Abstract
Three y-type high-molecular-weight (HMW) glutenin gene open reading frames (ORFs), Chiy1, Chiy2, and Racy, were isolated and characterized from Leymus chinensis PI499516 and Leymus racemosus ssp. racemosus W623305. They shared an extra glutamine in the N-terminal and LAAQLPAMCRL peptides in the C-terminal with x-type HMW glutenins but had different N-terminal lengths. Like other y-type HMW glutenins, Chiy2 and Racy had 104 (or 105) amino acid (aa) residues at the N-terminal and started with EGEASR, whereas Chiy1 had 99 aa in this domain and started with QLQCER because of the deletion of EGEASR. Five other y-type glutenins, including those from Elymus ciliaris, Pseudoroegneria libanotica, and Leymus mollis, were similar to Chiy1. The ORF of Chiy2 was probably not expressed. The ORFs of both Chiy1 and Racy were expressed in bacteria. The maximum likelihood phylogenic tree based on the signal peptide and N-terminal and C-terminal aa residues revealed two clades of y-type HMW glutenins in Triticeae; the first contained Ay, By, Cy, Dy, Eey, Gy, Ky, Ry, Tay, and Uy, while the second clade contained the remaining y types, including those from Leymus. Within the second clade, HMW glutenins lacking the EGEASR peptide formed a subclade. These y-type HMW glutenins in Leymus could not be targeted to the Xm or Ns genome.
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References
Dewey DR (1984) The genome system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson JP (ed) Gene manipulation in plant improvement. Plenum, New York, pp 209–279
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Guo ZF, Yan ZH, Wang JR, Wei YM, Zheng YL (2005) Characterization of HMW prolamines and their coding sequences from Crithopsis delileana. Hereditas 142:56–64
Jiang QT, Wei YM, Lu ZX, Pu ZE, Lan XJ, Zheng YL (2010) Structural variation and evolutionary relationship of novel HMW glutenin subunits from Elymus glaucus. Hereditas 147:136–141
Liu SW, Zhao F, Gao X, Chen FG, Xia GM (2010) A novel high molecular weight glutenin subunit from Australopyrum retrofractum. Amino Acids 39:385–392
Liu ST, Zhu XL, Liu SW, Tan Y (2012a) Isolation and characterization of Glu-1 genes from the St genome of Pseudoroegneria libanotica. Gene 499:154–159
Liu J, Zhang B, Liu BL, Li HQ, Wang Z, Liu Q, Wei L, Liu DC, Zhang HG (2012b) Molecular cloning and analysis of a novel HMW-GS gene Glu1-St1.5 from Elymus sibiricus in Qinghai-Tibetan Plateau. Sci Res Essays 7:2189–2194
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120
Pistón F, Shewry PR, Barro F (2007) D hordeins of Hordeum chilense: a novel source of variation for improvement of wheat. Theor Appl Genet 115:77–86
Shewry PR, Halford NG, Tatham AS, Popineau Y, Lafiandra D, Belton PS (2003) The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nutr Res 45:219–302
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Thompson JD, Higgins DG, Gibson TJ (1994) Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Wang RR, Bothmer RV, Dvořák J, Fedak G, Linde-Laursen I, Muramatsu M (1994) Genome symbols in the Triticeae (Poaceae), In: Wang RR, Jensen KB, Jaussi C (eds) Proceedings of the 2nd International Triticeae Symposium, Logan, Utah, USA, pp 29–34
Wang JR, Yan ZH, Wei YM, Zheng YL (2006) Characterization of high molecular weight glutenin subunit genes from Elytrigia elongata. Plant Breed 125:89–95
Wang XQ, Yan ZH, Dai SF, Liu DC, Wei YM, Zheng YL (2012) Molecular characterization of four HMW glutenin genes from Heteranthelium piliferum C. E. Hubbard and Henrardia persica (Banks et Solander) Hochstetter. Genet Resour Crop Evol 59:1309–1318
Yan ZH, Wan YF, Liu KF, Zheng YL, Wang DW (2002) Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chin Sci Bull 47:220–225
Yan ZH, Wei YM, Wang JR, Liu DC, Dai SF, Zheng YL (2006) Characterization of two HMW glutenin subunit genes from Taenitherum Nevski. Genetica 127:267–276
Yang F, Yan ZH, Dai SF, Han C, Hu XG (2010) Isolation and characterization of upstream sequences of two high molecular weight glutenin subunit genes from Psathyrostachys juncea. J Sichuan Agric Univ 28:397–402
Yuan ZW, Chen QJ, Zhang LQ, Yan ZH, Zheng YL, Liu DC (2009) Molecular characterization of two silenced y-type genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum. J Integr Plant Biol 51:93–99
Zhang HB, Dvořák J (1991) The genome origin of tetraploid species of Leymus (Poaceae: Triticeae) inferred from variation in repeated nucleotide sequences. Am J Bot 78:871–884
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The authors acknowledge the financial support by the National Key Technologies R&D Program (2011BAD35B03) and the National Natural Science Foundation of China (30671272 and 31230053).
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Communicated by Sureshkumar Balasubramanian
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Sun, Y., Pu, Z., Dai, S. et al. Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus . Dev Genes Evol 224, 57–64 (2014). https://doi.org/10.1007/s00427-013-0455-7
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DOI: https://doi.org/10.1007/s00427-013-0455-7