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NbLRK1, a lectin-like receptor kinase protein of Nicotiana benthamiana, interacts with Phytophthora infestans INF1 elicitin and mediates INF1-induced cell death

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Abstract

Phytophthora infestans INF1 elicitin causes the hypersensitive response (HR) in Nicotiana benthamiana (Kamoun et al. in Plant Cell 10:1413–1425, 1998). To identify N. benthamiana proteins that interact with INF1, we carried out a yeast two-hybrid screen. This screen resulted in the isolation of a gene NbLRK1 coding for a novel lectin-like receptor kinase. NbLRK1 interacted with INF1 through its VIb kinase subdomain. Purified INF1 and NbLRK1 proteins also interacted in vitro. INF1 treatment of N. benthamiana leaves induced autophosphorylation of NbLRK1. Most importantly, virus-induced gene silencing (VIGS) of NbLRK1 delayed INF1-mediated HR in N. benthamiana. These data suggest that NbLRK1 is a component of the N. benthamiana protein complex that recognizes INF1 elicitor and transduces the HR signal.

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Abbreviations

HR:

Hypersensitive response

LRK:

Lectin-like receptor kinase

MBP:

Myelin basic protein

VIGS:

Virus-induced gene silencing

Y2H:

Yeast two-hybrid assay

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Acknowledgments

We acknowledge David Baulcombe, Sainsbury Laboratory, John Innes Center, for pPC2S and PTV:00 and Nam Hai Chua, Rockefeller University, for pTA7001. This work was carried out in part by support from “Program for Promotion of Basic Research Activities for Innovative Biosciences” (Japan), “Iwate University twenty-first Century COE Program: Establishment of Thermo-Biosystem Research Program” and Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation PMI-0010) to RT. We thank Matt Shenton, IBRC, for the improvement of the manuscript.

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Authors and Affiliations

  1. Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan

    Hiroyuki Kanzaki, Hiromasa Saitoh, Yoshihiro Takahashi, Thomas Berberich, Akiko Ito & Ryohei Terauchi

  2. The United Graduate School of Agricultural Sciences, Iwate University, 3-18-8, Ueda, Morioka, Iwate, 020-8550, Japan

    Ryohei Terauchi

  3. The Sainsbury Laboratory, John Innes Centre, Norwich, UK

    Sophien Kamoun

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  1. Hiroyuki Kanzaki
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  2. Hiromasa Saitoh
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  3. Yoshihiro Takahashi
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  4. Thomas Berberich
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  5. Akiko Ito
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  6. Sophien Kamoun
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  7. Ryohei Terauchi
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Correspondence to Ryohei Terauchi.

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Kanzaki, H., Saitoh, H., Takahashi, Y. et al. NbLRK1, a lectin-like receptor kinase protein of Nicotiana benthamiana, interacts with Phytophthora infestans INF1 elicitin and mediates INF1-induced cell death. Planta 228, 977–987 (2008). https://doi.org/10.1007/s00425-008-0797-y

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