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SbYS1 and SbWRKY72 regulate Cd tolerance and accumulation in sweet sorghum

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Abstract

Main conclusion

SbYS1 and its upstream transcription factor SbWRKY72 were involved in Cd tolerance and accumulation and are valuable for developing sweet sorghum germplasm with high-Cd tolerance or accumulation ability through genetic manipulation.

Abstract

Cadmium (Cd) is highly toxic and can severely affect human health. Sweet sorghum, as an energy crop, shows great potential in extracting cadmium from Cd-contaminated soils. However, its molecular mechanisms of Cd-tolerance and -accumulation remain largely unknown. Here, we isolated a YSL family gene SbYS1 from the sweet sorghum genotype with high Cd accumulation ability and the expression of SbYS1 in roots was induced by cadmium. GUS staining experiment exhibited that SbYS1 was expressed in the epidermis and parenchyma tissues of roots. Further subcellular localization analysis suggested that SbYS1 was localized in the endoplasmic reticulum and plasma membrane. Yeast transformed with SbYS1 exhibited a sensitive phenotype compared to the control when exposed to Cd-NA (chelates of cadmium and nicotianamine), indicating that SbYS1 may absorb cadmium in the form of Cd-NA. Arabidopsis overexpressing SbYS1 had a longer root length and accumulated less Cd in roots and shoots. SbWRKY72 bound to the promoter of SbYS1 and negatively regulated the expression of SbYS1. Transgenic Arabidopsis of SbWRKY72 showed higher sensitivity to cadmium and increased cadmium accumulation in roots. Our results provide references for improving the phytoremediation efficiency of sweet sorghum by genetic manipulation in the future.

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Abbreviations

DMA:

Deoxymugineic acid

IRT:

Iron-regulated transporter

NA:

Nicotianamine

YSL:

Yellow Stripe-Like

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Acknowledgements

This work was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences (Precision Seed Design and Breeding, XDA24010306) and the National Natural Science Foundation of China (31971837).

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  1. Weitao Jia and Zijing Guo contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, Beijing, 100093, China

    Weitao Jia, Zijing Guo, Sulian Lv, Kangqi Lin & Yinxin Li

  2. China National Botanical Garden, Beijing, China

    Zijing Guo, Sulian Lv, Kangqi Lin & Yinxin Li

  3. Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 401122, China

    Weitao Jia

  4. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China

    Zijing Guo & Kangqi Lin

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  1. Weitao Jia
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LY: conceived and supervised this research, and revised the manuscript. JW: wrote the original draft, JW and GZ: performed experiments and analyzed data. LK and LS: helped revise and check the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yinxin Li.

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Jia, W., Guo, Z., Lv, S. et al. SbYS1 and SbWRKY72 regulate Cd tolerance and accumulation in sweet sorghum. Planta 259, 100 (2024). https://doi.org/10.1007/s00425-024-04388-0

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