Different abiotic stress conditions induce distinct sets of anthocyanins, indicating that anthocyanins have different biological functions, or that decoration patterns of each anthocyanin are used for unique purposes during stress.
The induction of anthocyanin accumulation in vegetative tissues is often considered to be a response of plants to biotic or abiotic stress conditions. Arabidopsis thaliana (Arabidopsis) accumulates over 20 anthocyanins derived from the anthocyanidin cyanidin in an organ-specific manner during development, but the anthocyanin chemical diversity for their alleged stress protective functions remains unclear. We show here that, when grown in various abiotic stress conditions, Arabidopsis not only often accumulates significantly higher levels of total anthocyanins, but different stress conditions also favor the accumulation of different sets of anthocyanins. For example, the anthocyanin patterns of seedlings grown at pH 3.3 or in media lacking phosphate are very similar and characterized by relatively high levels of the anthocyanins A8 and A11. In contrast, anthocyanin inductive conditions (AIC) provided by high sucrose media are characterized by high accumulation of A9* and A5 relative to other stress conditions. The modifications present in each condition correlate reasonably well with the induction of the respective anthocyanin modification enzymes. Taken together, our results suggest that Arabidopsis anthocyanin profiles provide ‘fingerprints’ that reflect the stress status of the plants.
Anthocyanins are flavonoid pigments responsible for many of the red, violet and purple colors characteristic of fruits and flowers, where they function as attractants for pollinators or seed-dispersing organisms (Grotewold 2006). In many plant species, anthocyanins accumulate transiently in the epidermal cell layer of vegetative tissues at specific stages of development, such as leaf expansion (Parkin 1903), likely playing a role in photoprotection (Hatier and Gould 2009). However, abiotic stresses can induce anthocyanin synthesis in the chlorenchyma cells of the leaves of most plant species (Parkin 1903). The function of stress-induced anthocyanins is presently not known; one prominent hypothesis is that they serve as antioxidants that quench ROS (reviewed by Gould 2004a; Hatier and Gould 2009; Agati et al. 2012). ROS are mainly produced in chloroplasts and mitochondria via the aerobic reactions of photosynthesis and respiration, and accumulate to relatively high levels under stress conditions that limit photosynthesis (Mittler 2002; Rhoads et al. 2006). Anthocyanins are mainly sequestered in vacuoles, however, the enzymes of flavonoid biosynthesis are believed to be localized mainly on the cytosolic face of the ER, anchored to the membrane by cytochrome P450s such as flavonoid 3′-hydroxylase (F3′H) (Winkel 2004). Despite the different subcellular localizations of anthocyanins and ROS, anthocyanin-containing leaf cells have been shown to exhibit greater capacity to remove H2O2 than cells that lack these compounds (Gould et al. 2002).
Abiotic stresses that induce anthocyanin synthesis include drought in rice and Arabidopsis (Basu et al. 2010; Sperdouli and Moustakas 2012), cold in maize, Arabidopsis, and citrus (Christie et al. 1994; Crifò et al. 2011), high salt in tomato and red cabbage (Eryılmaz 2006), nutrient deficiency in Arabidopsis, hibiscus, and carrot (Mizukami et al. 1991; Rajendran et al. 1992; Jiang et al. 2007), osmotic stress in carrot callus and grapevine cell cultures (Rajendran et al. 1992; Suzuki 1995), and exposure to low pH of the medium in strawberry suspension cell cultures (Zhang and Furusaki 1997; reviewed by Chalker-Scott 1999; Winkel-Shirley 2002). The presence of sucrose in the culture medium also induces anthocyanin synthesis by a mechanism dependent on the MYB transcription factor, PAP1 (Teng et al. 2005; Solfanelli et al. 2006). PAP1 was demonstrated to be a major regulator of anthocyanin synthesis, as its overexpression by cauliflower mosaic virus 35S enhancer resulted in induction of anthocyanin genes and massive ectopic accumulation of anthocyanins (Borevitz et al. 2000; Tohge et al. 2005). As a result of PAP1 induction by sucrose, an artificial culturing condition consisting of 3 % sucrose and high light, termed anthocyanin induction condition or AIC, has been extensively used for the research of anthocyanin biosynthesis and trafficking (Poustka et al. 2007; Pourcel et al. 2010).
Recently, direct evidence has emerged that under drought and other oxidative stresses, plants engineered to produce high levels of anthocyanins have increased yield and antioxidant capacity compared to control plants (Nakabayashi et al. 2013; Wang et al. 2013). These observations are expected to spur the engineering of anthocyanins in crop plants for increased antioxidant capacity. In addition, unrelated efforts to engineer color into commercial, genetically modified commodities to facilitate their identification and monitoring (Kovinich et al. 2011), and anthocyanin content in foods for added health benefits (Butelli et al. 2008), underscore the importance of determining whether all anthocyanins may be considered equal in terms of their function in the plant.
Collectively, plants produce more than 500 anthocyanins with unique chemical structure (Andersen and Markham 2006), and individual anthocyanins possess different radical scavenging activity in vitro (Garcia-Alonso et al. 2005). Anthocyanins are characterized by the degree of hydroxylation or methoxylation of the anthocyanidin chromophore, and the decorations added to this backbone. For example, pelargonidin, cyanidin, and delphinidin contain one-, two- and three hydroxyl groups on the B-ring, respectively (Fig. 1a). The anthocyanidin core becomes a stable anthocyanin by the addition of a glycose (mainly glucose) at C3; however, acyl, hydroxycinnamic acid, and other moieties can be added to the backbone to yield more complex anthocyanins. It is common for plants to accumulate several different types of anthocyanins that derive from one or more anthocyanidin precursors. Arabidopsis accumulates more than 20 highly decorated derivatives of cyanidin (Tohge et al. 2005; Pourcel et al. 2010; Saito et al. 2013); the structures of those discussed in this study are illustrated in Fig. 1b.
The genes required for the biosynthesis and regulation of anthocyanins and other flavonoids are well described (Koes et al. 1994, 2005; Mol et al. 1998; Winkel-Shirley 2001; Grotewold 2006; Petroni and Tonelli 2011a; Saito et al. 2013). The inducible accumulation of anthocyanins in the mesophyll is controlled by the homeodomain transcription factor ANTHOCYANINLESS2 (Kubo et al. 1999); however, it remains to be determined whether ANTHOCYANINLESS2 activates anthocyanin biosynthetic genes directly, or causes the induction of the MYB-bHLH-WD40 ternary complex, which includes the MYB transcription factor PAP1, known to bind and activate the promoters of anthocyanin genes (Petroni and Tonelli 2011b). In Arabidopsis, the sequential order of biosynthetic steps of anthocyanin synthesis is best represented by a grid, whereby a single product of one reaction may be used as a substrate for several different reactions (Yonekura-Sakakibara et al. 2012). The enzymes that determine the pathway that intermediates traverse through the grid as well as the identity of the final products are; 5GT (a.k.a UGT75C1), BLGU10, A5GlcMalT, A3G2″XylT (a.k.a. UGT79B1), A3GlcCouT, and SAT (a.k.a. A3Glc2″XylSinT) (Tohge et al. 2005; D’Auria et al. 2007; Fraser et al. 2007; Yonekura-Sakakibara et al. 2012; Miyahara et al. 2013). Specific anthocyanins accumulate during development in an organ-specific manner in Arabidopsis (Saito et al. 2013). The synthesis of numerous structurally diverse anthocyanins and their presence in different organs may suggest that all anthocyanins may not be considered equal, and in light of this an important question remains––do different anthocyanins accumulate in response to different stress conditions?
Materials and methods
Plant materials and growth conditions
Wild-type seeds of Arabidopsis thaliana (ecotype Columbia) were surface-sterilized on a mixer wheel for five min in 70 % ethanol 0.2 % Triton X, rinsed 3 times with ethanol, dried, and planted on 0.5MS/3 % sucrose/0.5 % agar medium. After 3 days of stratification, plants were grown for 10 days under 24 h white light at 22 °C for control condition. For stress conditions, specific additives were combined with the medium, and pH adjusted to 5.8 before autoclaving. For experiments done in AIC seeds were sown in water containing 3 % sucrose and grown on a rotary shaker under the same conditions as above for 5 days.
Stress conditions and metabolite extraction
The following concentrations were applied for stress conditions: 250 mM mannitol, 100 mM NaCl, 100 mM MgSO4, low pH was adjusted to 3.3 (not completely a gel as a consequence of the low pH) and high pH to 7.3. For cold treatment, plants were transferred 8 days after germination to 4 °C, under the same light conditions, for 48 h before tissue collection. For the condition −P, 0.5MS medium was prepared using −P MS (Caisson Labs MSP11). The control was plants grown on 0.5MS under the same light and temperature conditions as the stress treatments.
Ensuring that roots did not have any residual medium, whole seedlings were collected and stored at −80 °C until lyophilization. After 2 days lyophilization, dry weight was measured and 50 µg/μl extraction solution [50 % (v/v) methanol, 3 % (v/v) formic acid] was added and incubated at room temperature overnight on a rotary shaker. Tubes were then centrifuged at 13,500g for 2 min and the supernatant was passed through 0.2 µM filters (Nanosep ODM02C35), and the filtrate analyzed by spectrophotometry and HPLC–PDA.
Total anthocyanins were measured using a spectrophotometer (Nano Drop ND-1000). Metabolite compositions were analyzed using a Waters Alliance 2695 HPLC equipped with PDA. 20 μl of plant extract was injected onto a Symmetry C18 column (4.6 × 75 mm, 100Å, 3.5 µm) held at 35 °C. The mobile phase flow rate was 1 mL min−1 and consisted of buffers A [5 % (v/v) formic acid in water] and B [5 % (v/v) formic acid in acetonitrile], with the following elution profile (0 min 100 % A, 20 min 75 % A, 22 min 20 % A, 22.1 min 100 % B, 25 min 100 % B, 25.1 min 100 % A, 32 min 100 % A) using a linear gradient between time points. Area under the peak (AU2) was determined using the manual integration option of Empower software, at 532 nm for anthocyanins, and 330 nm for SEs and flavonols. Metabolite identities were determined by LC–MS/MS as described previously (Pourcel et al. 2010). To determine the extinction coefficients of A11 and A9* relative to cyanidin, Arabidopsis anthocyanins were first purified by HPLC–PAD equipped with a Waters Fraction Collector II. The purity of isolates was validated by TLC and by HPLC–PAD monitoring at 532, 330, and 280 nm. To determine extinction coefficients, absorbances of individual compounds, exposed or not to acid hydrolysis, were compared at 530 nm, and extinction coefficient of the hydrolyzed sample was assigned the value of cyanidin in solvent 0.1 % HCl in ethanol (34700 L cm−1 mol) (Ribereau-Gayon 1959). Acid hydrolysis was conducted using seven volumes of 2:3 HCl:1-butanol for 15 min at 95 °C, compounds were lyophilized to dryness and resuspended in 0.1 % HCl in ethanol. To confirm complete hydrolysis, TLC was conducted according to Andersen and Francis (1985) using cellulose layer and the solvent system 24.9:23.7:51.4 (HCl:formic acid:water, by vol.). The commercial standards cyanidin and cyanidin 3-O-glucoside were used as controls.
Cluster analysis was performed with Multiexperiment Viewer software Version 4.9 using default parameters and the Euclidean Distance metric. Metabolite profiles were obtained as described above. Gene expression data were obtained from the Bio-Analytic Resource (http://www.bar.utoronto.ca/efp).
Results and discussion
Anthocyanin induction by different abiotic stress conditions
Anthocyanins are commonly reported as being induced by abiotic stress. However, the level of induction of anthocyanins across different stresses is unknown. To determine the response of Arabidopsis from the perspective of anthocyanin accumulation, we grew Arabidopsis under seven physiologically extreme stress conditions previously reported to trigger anthocyanin accumulation, and the levels of total anthocyanin were quantified by spectrophotometry at 532 nm (Fig. 2). For reference, we also included seedlings grown for 5 days in AIC, an artificial liquid culture condition that does not represent a natural physiological stress, but is well characterized for inducing high levels of anthocyanins (Poustka et al. 2007; Pourcel et al. 2010). Our results show that seedlings grown on the 0.5MS control condition for 10 days exhibited some low-level anthocyanin pigmentation, similar to that reported previously for 3-day-old Arabidopsis seedlings (Shirley et al. 1995). Relative to the control, deficiency of the macronutrient phosphate (−P) and low pH medium (pH 3.3) resulted in significant induction of total anthocyanin levels, similar to AIC (Fig. 2). It is noteworthy that AIC media contains 3 % sucrose, similar to the control media, but lacks other nutrients such as a nitrogen source, which has been shown to further enhance the accumulation of anthocyanins (Hsieh et al. 1998). Under our experimental conditions, 100 mM NaCl or 100 mM MgSO4 did not result in a statistically significant change in the levels of total anthocyanin. This contrasts the induction of anthocyanins observed in 7-day-old tomato and red cabbage seedlings after the application of 100 mM NaCl through a hydroponic system (Eryılmaz 2006), and may simply be due to reduced uptake of salts from our agar-based media, or to adaptation to the stress over longer-term exposure, rather than different responses among species. Unexpectedly, 250 mM mannitol resulted in a statistically significant (P < 0.05, two sided t test) reduction in total anthocyanins, as did pH 7.3. High pH has also been shown to reduce total anthocyanin levels in grape cell cultures (Suzuki 1995). By contrast, seedlings grown in pH 7.3 medium had unchanged levels of flavonols and SEs, as indicated by the absence of a change in the absorbance at 330 nm, whereas growth in mannitol led to a reduction in both flavonol and SE absorbance (Supplemental Fig. S1). Overall, our results demonstrate that similar to AIC, low pH and phosphate deficiency induce anthocyanin accumulation, whereas osmotic stress with mannitol and high pH promoted a reduction in total anthocyanins.
Unique anthocyanin profiles result from different stress conditions
Arabidopsis synthesizes more than 21 anthocyanins when cultured in AIC or when overexpressing the transcription factor PAP1 (Tohge et al. 2005; Rowan et al. 2009; Pourcel et al. 2010; Saito et al. 2013). Our results show that, under our experimental growth conditions, some stress conditions previously shown to induce anthocyanin pigmentation (e.g., salt, cold) did not result in significantly elevated total levels of anthocyanins (Fig. 2). Previous studies suggested that Arabidopsis anthocyanins may have different rates of catabolism (Rowan et al. 2009), hence, similar levels of anthocyanins could be a consequence of some being induced by a particular stress condition, while others are catabolized, or their synthesis repressed. This raises the question of whether specific anthocyanins may be preferentially induced, or repressed, in response to a particular stress.
To answer this question, we conducted HPLC–PAD on aqueous methanol extracts from seedlings grown under the seven stress conditions. Again, AIC was used as a reference. Interestingly, different stresses were found to lead to distinct anthocyanin profiles (Fig. 3). Under control growth conditions, only four anthocyanins were detected: A8, A9*, A11* and A11 (Fig. 3a). A11, the most decorated anthocyanin, was the predominant anthocyanin, similar to that reported previously for wild-type Arabidopsis seedlings grown on agar- or vermiculite-based media (Tohge et al. 2005; Rowan et al. 2009). For seedlings grown on 100 mM MgSO4, we observed the de novo accumulation of A5*/A9, A5, A7, and A3, in addition to a moderate (less than tenfold) increase of the four anthocyanins found in the control (Fig. 3d). The de novo induction of several anthocyanin compounds in the MgSO4 condition was accompanied by a circa 10 % decrease in the relative proportions of A11 and A11* in the total anthocyanin content, compared to the control (compare Fig. 3h to Fig. 3e). In contrast, the proportions of A11 and A11* were 10 % greater and 10 % less, respectively, in the pH 3.3 condition compared to the control (compare Fig. 3f to Fig. 3e). The pH 3.3 condition also led to the de novo induction of A5*/A9, A5, A7, and A3, in addition to A8*, an anthocyanin not found in the MgSO4 condition. AIC had roughly 10- and 1.5-fold less induction of A11 compared to pH 3.3 and MgSO4, respectively. Seedlings grown under AIC had more than 80-fold increase of A9* compared to the control (Fig. 3c). A calculation of extinction coefficients (absorptivities) of purified A11 (61,300 L cm−1 mol) and A9* (53,400 L cm−1 mol) indicated that the induction by AIC rendered A9* levels equivalent to A11. AIC also resulted in a significant induction of A5 (P < 0.05, two sided t test), which was absent from the control and is generally not highly induced by the other stress conditions, and was the only condition that accumulated detectable levels of A3 (Supplemental Fig. S2). Overall, these results show that different stress conditions lead to unique anthocyanin profiles.
Similar anthocyanin fingerprints for similar physiological stresses
As different subsets of anthocyanins showed different accumulation profiles in response to particular stresses, our next objective was to determine whether anthocyanin profiles could be used to identify similarities among stress responses, with the ultimate goal of establishing whether anthocyanin profiles can provide a “fingerprint” of the stress status of plants. To test this hypothesis, we performed hierarchical clustering using anthocyanin profiles (Fig. 4a). As expected, the anthocyanin profiles of control samples clustered with those grown in pH 7.3 medium. These two conditions led to the accumulation of the same four anthocyanins but with different relative amounts of the individual species. Osmotic stress (mannitol) clustered with NaCl. This is consistent with the fact that osmotic stress is a significant component of the overall stress caused by excess sodium ions (Hasegawa et al. 2000). Interestingly, MgSO4 clustered more closely with cold and AIC, than to NaCl. Roughly, ten percent of the genes up-regulated by cold were also found to be up-regulated by high salinity in a microarray containing about 7,000 independent full-length cDNAs (Seki et al. 2002). However, cold has been shown to induce anthocyanin synthesis and MgSO4 has been shown to stabilize anthocyanins (Shaked-Sachray et al. 2002), so the similarities in anthocyanin profiles in this case may be due to different mechanisms. Anthocyanin profiles from low pH (pH 3.3) and phosphate deficient conditions cluster together. This is consistent with the fact that phosphate in the medium becomes insoluble at low pH, and thus cannot be taken up by the plant (Hoeft et al. 2000). Notably, the −P and low pH treatments form a subcluster that is distinct from the other osmotic and high salinity stresses. Taken together, these results demonstrate that similar anthocyanin fingerprints are induced by related physiological stress conditions.
Stress-induced versus constitutive anthocyanins
The hierarchical clustering of the different anthocyanins across stresses showed that A11 is a unique outlier (Fig. 4a). A11 accumulated to relatively high levels even in the absence of abiotic stress. The cluster containing A8, A9*, and A11* accumulated in stress and non-stressed conditions, and generally was induced most highly by stress. Members of the final cluster, comprised of A3, A5, A5*/A9, A7, and A8*, were exclusively induced by stress. These results show that there exists both stress inducible and constitutive (or developmentally induced) anthocyanin populations in Arabidopsis.
Subsets of anthocyanins are similarly induced by a range of stress conditions
In light of the fact that stress conditions preferentially induce specific anthocyanins, we wanted to determine whether specific anthocyanin compounds show similar induction profiles across stress conditions, as this may suggest similar functional demand for particular sets of anthocyanins during stress, and/or co-induction of specific steps in anthocyanin biosynthesis. An analysis of the relative levels of single anthocyanins across the different stresses demonstrated that A8 had similar relative accumulation profiles as A11, with maximum levels found in seedlings deprived of phosphate and seedlings exposed to low pH (Fig. 5a, b). By contrast, A5 and A9* exhibited similar induction profiles, distinct from those of A8 and A11, with maximum levels found in AIC and −P (Fig. 5c, d). These two sets of anthocyanins differ in structure by the presence or absence of the glucose moiety attached to the coumaryl at position C3-6″ (position R2 in Fig. 1). The enzyme that catalyzes the addition of this glucose was recently identified to be the acyl-glucose-dependent glucosyltransferase, BGLU10 (Miyahara et al. 2013).
Anthocyanin biosynthesis is believed to be controlled primarily at the level of transcription of the genes encoding biosynthetic enzymes (Koes et al. 2005; Tohge et al. 2005; Quattrocchio et al. 2006; Petroni and Tonelli 2011a). To determine whether the coordinated induction of anthocyanins by stress may be explained by co-induction of gene transcripts, we performed hierarchical cluster analysis of anthocyanin gene expressions across salt, drought, and cold stress conditions, using datasets available from the Bio-Analytic Resource (BAR) for Plant Biology (http://bar.utoronto.ca). The enzymes for anthocyanin modification are depicted in Fig. 4c. The cluster analysis shows that 5GT and A3GlcCouT were outliers, and were the most constitutively expressed genes under these stresses (Fig. 4b), suggesting these enzymes may not be responsible for the dissimilar induction profiles of A5 and A9* versus A8 and A11 (Fig. 5), however, it is worth noting that the stress conditions used for gene expression analysis and those for metabolite analysis were not identical. A3G2″XylT and A5GlcMalT formed a subcluster, as their induction was less frequent than the other anthocyanin modification enzymes, but most commonly coordinated. SAT was less frequently co-induced with A3G2″XylT and A5GlcMalT, and BGLU10 was even less frequently co-induced with SAT. The infrequent coordinated induction of BGLU10 with other anthocyanin genes during stress is consistent with A5 and A9* (labeled green in Fig. 4c) having induction profiles similar to each other (Fig. 5), but different than A8 and A11 (Fig. 5, metabolites labeled red in Fig. 4c), as the transfer of glucose to position C3-6″ is the biosynthetic step that separates these two anthocyanin groups (Fig. 4c).
Anthocyanins are specialized metabolites that often accumulate in vegetative tissues when plants are subjected to different types of stress conditions (Hatier and Gould 2008). Whether (and how) anthocyanins function as stress protective molecules have been a subject of debate (Gould 2004b); the most parsimonious explanation being that they play a role in protecting against the ROS that often accumulate during stress. Our studies show that Arabidopsis often responds to different types of abiotic stress conditions not only by increasing total levels of anthocyanins, but also by altering the profiles of anthocyanin accumulation (Figs. 3 and 4). Indeed, while some of the profile changes are likely a consequence of alterations in the degradation of some anthocyanins (e.g., MgSO4), changes in the expression of genes encoding many of the enzymes involved in the decoration of anthocyanins suggest that most of the effects are transcriptional. These results support that plants can preferentially synthesize different anthocyanins in response to distinct stresses, suggesting that the various decoration patterns on an anthocyanin backbone actually impart a function favorable in a particular stress condition.
Anthocyanin 3-O-glucoside: 2″-O-xylosyltransferase
Anthocyanin 3-O-glucoside: 6″-O-p-coumaroyltransferase
Anthocyanin inductive condition
Anthocyanin 3-O-6″-coumaroylglucoside: glycosyltransferase
High performance liquid chromatography–photodiode array
Liquid chromatography–tandem mass spectrometry
Murashige and Skoog
Production of anthocyanin pigment 1
Reactive oxygen species
Agati G, Azzarello E, Pollastri S, Tattini M (2012) Flavonoids as antioxidants in plants: location and functional significance. Plant Sci 196:67–76
Andersen OM, Francis GW (1985) Simultaneous analysis of anthocyanins and anthocyanidins on cellulose thin layers. J Chromatogr 318:450–454
Andersen OM, Markham KR (2006) Flavonoids: chemistry, biochemistry and applications. CRC Taylor & Francis, Boca Raton, pp 471–537
Basu S, Roychoudhury A, Saha PP, Sengupta DN (2010) Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regul 60:51–59
Borevitz JO, Xia Y, Blount J, Dixon RA, Lamb C (2000) Activation tagging identifies a conserved MYB regulatory of phenylpropanoid biosynthesis. Plant Cell 12:2383–2393
Butelli E, Titta L, Giorgio M, Mock HP, Matros A, Peterek S, Schijlen EG, Hall RD, Bovy AG, Luo J, Martin C (2008) Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat Biotechnol 26:1301–1308
Chalker-Scott L (1999) Environmental significance of anthocyanins in plant stress responses. Photochem Photobiol 70:1–9
Christie PJ, Alfenito MR, Walbot V (1994) Impact of low-temperature stress on general phenylpropanoid and anthocyanin pathways: enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings. Planta 194:541–549
Crifò T, Puglisi I, Petrone G, Recupero GR, Lo Piero AR (2011) Expression analysis in response to low temperature stress in blood oranges: implication of the flavonoid biosynthetic pathway. Gene 476:1–9
D’Auria JC, Reichelt M, Luck K, Svatoš A, Gershenzon J (2007) Identification and characterization of the BAHD acyltransferase malonyl CoA: anthocyanidin 5-O-glucoside-6″-O-malonyltransferase (At5MAT) in Arabidopsis thaliana. FEBS Lett 581:872–878
Eryılmaz F (2006) The relationships between salt stress and anthocyanin content in higher plants. Biotechnol Biotechnol Equip 20:47–52
Fraser CM, Thompson MG, Shirley AM, Ralph J, Schoenherr JA, Sinlapadech T, Hall MC, Chapple C (2007) Related Arabidopsis serine carboxypeptidase-like sinapoylglucose acyltransferases display distinct but overlapping substrate specificities. Plant Physiol 144:1986–1999
Garcia-Alonso M, Rimbach G, Sasai M, Nakahara M, Matsugo S, Uchida Y, Rivas-Gonzalo JC, Pascual-Teresa D (2005) Electron spin resonance spectroscopy studies on the free radical scavenging activity of wine anthocyanins and pyranoanthocyanins. Mol Nutr Food Res 49:1112–1119
Gould KS (2004a) Nature’s Swiss army knife: the diverse protective roles of anthocyanins in leaves. BioMed Res Int 2004:314–320
Gould KS (2004b) Nature’s Swiss army knife: the diverse protective roles of anthocyanins in leaves. J Biomed Biotechnol 2004:314–320
Gould K, McKelvie J, Markham K (2002) Do anthocyanins function as antioxidants in leaves? Imaging of H2O2 in red and green leaves after mechanical injury. Plant Cell Environ 25:1261–1269
Grotewold E (2006) The genetics and biochemistry of floral pigments. Annu Rev Plant Biol 57:761–780
Hasegawa PM, Bressan RA, Zhu J-K, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Biol 51:463–499
Hatier JH, Gould KS (2008) Foliar anthocyanins as modulators of stress signals. J Theor Biol 253:625–627
Hatier J-HB, Gould KS (2009) Anthocyanin function in vegetative organs. In: Winefield C, Davies K, Gould KS (eds) Anthocyanins: biosynthesis, functions and applications. Springer, New York, pp 1–19
Hoeft RG, Nafziger ED, Johnson RR, Aldrich SR (2000) Modern corn and soybean production. MCSP Publications, Champaign, pp 107–171
Hsieh MH, Lam HM, Van De Loo FJ, Coruzzi G (1998) A PII-like protein in Arabidopsis: putative role in nitrogen sensing. Proc Natl Acad Sci USA 95:13965–13970
Jiang C, Gao X, Liao L, Harberd NP, Fu X (2007) Phosphate starvation root architecture and anthocyanin accumulation responses are modulated by the gibberellin-DELLA signaling pathway in Arabidopsis. Plant Physiol 145:1460–1470
Koes RE, Quattrocchio F, Mol JN (1994) The flavonoid biosynthetic pathway in plants: function and evolution. BioEssays 16:123–132
Koes R, Verweij W, Quattrocchio F (2005) Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. Trends Plant Sci 10:236–242
Kovinich N, Saleem A, Rintoul TL, Brown DC, Arnason JT, Miki B (2011) Coloring genetically modified soybean grains with anthocyanins by suppression of the proanthocyanidin genes ANR1 and ANR2. Transgenic Res 21:757–771
Kubo H, Peeters AJ, Aarts MG, Pereira A, Koornneef M (1999) ANTHOCYANINLESS2, a homeobox gene affecting anthocyanin distribution and root development in Arabidopsis. Plant Cell 11:1217–1226
Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
Miyahara T, Sakiyama R, Ozeki Y, Sasaki N (2013) Acyl-glucose-dependent glucosyltransferase catalyzes the final step of anthocyanin formation in Arabidopsis. J Plant Physiol 170:619–624
Mizukami H, Nakamura M, Tomita K, Higuchi K, Ohashi H (1991) Effect of macronutrients on anthocyanin production in roselle (Hibiscus sabdariffa L.) callus cultures. Plant Tiss Cult Lett 8:14–20
Mol J, Grotewold E, Koes R (1998) How genes paint flowers and seeds. Trends Plant Sci 3:212–217
Nakabayashi R, Yonekura-Sakakibara K, Urano K, Suzuki M, Yamada Y, Nishizawa T, Matsuda F, Kojima M, Sakakibara H, Shinozaki K (2013) Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids. Plant J. doi:10.1111/tpj.12388
Parkin J (1903) On the localisation of anthocyanin (red-cell sap) in foliage leaves. Rep Br Ass Adv Sci 73:862
Petroni K, Tonelli C (2011a) Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Sci 181:219–229
Petroni K, Tonelli C (2011b) Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Sci 181:219–229
Pourcel L, Irani NG, Lu Y, Riedl K, Schwartz S, Grotewold E (2010) The formation of anthocyanic vacuolar inclusions in Arabidopsis thaliana and implications for the sequestration of anthocyanin pigments. Mol Plant 3:78–90
Poustka F, Irani NG, Feller A, Lu Y, Pourcel L, Frame K, Grotewold E (2007) A trafficking pathway for anthocyanins overlaps with the endoplasmic reticulum-to-vacuole protein-sorting route in Arabidopsis and contributes to the formation of vacuolar inclusions. Plant Physiol 145:1323–1335
Quattrocchio F, Baudry A, Lepiniec L, Grotewold E (2006) The regulation of flavonoid biosynthesis. In: Grotewold E (ed) The science of flavonoids. Springer, New York, pp 97–122
Rajendran L, Ravishankar G, Venkataraman L, Prathiba K (1992) Anthocyanin production in callus cultures of Daucus carota as influenced by nutrient stress and osmoticum. Biotechnol Lett 14:707–712
Rhoads DM, Umbach AL, Subbaiah CC, Siedow JN (2006) Mitochondrial reactive oxygen species. Contribution to oxidative stress and interorganellar signaling. Plant Physiol 141:357–366
Ribereau-Gayon P (1959) Recherches sur les anthocyannes des vegetaux. Application au genre Vitis. Doctoral dissertation. University of Bordeaux. Libraire Generate de I’Ensignement, Paris, France, p 118
Rowan DD, Cao M, Lin-Wang K, Cooney JM, Jensen DJ, Austin PT, Hunt MB, Norling C, Hellens RP, Schaffer RJ (2009) Environmental regulation of leaf colour in red 35S: PAP1 Arabidopsis thaliana. New Phytol 182:102–115
Saito K, Yonekura-Sakakibara K, Nakabayashi R, Higashi Y, Yamazaki M, Tohge T, Fernie AR (2013) The flavonoid biosynthetic pathway in Arabidopsis: structural and genetic diversity. Plant Physiol Biochem 72:21–34
Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J 31:279–292
Shaked-Sachray L, Weiss D, Reuveni M, Nissim-Levi A, Oren-Shamir M (2002) Increased anthocyanin accumulation in aster flowers at elevated temperatures due to magnesium treatment. Physiol Plant 114:559–565
Shirley BW, Kubasek WL, Storz G, Bruggemann E, Koornneef M, Ausubel FM, Goodman HM (1995) Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis. Plant J 8:659–671
Solfanelli C, Poggi A, Loreti E, Alpi A, Perata P (2006) Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis. Plant Physiol 140:637–646
Sperdouli I, Moustakas M (2012) Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress. J Plant Physiol 169:577–585
Suzuki M (1995) Enhancement of anthocyanin accumulation by high osmotic stress and low pH in grape cells (Vitis hybrids). J Plant Physiol 147:152–155
Teng S, Keurentjes J, Bentsink L, Koornneef M, Smeekens S (2005) Sucrose-specific induction of anthocyanin biosynthesis in Arabidopsis requires the MYB75/PAP1 gene. Plant Physiol 139:1840–1852
Tohge T, Nishiyama Y, Hirai MY, Yano M, Nakajima J, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K (2005) Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor. Plant J 42:218–235
Wang H, Fan W, Li H, Yang J, Huang J, Zhang P (2013) Functional characterization of dihydroflavonol-4-reductase in anthocyanin biosynthesis of purple sweet potato underlies the direct evidence of anthocyanins function against abiotic stresses. PLoS One 8:e78484
Winkel BS (2004) Metabolic channeling in plants. Annu Rev Plant Biol 55:85–107
Winkel-Shirley B (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol 126:485–493
Winkel-Shirley B (2002) Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol 5:218–223
Yonekura-Sakakibara K, Fukushima A, Nakabayashi R, Hanada K, Matsuda F, Sugawara S, Inoue E, Kuromori T, Ito T, Shinozaki K, Wangwattana B, Yamazaki M, Saito K (2012) Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana. Plant J 69:154–167
Zhang W, Furusaki S (1997) Regulation of anthocyanin synthesis in suspension cultures of strawberry cell by pH. Biotechnol Lett 19:1057–1061
This project was supported by National Science Foundation grant MCB-1048847 to EG and MSO. NK was supported by the Pelotonia Postdoctoral Fellowship Program and GK was supported by the NIH Postbacculaureate Research Education Program (PREP) Grant R25 GM089571.
Special topic: Anthocyanins. Guest editor: Stefan Martens.
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Supplemental Fig. S1 Amount of total flavonols and sinapate esters produced by Arabidopsis after various stress conditions. Plants were cultured under stress conditions, tissues were extracted, and metabolites analyzed as described in the Materials and Methods. Error bars represent standard error of the mean (n = 3). aLess than control, bgreater than control, P < 0.05; two-tailed Student’s t test (PDF 18 kb)
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Kovinich, N., Kayanja, G., Chanoca, A. et al. Not all anthocyanins are born equal: distinct patterns induced by stress in Arabidopsis . Planta 240, 931–940 (2014). https://doi.org/10.1007/s00425-014-2079-1
- Abiotic stress
- Anthocyanin pigmentation