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A novel high molecular weight glutenin subunit from Australopyrum retrofractum

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Abstract

We describe the sequence of a gene encoding a high molecular weight glutenin subunit (HMW-GS) expressed in the endosperm of the wheat relative Australopyrum retrofractum. Although the subunit has a similar primary structure to that HMW-GS genes present in other Triticeae species, its N-terminal domain is shorter, its central repetitive domain includes a unique dodecameric motif, and its C-terminal domain contain an extra cysteine residue. A phylogenetic analysis showed that the Glu-W1 gene is neither a true x- nor a true y-type subunit, although it is more closely related to the y-type genes present in the K and E genomes than to any other published HMW-GS gene. All these results indicated that this novel subunit may undergo a special evolutionary process different from other Triticeae species. A flour supplementation experiment showed that the Glu-W1 subunit has a negative effect on dough quality, which might be the result of interaction between the two closely placed cysteine residues in the C-terminal region.

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Acknowledgments

This work was supported by National Natural Science Foundation of China No. 30871320, National 863 High Technology Research and Development Project 2006AA100102 and National Transgenic Project 2009ZX08002-014B.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, 250100, Jinan, People’s Republic of China

    Shuwei Liu, Feng Zhao, Xin Gao, Fanguo Chen & Guangmin Xia

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  1. Shuwei Liu
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  2. Feng Zhao
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  3. Xin Gao
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  4. Fanguo Chen
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  5. Guangmin Xia
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Correspondence to Guangmin Xia.

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Liu, S., Zhao, F., Gao, X. et al. A novel high molecular weight glutenin subunit from Australopyrum retrofractum . Amino Acids 39, 385–392 (2010). https://doi.org/10.1007/s00726-009-0450-5

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