Abstract
It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m·min–1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30% in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.
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Received: 1 August 1997 / Accepted: 13 November 1997
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Radák, Z., Nakamura, A., Nakamoto, H. et al. A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats. Pflügers Arch 435, 439–441 (1998). https://doi.org/10.1007/s004240050537
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DOI: https://doi.org/10.1007/s004240050537