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Promotion of cutaneous diabetic wound healing by subcutaneous administration of Wharton's jelly mesenchymal stem cells derived from umbilical cord

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Abstract

Wound healing is a major problem in diabetic patients, and current treatments have been confronted with limited success. The present study examined the benefit of Wharton's jelly mesenchymal stem cells (WJ-MSCs) derived from the human umbilical cord (UC) in wound healing in diabetic rats. Thirty days after inducing diabetes, a circular excision was created in the skin of rats, and the treatments were performed for 21 days. Two groups were studied, which included the Control group and WJ-MSCs group. The studied groups were sampled on the 7th, 14th, and 21st days after wounding. Histological ultrasound imaging of dermis and epidermis in the wound area for thickness and density measurement and skin elasticity were evaluated. Our results on post-wounding days 7, 14, and 21 showed that the wound closure, thickness, and density of new epidermis and dermis, as well as skin elasticity in the healed wound, were significantly higher in the WJ-MSCs group compared to the Control group. Subcutaneous administration of WJ-MSCs in diabetic wounds can effectively accelerate healing. Based on this, these cells can be used along with other treatment methods in the healing of different types of chronic wounds.

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Acknowledgements

This work was supported by Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran, Forensic medicine specialist, Research center of legal medicine organization of Iran, Tehran, Iran, and National Animal Modeling Network and In vivo Research, Council for Development of Stem Cell Sciences and Technologies, Vice-Presidency for Science and Technology, grant number 98/11626.

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Authors and Affiliations

  1. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Ali Nilforoushzadeh, Hamideh Afzali, Sona Zare, Shiva Alavi, Mona Mahmoudbeyk & Mohammad Amir Amirkhani

  2. Jordan Dermatology and Hair Transplantation Center, Tehran, Iran

    Mohammad Ali Nilforoushzadeh

  3. Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran

    Amir Raoofi & Omid Gholami

  4. Amol Faculty of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran

    Davood Nasiry

  5. Department of Medical Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

    Ebrahim Khodaverdi Darian

  6. Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran

    Ebrahim Khodaverdi Darian

  7. Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Auob Rustamzadeh

  8. Department of Biology, Hamedan Branch, Islamic Azad University, Hamedan, Iran

    Rahim Ahmadi

  9. Forensic Medicine Specialist, Research Center of Legal Medicine Organization of Iran, Tehran, Iran

    Alimohammad Alimohammadi

  10. Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Zahra Razzaghi

  11. Department of Pathology, Razi Skin Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Zahra Safaie Naraghi

  12. Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, Campinas, Caixa Postal: 6121.CEP: 13083-862, São Paulo, Brazil

    Amin Mousavi Khaneghah

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  1. Mohammad Ali Nilforoushzadeh
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Correspondence to Sona Zare.

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Nilforoushzadeh, M.A., Raoofi, A., Afzali, H. et al. Promotion of cutaneous diabetic wound healing by subcutaneous administration of Wharton's jelly mesenchymal stem cells derived from umbilical cord. Arch Dermatol Res 315, 147–159 (2023). https://doi.org/10.1007/s00403-022-02326-2

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