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Comparison of acquired cholesteatoma between pediatric and adult patients

  • Otology
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Abstract

The quantification of angiogenesis and metalloproteinases may be useful in cholesteatoma behavior assessment as markers of its aggressiveness. The objective of this study is to compare markers CD31, MMP2 and MMP9 in pediatric and adult patients. This study is based on cross-sectional studies of pediatric (≤18 years old) and adult groups (≥19 years old). Samples of 120 cholesteatomas were fixed in 10% formol, prepared on five slides of each sample through habitual histological techniques, and number of blood vessels (CD31), marking with MMP2 and MMP9, number of matrix cells and thickness at perimatrix cell were observed. Data were analyzed through SPSS using Spearman and Mann–Whitney coefficients. Cholesteatomas were equally distributed: 60 in pediatric patients (11.77 ± 3.57 years); 60 in adult patients (38.29 ± 14.51 years). When correlating the number of blood vessels and metalloproteinases with perimatrix thickness, we obtained the following values: pediatric CD31, 7 (4–11); adult CD31, 4 (0–10) (P = 0.044); pediatric cytoplasmatic MMP2, 1 (0–3); adult cytoplasmatic MMP2, 0 (0–1) (P = 0.006); pediatric nuclear MMP2, 0 (0–1); adult nuclear MMP2, 0 (0–1) (P = 0.056); pediatric MMP9, 2 (0–4); adult MMP9, 0 (0–4) (P = 0.049). In conclusion, pediatric cholesteatomas present a more exacerbated inflammatory degree, produce more metalloproteinases, factors that, when combined, could characterize pediatric cholesteatomas as more aggressive than adult cholesteatomas.

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Acknowledgments

We would like to thank the Pathology and Ear, Nose and Throat Departments of the Hospital de Clínicas de Porto Alegre, for the permission to use their facilities and enable the development of this project. The Research and Post Graduation Group of the Hospital de Clínicas de Porto Alegre (GPPG-HCPA) and the Post Graduation Program in Medical Sciences: Pediatrics Department of the Universidade Federal do Rio Grande do Sul, for their technical support. The Fundo de Incentivo à Pesquisa e Eventos of the Hospital de Clínicas de Porto Alegre (FIPE-HCPA), for the research financing.

Conflict of interest statement

The authors did not have a financial relationship with the organization that sponsored the research.

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Authors and Affiliations

  1. Centro de Otite Média do Brasil, Serviço de Otorrinolaringologia, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Zona 19, sala 1922, Porto Alegre, CEP: 90003010, Brazil

    Cristina de Carvalho Dornelles

  2. Ophthalmology and Ear, Nose and Throat Department, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

    Cristina de Carvalho Dornelles & Sady Selaimen da Costa

  3. Surgery Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Sady Selaimen da Costa & Letícia Petersen Schmidt Rosito

  4. Pathology Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Luíse Meurer

  5. Ear, Nose and Throat Service, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, Brazil

    Cristina de Carvalho Dornelles & Letícia Petersen Schmidt Rosito

  6. SUS Jundiaí, São Paulo, Brazil

    Andrei Roberto da Silva

  7. Rua Ramiro Barcelos, 2350, sala 1922, São Paulo, Brazil

    Sabrina Lima Alves

Authors
  1. Cristina de Carvalho Dornelles
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  2. Sady Selaimen da Costa
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  3. Luíse Meurer
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  4. Letícia Petersen Schmidt Rosito
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  5. Andrei Roberto da Silva
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  6. Sabrina Lima Alves
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Corresponding author

Correspondence to Cristina de Carvalho Dornelles.

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de Carvalho Dornelles, C., da Costa, S.S., Meurer, L. et al. Comparison of acquired cholesteatoma between pediatric and adult patients. Eur Arch Otorhinolaryngol 266, 1553–1561 (2009). https://doi.org/10.1007/s00405-009-0957-0

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  • DOI: https://doi.org/10.1007/s00405-009-0957-0

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