Abstract
Regenerates were obtained following somatic hybridization between tall wheatgrass (Agropyron elongatum) and bread wheat (Triticum aestivum cv. Jinan177) protoplasts. Two lines (CU and XI) were self-fertile in the first (R0) and subsequent (R1 and R2) generations. The phenotype of each R1 population was uniform. All CU progeny were phenotypically similar to the tall wheatgrass parent, while XI progeny had thinner, smoother and softer leaves. Cytological analysis showed that more wheat chromatin was present in the hybrid callus than in the R1 and R2 plants, and that some intercalary translocations of wheat chromosome segments were retained in the R2 generation. AFLP profiling confirmed the presence of wheat DNA in the introgression lines. Analysis of the high molecular weight glutenin subunit content of derived seed identified three novel subunits, not present in either the wheat or the tall wheatgrass parent. Microsatellite-based profiling of the chloroplast genome of the introgression lines suggested that only chloroplast sequences from the tall wheatgrass parent were present. The specifically inherited phenomena and possible application of these hybrids are discussed.
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Abbreviations
- AFLP:
-
Amplification fragment length polymorphism
- GISH:
-
Genome in situ hybridization
- HMW-GS:
-
High-molecular-weight glutenin subunit
References
Altuntepe MD, Jauhar PP (2001) Production of durum wheat substitution haploids from durum × maize crosses and their cytological characterization. Genome 44:137–142
Babiychuk E, Kushnir S, Gleba YY (1992) Spontaneous extensive chromosome elimination in somatic hybrids between somatically congruent species Nicotiana tabacum L. and Atropa belladonna L. Theor Appl Genet 84:87–91
Buiteveld J, Kassies W, Geels R, Lookeren-Campagne MM, Jacobsen E, Creemers-Molenaar J (1998) Biased chloroplast and mitochondrial transmission in somatic hybrids of Allium ampeloprasum L. and Allium cepa L. Plant Sci 131:219–228
Chen SY, Liu SW, Xu CH, Chen YZ, Xia GM (2004a) Heredity of chloroplast and nuclear genomes of asymmetric somatic hybrid lines between wheat and couch grass. Acta Bot Sin 46:110–115
Chen SY, Xia GM, Quan TY, Xiang FN, Jin Y, Chen HM (2004b) Introgression of salt-tolerance from somatic hybrids between common wheat and Thinopyrum ponticum. Plant Sci 167:773–779
Doyle JJ, Doyle JI (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Feldman M, Sears ER (1981) The wild gene resources of wheat. Scientific American January:98–101
Feng DS, Xia GM, Zhao SY, Chen FG (2004) Two quality-associated HMW glutenin subunits in a somatic hybrid line between Triticum aestivum and Agropyron elongatum. Theor Appl Genet 110:136–144
Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87
Gernand D, Rutten T, Varshney A, Rubtsova M, Prodanovic S, Brüß C, Kumlehn J, Matzk F, Houben A (2005) Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation. Plant Cell 17:2431–2438
Hu YK, Zhao Q, Liu H, Zhang YS (2001) Studies on establishing great greenbelt on arid land using Agropyron elongatum. Grassland and Turf 93:27–29
Jauhar PP (1995) Meiosis and fertility of F1 hybrids between hexaploid bread wheat and decaploid tall wheatgrass (Thinopyrum ponticum). Theor Appl Genet 90:865–871
Jauhar PP, Chibbar RN (1999) Chromosome-mediated and direct gene transfers in wheat. Genome 42:570–583
Liu SW, Zhao SY, Chen FG, Xia GM (2007) Generation of novel high quality HMW-GS genes in two introgression lines of Triticum aestivum/Agropyron elongatum. BMC Evol Biol 7:76
Panayotov I (1983) The cytoplasm in Triticinae. In: Sakamoto S (ed) Proceedings of 6th International wheat genetics symposium, pp 481–497
Perez-Jones A, Mallory-Smith CA, Hansen JL, Zemetra RS (2006) Introgression of an imidazolinone-resistance gene from winter wheat (Triticum aestivum L.) into jointed goatgrass (Aegilops cylindrica Host). Theor Appl Genet 114:177–186
Riddle NC, Birchler JA (2003) Effects of reunited diverged regulatory hierarchies in allopolyploids and species hybrids. Trends Genet 19:597–600
Schoenenberger N, Guadagnuolo R, Bianchi DS, Küpfer P, Felber F (2006) Molecular analysis, cytogenetics and fertility of introgression lines from transgenic wheat to Aegilops cylindrica Host. Genetics 174:2061–2070
Sears ER (1993) Use of radiation to transfer alien chromosome segments to wheat. Crop Sci 33:897–901
Shan L, Zhao SY, Xia GM (2006) Research progress on the identification of salt-tolerance related genes and molecular mechanism on salt tolerance in higher plants. Mol Plant Breed 14:15–22
Skarzhinskaya M, Landgren M, Glimelius K (1996) Production of intertribal somatic between Brassica napus L. and Lesquerella fendleri (Gray) Wats. Theor Appl Genet 93:1242–1250
Takamizo T, Spangenberg G, Suginobu KI, Potrykus I (1991) Intergeneric somatic hybridization in Gramineae: somatic hybrid plants between tall fescue (Festuca arundinacea Schreb.) and Italian ryegrass (Lolium multiflorum Lam.). Mol Gen Genet 231:1–6
Vos P, Hogers R, Bleeker M (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Wang J, Xiang FN, Xia GM (2005) Agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line. Planta 221:277–286
Xia GM, Chen HM (1996) Plant regeneration from intergeneric somatic hybridization between Triticum aestivum and Leymus chinensis. Plant Sci 120:197–203
Xia GM, Xiang FN, Zhou AF, Wang H, Chen HM (2003) Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet 107:299–305
Xiang FN, Xia GM, Chen HM (2003) Effect of UV dosage on somatic hybridization between common wheat (Triticum aestivum L.) and Avena sativa L. Plant Sci 164:697–707
Xiang FN, Xia GM, Zhi DY, Wang J, Nie H, Chen MH (2004) Regeneration of somatic hybrids in relation to the nuclear and cytoplasmic genomes of wheat and Setaria italica. Genome 47:680–688
Xu CH, Xia GM, Zhi DY, Xiang FN, Chen HM (2003) Integration of maize nuclear and mitochondrial DNA into the wheat genome through somatic hybridization. Plant Sci 169:1001–1008
Zhao Q, Lu ZY, Su SQ, Shen SY, Chen AL, Han QD (1996a) Studies on improving saline—alkali land and establishing artificial grassland using Agropyron elongatum. Grass-feeding Livestock 93:48–49
Zhao Q, Shen SY, Ren ZC, Lu ZY, Su SQ, Shi GQ (1996b) Studies on breeding effect of Agropyron elongatum. Grass-feeding Livestock 91:35–37
Zhong GZ, Mu SM, Zhang ZB (2002) Study on remote hybridization of wheat. Chinese Science, Beijing, pp 92–97
Zhou AF, Xia GM (2005) Introgression of the Haynaldia villosa genome into γ-ray-induced asymmetric somatic hybrids of wheat. Plant Cell Rep 24:289–296
Acknowledgments
This work was supported by the funds of the Major Program of the Natural Science Foundation of China (No. 30530480), the National Basic Research 973 Program of China (2006CB100100), the Science Foundation of Shandong Province (No. Q2006D02), and the National Key Technology R&D Program (No. 2007BAD59B06).
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Haifeng Cui and Zhiyong Yu were contributed equally to this article.
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Cui, H., Yu, Z., Deng, J. et al. Introgression of bread wheat chromatin into tall wheatgrass via somatic hybridization. Planta 229, 323–330 (2009). https://doi.org/10.1007/s00425-008-0832-z
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DOI: https://doi.org/10.1007/s00425-008-0832-z