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N6-Methyladenosine dynamic changes and differential methylation in wheat grain development

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Abstract

Main conclusion

More methylation changes occur in late interval than in early interval of wheat seed development with protein and the starch synthesis-related pathway enriched in the later stages.

Abstract

Wheat seed development is a critical process to determining wheat yield and quality, which is controlled by genetics, epigenetics and environments. The N6-methyladenosine (m6A) modification is a reversible and dynamic process and plays regulatory role in plant development and stress responses. To better understand the role of m6A in wheat grain development, we characterized the m6A modification at 10 day post-anthesis (DPA), 20 DPA and 30 DPA in wheat grain development. m6A-seq identified 30,615, 30,326, 27,676 high confidence m6A peaks from the 10DPA, 20DPA, and 30DPA, respectively, and enriched at 3'UTR. There were 29,964, 29,542 and 26,834 unique peaks identified in AN0942_10d, AN0942_20d and AN0942_30d. One hundred and forty-two genes were methylated by m6A throughout seed development, 940 genes methylated in early grain development (AN0942_20d vs AN0942_10d), 1542 genes in late grain development (AN0942_30d vs AN0942_20d), and 1190 genes between early and late development stage (AN0942_30d vs AN0942_10d). KEGG enrichment analysis found that protein-related pathways and the starch synthesis-related pathway were significantly enriched in the later stages of seed development. Our results provide novel knowledge on m6A dynamic changes and its roles in wheat grain development.

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Data availability statement

The supplementary data can be found online BioProject: PRJNA770512 (https://dataview.ncbi.nlm.nih.gov/object/PRJNA770512?reviewer=2h3bbvs90976rk4vj0q1mfmkk1).

Abbreviations

DPA:

Day post-anthesis

m6A:

N6-Methyladenosine

UTRs:

Untranslated regions

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Funding

This study was financially supported by a Research Foundation for Talented Scholars from Anhui Agricultural University and the introduced leading talent research team for Universities in Anhui Province, and a graduate Innovation Fund of Anhui Agricultural University (2021yjs-1).

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Authors and Affiliations

  1. College of Agronomy, Anhui Agricultural University, Hefei, 230036, Anhui, China

    Wenxiang Li, Yi Yu, Xuanrong Chen, Qian Fang, Anqi Yang, Xinyu Chen, Lei Wu, Chengyu Wang, Dechuan Wu & Dexiang Wu

  2. Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow and Huai River Valley, Ministry of Agriculture, Hefei, 230036, China

    Chengyu Wang

  3. Cotton Institute, Anhui Academy of Agricultural Sciences, Hefei, 230001, Anhui, China

    Sihong Ye

  4. Biology Department, Saint Mary’s University, Halifax, NS, B3H 3C3, Canada

    Genlou Sun

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  1. Wenxiang Li
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Correspondence to Sihong Ye, Dexiang Wu or Genlou Sun.

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Li, W., Yu, Y., Chen, X. et al. N6-Methyladenosine dynamic changes and differential methylation in wheat grain development. Planta 255, 125 (2022). https://doi.org/10.1007/s00425-022-03893-4

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