axons in this tract were revealed by cobalt backfilling from the VNC and purportedly derive from cells in the pedicel, but until their identity is clarified, we focus here on the protocerebral terminations in the TPPP tract which allow a direct comparison to be made with those in ant (Grob et al. 2021), Drosophila (Kamikouchi et al. 2006; Patella and Wilson 2018), and bee (Brockmann and Robinson 2007).
Axogenesis and fasciculation with pioneer antennal tracts
In hemimetabolous insects, mechanosensory structures such as Johnston’s organ (Jo) must be functional on hatching and so develop during embryogenesis (see Anderson 1973; Boyan and Ehrhardt 2022). We therefore began our investigation of axogenesis in the pathway from Jo to the brain at early embryonic stages. Immunolabeling of a neuron-specific cell surface epitope recognized by the antibody against horseradish peroxidase (α -HRP) shows that by 37% of embryogenesis (Fig. 2a), HRP-positive dorsal and ventral pioneer cells in the apical flagellum have generated two respective tracts that then merge at the antennal base and project within the antennal nerve towards the brain. The pathway from Jo itself to the brain has still to form as the cell clusters of Jo have yet to undergo axogenesis. However, double-immunolabeling with α -HRP and anti-Lachesin (α -Lach) (Fig. 2b) confirms that at 50% of embryogenesis, a Nervus pedicellaris lateralis (Npl; c.f. Fig. 1) comprising axons both from the Jo cell clusters in the pedicellar domain and the pioneer tracts is present, implying that construction of the afferent pathway from Jo to the brain must occur beforehand. Significantly, outgrowing axons from a Jo cell cluster are also clearly seen to fasciculate with a pioneer antennal tract (Fig. 2c) suggesting that these might act as a guidance system for Jo axons tracking to the brain.
A time series following immunolabeling with α -HRP (Fig. 2d) reveals that fasciculation between the axons from Jo cell clusters and the primary antennal tracts develops in a stepwise manner. At 40% of embryogenesis, the axonal growth cones of Jo cells generate initial filopodia but these are not yet specifically directed towards a pioneering antennal tract. At 41%, filopodia from cells in two clusters are now clearly directed towards an antennal tract that also sprouts multiple filopodia in this region. Filopodia from Jo cell clusters then extend basally in the antenna (42%), fasciculate with one another (43%), and also project onto a pioneer antennal tract (45%). At 50%, axons from several cell clusters can be seen projecting together onto a single pioneering tract, confirming our hypothesis.
Fasciculation is topographic
We next examined whether projections from Jo cell clusters fasciculate selectively with a given pioneer tract according to their location (dorsal/ventral) in the pedicellar domain. Evidence for this is provided by labeling with α -HRP which shows (Fig. 3a) dorsal and ventral cell clusters each clearly fasciculating with separate pioneer tracts. Repeat preparations at 45% (Fig. 3b, upper row) and 55% (Fig. 3b, lower row) of embryogenesis confirm this pattern of selective fasciculation. A series of 3D confocal images in which the antenna is rotated in a stepwise manner from dorsal to ventral at 55% of embryogenesis (Fig. 3c) demonstrates the topographic projection pattern of axons from the six dorsal and six ventral cell clusters in the pedicel to their respective pioneer antennal tracts.
Expression of a regulatory molecule during axogenesis
As elsewhere in the insect nervous system (see Dickson 2002), axogenesis of the Jo pathway to the brain is likely to involve a hierarchy of regulatory molecules among which are the GPI-linked cell surface lipocalins such as Lazarillo (see Ganfornina et al. 1995; Sánchez et al. 1995). We have previously shown (Suppl. Figure 1) that immunoblocking the Lazarillo epitope in whole embryo culture dysregulates axogenesis in the pioneer tracts of the locust antenna, consistent with findings in the central nervous system (Ganfornina et al. 1995; Sánchez et al. 1995).
Expression of the Lazarillo cell surface epitope in the Jo pathway to the brain was examined by double-immunolabeling with α -Lazarillo and α -HRP at progressive developmental stages (Fig. 4). At 39% of embryogenesis (Fig. 4a–c), pioneer neurons, the primary axon tracts, and cell clusters of Jo are HRP/Lazarillo-positive. The latter have yet to generate axon processes at this early stage (c.f. Fig. 2), but by 45% (Fig. 4d–f) cells of two ventral Jo clusters can be seen to extend co-labeled axon processes that fasciculate with one another and then target the co-labeled ventral tract. Subsequently (50%, Fig. 4g–i), the Lazarillo epitope is expressed in the complete pathway involving: (i) axon processes originating from Jo cell clusters, (ii) the primary ventral and dorsal tracts with which they fasciculate, (iii) the Nervus pedicellaris lateralis containing axons from both Jo and the primary antennal tracts, and (iv) the antennal nerve to the brain. We speculate that the ubiquitous expression we report here signals a wider regulatory role for Lazarillo in axogenesis of the complete pathway from Jo to the brain.
Projections from Johnston’s organ in the brain
Contact with the primary axon scaffold of the brain
Axons in the pioneer antennal tracts have been shown to enter the deutocerebrum of the brain from the antennal nerve at mid-embryogenesis before then targeting the primary axon scaffold of the protocerebrum (Boyan and Ehrhardt 2015). Given the selective fasciculation between axons from Jo and these pioneer tracts within the antenna itself (see Figs. 2 and 3), it seemed plausible that projections from Jo also follow this pre-established pathway into the central brain.
We first visualized the central projections from Jo along with the axon scaffold they target in the embryonic brain by immunolabeling with axon-specific α -8B7. A longitudinal section through the antennal base and right brain hemisphere at 50% of embryogenesis (Fig. 5a) shows 8B7-labeled axons from cell clusters of Jo merging to form the Nervus pedicellaris lateralis (Npl) and then entering the deutocerebrum from the antennal nerve as the Tractus Pedicello-Lobus Dorsalis (TPLD) (c.f. Fig. 1 for the adult brain). Axons in the TPLD can be seen to contact the primary axon scaffold of the brain in the mechanosensory region of the deutocerebrum. This lies posterior (n, dorsal) to the more glomerular region (Fig. 5b) containing the projections of olfactory antennal afferents (see Williams 1972; von Hadeln et al. 2018) as well as the axons of olfactory interneurons that project to the mushroom bodies via the medial antennal lobe tract (see Ito et al. 2014; von Hadeln et al. 2018).
A further embryonic (50%) preparation (Fig. 5c) reveals that after entering the deutocerebrum, the 8B7-positive Jo axons in the TPLD project further antero-medially (according to the neuraxis) in the TPPP tract and terminate in the protocerebrum as a dense aborization characterized by distinct blebs.
Axon tracts from Johnston’s organ and the neuroarchitecture of the brain
We then proceeded to examine the terminal projections from Johnston’s organ and potential brain neurons they might target, first using the well-established Golgi histological method (see Strausfeld 1976).
A longitudinal section of the right brain hemisphere at 85% of embryogenesis following Golgi histology (Fig. 6a) viewed from a posterior (n, dorsal) aspect shows the Tractus Pedicello-Lobus Dorsalis (TPLD) containing tightly bundled axon processes from the Jo in the deutocerebrum and projecting medially within the embryonic posterior slope (ePS), dorsal (n, anterior) to the developing lateral antennal mechanosensory and motor center (eLAMMC), and the antennal lobe. At the level of the embryonic medial antennal mechanosensory and motor center (eMAMMC) the axons then turn dorsally (n, anteriorly) and terminate as a dense arborization termed the Tractus Pedicello-Protocerebrum Posterior-medianus (TPPP) bordering the embryonic vest (not in view here). These protocerebral arborizations comprise two putative sub-fields and at this embryonic stage (85%) already bear a very similar overall morphology to those of the adult brain (Fig. 6b) where two sub-fields are again present. Tracts from other antennal afferents entering the deutocerebrum via the antennal nerve lie further ventral (n, posterior) to the TPLD and speculatively contribute to the ventral area of flagellar afferents (VFA).
Axon counts in five preparations following Golgi histology reveal the TPPP to comprise 12, 12, 12, 10, 11 stained axons varying in diameter from 2 to 4 µm. At higher resolution (Fig. 6c), the prominent terminal blebs visible after 8B7 immunolabeling (c.f. Figure 5c) are again evident. We propose that these terminals glance the MAMMC, lie within the PS, and border the more medial VES. In favorable preparations (Fig. 6d), the Golgi method reveals axon projections from Jo terminating in two sub-fields of the TPPP and extending towards the commissural system (nDC XV (= GC), nDC XVI, nDC XVII) of the central brain. Also, stained are the protocerebral processes of identified premotor interneurons such as the bilateral descending contralateral movement detector (DCMD) neurons whose axons cross the brain midline in nDC XVII (c.f. Fig. 7 below) and then project to thoracic motor centers.
To definitively place the axon projections from Jo within the neuroarchitecture of the adult locust brain, we employed the map of brain neuropils provided by Ito et al. (2014) and von Hadeln et al. (2018). Our schematic reconstruction (Fig. 7a) shows Jo axons projecting as the TPLD into the deutocerebrum via the antennal nerve, running medially through the dorsal (n, anterior) regions of LAMMC and MAMMC and terminating as the TPPP in the posterior slope (PS) of the protocerebrum.
Sectioning following Bielschowsky histology allows the Jo axons together with the surrounding neuropilar regions and commissural tracts of the adult brain using published maps (Boyan et al. 1993; Ito et al. 2014; von Hadeln et al. 2018) to be visualized (Fig. 7b). Bundled axons from Jo of each antenna project symmetrically into the neuropil of each deutocerebral hemisphere via the TPLD tract. These axons traverse the LAMMC and MAMMC regions and run to the posterior slope (PS) near nDC XVI and nDC XVII of the protocerebrum. Repeat preparations following Bielschowsky histology consistently reveal 12 axons in this tract.
In order to analyze the central projections from the adult Jo at the single cell level, we reverted to Golgi histology, generated computer-based autotracings from two individual stained axons, and superimposed these onto the TPLD and TPPP tracts within our commissural map (Fig. 7c). Both stained Jo axons can be seen to closely follow the overall projection through LAMMC and MAMMC towards the PS (c.f. Fig. 7a). The axons terminate as two sub-fields, the most dorsal (n, anterior) field lies adjacent to commissures nDC XVI and nDC XVII containing the axons of identified multimodal premotor interneurons DNC, DCMDcc (Fig. 7d) and DCMD (c.f. Fig. 6d).
Discussion
Axogenesis of the pathway to the brain
Our study shows that in keeping with its hemimetabolous lifestyle, the afferent pathway from Johnston’s organ (Jo) to the brain of the locust Schistocerca gregaria is constructed during embryogenesis and that the primary axon scaffold of the antenna pioneered earlier by the dorsal and ventral apical pioneers (Fig. 2a) plays a key role in the process. Axogenesis commences in Jo at around 40% of embryogenesis and proceeds in a stepwise manner as the growth cones from neurons of any given cell cluster fasciculate first with one another, and then with one of the pioneer tracts (Fig. 2d). Fasciculation is selective in that cell clusters located dorsally in the pedicellar epithelial domain target the dorsal pioneer tract, and ventrally located clusters the ventral pioneer tract (Fig. 3). This topographic pattern ensures that the spatial information encoded by the sensilla from these respective cell clusters is conserved in their projections at least to the antennal base.
At the molecular level, all neuronal elements of the afferent pathway from Jo to the brain express Lazarillo (Fig. 4), one of the GPI-linked cell surface lipocalins known to regulate pioneer growth cone guidance in the locust nervous system (Chang et al. 1992; Ganfornina et al. 1995; Sánchez et al. 1995; Goodman 1996). We have shown that antibody blocking of the Lazarillo epitope alone is sufficient to dysregulate fasciculation of pioneer axons in the early antenna itself (Suppl. Figure 1; Ehrhardt et al. 2015) and speculate that Lazarillo also plays a role in regulating the fasciculation of more downstream elements in the pathway from Jo to the brain.
Central projections
The embryonic brain
Although the axons from cell clusters of Johnston’s organ project selectively to either the ventral or dorsal pioneer tracts (Fig. 3), these merge at the antennal base to form the Nervus pedicellaris lateralis (Figs. 1, 2a, b, 4g–i) and so represent a single projection into the antennal nerve. In order to then preserve the topography encoded peripherally, the axons from Jo would have to maintain stereotypic locations in the antennal nerve and subsequent brain tracts. This topographic morphology is present for the axons from premotor brain neurons descending in the ventral nerve cord (Fig. 7c; c.f. Williams 1975) but has not yet been demonstrated for the afferent pathway from Jo to the locust brain. Selective labeling of dorsal and ventral clusters via combined immunolabeling and intracellular dye injection as previously used to identify pioneers and nerve tract associated cells in the embryonic antennal nervous system (Boyan and Williams 2007) could clarify the issue (c.f. Ai et al. 2007 for bee).
The projection from Jo reaches the deutocerebrum of the brain prior to mid-embryogenesis (Fig. 5a) and follows the route established earlier by the antennal pioneers (Boyan and Ehrhardt 2015). Axons target the embryonic striate, mechanosensory neuropils of the deutocerebrum (Fig. 5a) and so bypass its glomerular region (Fig. 5b) where the afferents from odor-detecting sensilla terminate (Hansson et al. 1996; Strausfeld 2009), confirming previous reports for the adult (Williams 1972; Gewecke 1979). How these embryonic mechanosensory neuropils translate into the mature AMMC with its LAMMC and MAMMC subdivisions (von Hadeln et al. 2018) remains to be established.
Axon-specific immunolabeling (Fig. 5c) reveals that the embryonic Jo axons progress medially through the brain in the so-called Tractus Pedicello-Lobus Dorsalis (TPLD; c.f. Figure 1) and contact the primary axon scaffold of the protocerebrum where they terminate as the Tractus Pedicello-Protocerebrum Posterior-medianus (TPPP) at mid-embryogenesis. At 85% of embryogenesis (Fig. 6a), these projections may be putatively allocated to the posterior slope (PS) and their terminals are morphologically very similar to those reported for the adult (Figs. 1, 6b–d).
Conserved tracts in the adult brain
Gewecke (1979) reports the TPPP of the adult locust to be a dense bundle comprising approximately 12 axons, and our axon counts based on Golgi (Fig. 6) and Bielschowsky (Fig. 7b) histology match his findings, implying that each of the 12 cell clusters contributes a single axon to the TPPP. Inspection of our 3D confocal data here (Fig. 3c) and previously (Boyan and Ehrhardt 2022) suggests this is the case. Despite the axons in TPLD and TPPP remaining bundled, their terminations appear to form two putative sub-fields (1, 2; Fig. 6c), but it remains to be proven whether these reflect the dorsal/ventral location of cell clusters in the pedicellar epithelium and therefore a topographic projection in the brain as demonstrated for the bee (Ai et al. 2007) and ant (Grob et al. 2021) where three terminal zones (T6I, T6III-I, T6III-II) reflect the posterior, anterior, and ventral scolopidial fields of Jo.
Gewecke (1979) hypothesized that the TPPP of the locust brain might be homologous to a tract in the bee referred to as B41 by Pareto (1972), and as T6I by Suzuki (1975). More recent findings on the bee (Ai et al. 2007) and desert ant Cataglyphis (Grob et al. 2021) each show subsets of fibers from Jo projecting to the protocerebrum via a branch these authors also refer to as T6I and which bear a great similarity to subfield 1 of the TPPP in the locust (c.f. Figs. 5c, 6b).
Our data show that Jo axons are found in subregions of the antennal mechanosensory and motor center (AMMC) such as LAMMC and MAMMC and also appear to target the posterior slope (PS). Pedicellar and scapal mechanoreceptors have been shown to target the MAMMC of the locust (von Hadeln et al. 2018) consistent with data from bee, ant, and fly (Kamikouchi et al. 2006; Brockmann and Robinson 2007; Patella and Wilson 2018). These parallels suggest that information from Johnston’s organ is represented in evolutionarily conserved neuropils in the insect brain.
Brain neuroarchitecture and behavior
Resolving the neuronal circuitry mediating Johnston’s organ-based behavior requires knowledge about the location of Jo afferents with respect to the fine neuroarchitecture of the adult locust brain. Our present study utilizes a detailed map of brain neuropils (von Hadeln et al. 2018) and a groundplan of commissures (Boyan et al. 1993) to map the terminals of these afferents to a restricted subset of mechanosensory neuropils (AMMC, PS) and commissures (nDC XVI, nDC XVII) in the brain. Molecular and cellular evidence (Boyan et al. 2003; Ito et al. 2014) confirms that these commissures are protocerebral, as deutocerebral cells choose a protocerebral commissure (nPC XX) dorsal (n, anterior) to the esophagous or a tritocerebral (TCC, nPC XXIV) ventral (n, posterior) to the esophagous to cross the midline.
Commissures nDC XVI and nDC XVII are known to contain the processes of identified multimodal interneurons (Fig. 7; O'Shea and Williams 1974; O'Shea et al. 1974) whose axons descend in the ventral nerve cord to thoracic motor regions where they regulate course corrective flight behavior (Rowell 1971, 1988; Bacon and Möhl 1979; Griss and Rowell 1986). Morphological overlap and synaptic connections between Jo afferents and such premotor interneurons have yet to be demonstrated in the locust, but outputs from wind-sensitive antennal afferents to flight motor systems via descending interneurons have been reported in Drosophila (Mamiya and Dickinson 2015). With both the terminals of the Jo afferents now positioned with respect to identified brain neuropils, and an identified subset of commissures containing the axons of known premotor interneurons, the locust brain offers an excellent opportunity to unravel the circuitry involved in Jo-mediated behavior. Future comparisons with the data from Drosophila (Mamiya and Dickinson 2015) can offer insights into evolutionary aspects of this behavior.
Data availability
Core data supporting this study are available on request from Dr. E.E. Ehrhardt, AG Ito, Institute of Zoology, Universität Köln, Zülpicher Str 47b, 50,674 Cologne, Germany.
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Acknowledgements
We thank Dr. Tatjana Kleele for assistance with immunolabeling and confocal microscopy, and Karin Fischer for excellent technical assistance.
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Open Access funding enabled and organized by Projekt DEAL. This study was funded by the Graduate School of Systemic Neuroscience, Biocenter, Ludwig-Maximilians-Universität München.
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G.B., L.W., and E.E. wrote and checked the text of the manuscript. G.B., L.W., and E.E. conceived, created, and checked all the figures. All three authors agree on the content and format of the final manuscript.
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All experiments strictly followed the guidelines for animal welfare as laid down by the Deutsche Forschungsgemeinschaft (DFG). Accordingly, larval and adult locusts were routinely anesthetized by either cold or CO2 prior to experimentation.
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Boyan, G., Williams, L. & Ehrhardt, E. Central projections from Johnston’s organ in the locust: Axogenesis and brain neuroarchitecture. Dev Genes Evol 233, 147–159 (2023). https://doi.org/10.1007/s00427-023-00710-0
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DOI: https://doi.org/10.1007/s00427-023-00710-0