NbLRK1, a lectin-like receptor kinase protein of Nicotiana benthamiana, interacts with Phytophthora infestans INF1 elicitin and mediates INF1-induced cell death
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Phytophthora infestans INF1 elicitin causes the hypersensitive response (HR) in Nicotiana benthamiana (Kamoun et al. in Plant Cell 10:1413–1425, 1998). To identify N. benthamiana proteins that interact with INF1, we carried out a yeast two-hybrid screen. This screen resulted in the isolation of a gene NbLRK1 coding for a novel lectin-like receptor kinase. NbLRK1 interacted with INF1 through its VIb kinase subdomain. Purified INF1 and NbLRK1 proteins also interacted in vitro. INF1 treatment of N. benthamiana leaves induced autophosphorylation of NbLRK1. Most importantly, virus-induced gene silencing (VIGS) of NbLRK1 delayed INF1-mediated HR in N. benthamiana. These data suggest that NbLRK1 is a component of the N. benthamiana protein complex that recognizes INF1 elicitor and transduces the HR signal.
KeywordsAutophosphorylation Hypersensitive response INF1 NbLRK1 Nicotiana Virus-induced gene silencing Y2H
Lectin-like receptor kinase
Myelin basic protein
Virus-induced gene silencing
Yeast two-hybrid assay
We acknowledge David Baulcombe, Sainsbury Laboratory, John Innes Center, for pPC2S and PTV:00 and Nam Hai Chua, Rockefeller University, for pTA7001. This work was carried out in part by support from “Program for Promotion of Basic Research Activities for Innovative Biosciences” (Japan), “Iwate University twenty-first Century COE Program: Establishment of Thermo-Biosystem Research Program” and Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation PMI-0010) to RT. We thank Matt Shenton, IBRC, for the improvement of the manuscript.
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