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Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise

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Abstract

It has been well demonstrated that the principal factor responsible for oxidative damage during exercise is the increase in oxygen consumption. However, other theoretical factors (acidosis, catecholamine autoxidation, ischemia-reperfusion syndrome, etc.) that are known to induce, in vitro, oxidative damage may also be operative during short-term supramaximal anaerobic exercise. Therefore, we hypothesized that short-term supramaximal anaerobic exercise (30-s Wingate test) could induce an oxidative stress. Lipid peroxidation markers [serum lipid radical production detected by electron spin resonance (ESR) spectroscopy and plasma malondialdehyde (MDA) levels detected by the thiobarbituric acid reactive substances (TBARS) method], as well as erythrocyte antioxidant enzyme activities [glutathione peroxidase (GPx), superoxide dismutase (SOD)] and erythrocyte glutathione (GSH) levels, were measured at rest, after the Wingate test and during the 40 min of recovery. The recovery of exercise was associated with a significant increase (x2.7) in lipid radical production detected by ESR spectroscopy, as well as with changes in the erythrocyte GSH level (−13.6%) and SOD activity (−11.7%). The paradoxical decrease in plasma TBARS (−23.7%) which was correlated with the peak power developed during the Wingate test (r=−0.7), strongly suggests that such exercise stimulates the elimination of MDA. In conclusion, this study demonstrates that short-term supramaximal anaerobic exercise induces an oxidative stress and that the plasma TBARS level is not a suitable marker during this type of exercise.

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Acknowledgements

The authors thank Dominique Paul, Marie-Thérèse Gougeon, Catherine Stott Carmeni, Michel Monnier, and Marthe Godard for technical assistance and Dr Bennani-Dosse for statistical assistance. Experimental procedures were approved by the "Comité Consultatif des Personnes pour la Recherche Biomédicale (CCPPRB)" of Rennes.

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Authors and Affiliations

  1. Laboratoire de Physiologie et de Biomécanique de l'Exercice Musculaire, Campus la Harpe, UFRAPS Rennes 2, EA 1274, Avenue Charles Tillon, CS 24414, 35044 , Rennes Cedex, France

    C. Groussard, F. Rannou-Bekono, G. Machefer, S. Vincent & A. Gratas-Delamarche

  2. Laboratoire de Biologie Cellulaire et Végétale, Faculté de Pharmacie, Université de Rennes 1, 2 Avenue du Professeur Léon Bernard, EA 2231, 35043 , Rennes Cedex, France

    M. Chevanne, O. Sergent & J. Cillard

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  1. C. Groussard
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  2. F. Rannou-Bekono
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  3. G. Machefer
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  4. M. Chevanne
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  5. S. Vincent
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  6. O. Sergent
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  7. J. Cillard
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  8. A. Gratas-Delamarche
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Correspondence to C. Groussard.

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Groussard, C., Rannou-Bekono, F., Machefer, G. et al. Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise. Eur J Appl Physiol 89, 14–20 (2003). https://doi.org/10.1007/s00421-002-0767-1

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