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Expression of calcium-activated chloride channels Ano1 and Ano2 in mouse taste cells

  • Sensory physiology
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Abstract

Specialized Ca2+-dependent ion channels ubiquitously couple intracellular Ca2+ signals to a change in cell polarization. The existing physiological evidence suggests that Ca2+-activated Cl− channels (CaCCs) are functional in taste cells. Because Ano1 and Ano2 encode channel proteins that form CaCCs in a variety of cells, we analyzed their expression in mouse taste cells. Transcripts for Ano1 and Ano2 were detected in circumvallate (CV) papillae, and their expression in taste cells was confirmed using immunohistochemistry. When dialyzed with CsCl, taste cells of the type III exhibited no ion currents dependent on cytosolic Ca2+. Large Ca2+-gated currents mediated by TRPM5 were elicited in type II cells by Ca2+ uncaging. When TRPM5 was inhibited by triphenylphosphine oxide (TPPO), ionomycin stimulated a small but resolvable inward current that was eliminated by anion channel blockers, including T16Ainh-A01 (T16), a specific Ano1 antagonist. This suggests that CaCCs, including Ano1-like channels, are functional in type II cells. In type I cells, CaCCs were prominently active, blockable with the CaCC antagonist CaCCinh-A01 but insensitive to T16. By profiling Ano1 and Ano2 expressions in individual taste cells, we revealed Ano1 transcripts in type II cells only, while Ano2 transcripts were detected in both type I and type II cells. P2Y agonists stimulated Ca2+-gated Cl− currents in type I cells. Thus, CaCCs, possibly formed by Ano2, serve as effectors downstream of P2Y receptors in type I cells. While the role for TRPM5 in taste transduction is well established, the physiological significance of expression of CaCCs in type II cells remains to be elucidated.

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Acknowledgments

We thank Heinz Breer and Peter Mombaerts for providing OMP-GFP mice. This work was supported by the Russian Foundation for Basic Research (grants 13-04-00913a, 13-04-40082H, and 14-04-91157a).

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    Authors and Affiliations

    1. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

      Alexander P. Cherkashin, Alisa S. Kolesnikova, Michail V. Tarasov, Olga A. Rogachevskaja, Marina F. Bystrova & Stanislav S. Kolesnikov

    2. Department of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, Spitalgasse 4, A-1090, Vienna, Austria

      Roman A. Romanov

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    1. Alexander P. Cherkashin
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    2. Alisa S. Kolesnikova
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    Correspondence to Stanislav S. Kolesnikov.

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    Alexander P. Cherkashin and Alisa S. Kolesnikova contributed equally to this work.

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    Cherkashin, A.P., Kolesnikova, A.S., Tarasov, M.V. et al. Expression of calcium-activated chloride channels Ano1 and Ano2 in mouse taste cells. Pflugers Arch - Eur J Physiol 468, 305–319 (2016). https://doi.org/10.1007/s00424-015-1751-z

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