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A MADS-box transcription factor from grapevine, VvMADS45, influences seed development

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Abstract

Grape is one of the most economically important fruits worldwide, and seedless fruit is a main target in grape breeding. The MADS-box genes encode a large family of transcription factors in higher plants, and serve various developmental roles including flower, fruit and seed development. However, little is known about function of MADS-box genes in grapevine, especially related to seed development. Here, we characterized a role for the grapevine MADS-box gene, VvMADS45, in seed development. Heterologous expression of VvMADS45 (VvMADS45-OE) in tomato resulted in larger flowers, fruit and seeds. In contrast, disruption of a tomato homolog of VvMADS45 (SlAGL104-KO) resulted in smaller flowers, fruit and seeds. In addition, both VvMADS45-OE and SlAGL104-KO lines produced fewer seeds than control. The fraction of pollen grains that appeared abnormal or failed to rehydrate was greater in VvMADS45-OE lines than in non-transgenic control plants, while relatively few abnormal pollen grains were formed in SlAGL104-KO lines. Expression of several genes related to flower, fruit and seed development was altered in both VvMADS45-OE and SlAGL104-KO lines. These results revealed that VvMADS45 participates in flower, fruit and seed development, especially seed development, which may provide new insight into the genetic basis of seedlessness in grapes.

Key Message

Heterologous expression of grapevine VvMADS45 in tomato increases the size of flowers, fruit and seeds, and decreases seed number, while disruption of the tomato homolog of this gene, SlAGL104, decreases the size of the flowers, fruit, and seeds, as well as seed number.

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Acknowledgements

We thank Prof. Yaoguang Liu of South China Agricultural University for providing the pYLCRISPR/Cas9 system. This work was supported by the National Natural Science Foundation of China (U1603234), as well as the Program for Innovative Research Team of Grape Germplasm Resources and Breeding (2013KCT-25).

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  1. State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Xiaomeng Sun, Songlin Zhang, Xingmei Li, Xiuming Zhang, Xianhang Wang, Zhi Li & Xiping Wang

  2. Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Xiaomeng Sun, Songlin Zhang, Xingmei Li, Xiuming Zhang, Xianhang Wang, Zhi Li & Xiping Wang

  3. College of Horticulture, Agricultural University of Hebei, Baoding, 071001, Hebei, China

    Li Wang

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XPW and XMS designed experiments. XMS performed experiments. SLZ, XML, XHW, XMZ and ZL assisted with experiments. XMS and XPW wrote the manuscript. All authors approved the final manuscript.

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Sun, X., Zhang, S., Li, X. et al. A MADS-box transcription factor from grapevine, VvMADS45, influences seed development. Plant Cell Tiss Organ Cult 141, 105–118 (2020). https://doi.org/10.1007/s11240-020-01771-z

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