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Different dehydrins perform separate functions in Physcomitrella patens

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Abstract

Main conclusion

Dehydrins, PpDHNA and PpDHNB from Physcomitrella patens provide drought and cold tolerance while PpDHNC shows antimicrobial property suggesting different dehydrins perform separate functions in P. patens.

The moss Physcomitrella patens can withstand extremes of environmental condition including abiotic stress such as dehydration, salinity, low temperature and biotic stress such as pathogen attack. Osmotic stress is inflicted under both cold and drought stress conditions where dehydrins have been found to play a significant protective role. In this study, a comparative analysis was drawn for the three dehydrins PpDHNA, PpDHNB and PpDHNC from P. patens. Our data shows that PpDHNA and PpDHNB play a major role in cellular protection during osmotic stress. PpDHNB showed several fold upregulation of the gene when P. patens was subjected to cold and osmotic stress in combination. PpDHNA and PpDHNB provide protection to enzyme lactate dehydrogenase under osmotic as well as freezing conditions. PpDHNC possesses antibacterial activity and thus may have a role in biotic stress response. Overexpression of PpDHNA, PpDHNB and PpDHNC in transgenic tobacco showed a better performance for PpDHNB with respect to cold and osmotic stress. These results suggest that specific dehydrins contribute to tolerance of mosses under different stress conditions.

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Abbreviations

LEA:

Late embryogenesis abundant

LB:

Luria–Bertani media

LDH:

Lactate dehydrogenase

CaMV 35S:

Cauliflower Mosaic Virus (CaMV) 35S promoter

MDA:

Malondialdehyde

PCR:

Polymerase chain reaction

MIC:

Minimum inhibitory concentration

BSA:

Bovine serum albumin

GST:

Glutathione S-transferase

HRP:

Horse radish peroxidase

PBS:

Phosphate buffer saline

BAP:

6-Benzyl aminopurine

NAA:

1-Napthalene acetic acid

ROS:

Reactive oxygen species

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Acknowledgments

This work is supported by grants to S.R. from the Department of Science and Technology, Government of India. T.A. and G.U. thank the Department of Science and Technology, Government of India for Research Fellowships. T.H. thank the Council of Scientific and Industrial Research, Government of India for Research Fellowship.

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Authors and Affiliations

  1. Department of Botany, Centre of Advanced Study, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, India

    Tanushree Agarwal, Gouranga Upadhyaya, Tanmoy Halder & Sudipta Ray

  2. Division of Plant Biology, Bose Institute, P1/12 CIT Scheme VII M, Kolkata, 700054, India

    Abhishek Mukherjee & Arun Lahiri Majumder

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  1. Tanushree Agarwal
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  2. Gouranga Upadhyaya
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  3. Tanmoy Halder
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Correspondence to Sudipta Ray.

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Agarwal, T., Upadhyaya, G., Halder, T. et al. Different dehydrins perform separate functions in Physcomitrella patens . Planta 245, 101–118 (2017). https://doi.org/10.1007/s00425-016-2596-1

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