Studies on broccoli (Brassica oleracea var. italica) indicate beneficial effects against a range of chronic diseases, commonly attributed to their bioactive phytochemicals. Sulforaphane, the bioactive form of glucoraphanin, is formed by the action of the indigenous enzyme myrosinase. This study explored the role that digestion and cooking practices play in bioactivity and bioavailability, especially the rarely considered dose delivered to the colon.
The antimicrobial activity of sulforaphane extracts from raw, cooked broccoli and cooked broccoli plus mustard seeds (as a source myrosinase) was assessed. The persistence of broccoli phytochemicals in the upper gastrointestinal tract was analysed in the ileal fluid of 11 ileostomates fed, in a cross-over design, broccoli soup prepared with and without mustard seeds.
The raw broccoli had no antimicrobial activity, except against Bacillus cereus, but cooked broccoli (with and without mustard seeds) showed considerable antimicrobial activity against various tested pathogens. The recovery of sulforaphane in ileal fluids post soup consumption was < 1% but the addition of mustard seeds increased colon-available sulforaphane sixfold. However, when sulforaphane was extracted from the ileal fluid with the highest sulforaphane content and tested against Escherichia coli K12, no inhibitory effects were observed. Analysis of glucosinolates composition in ileal fluids revealed noticeable inter-individual differences, with six “responding” participants showing increases in glucosinolates after broccoli soup consumption.
Sulforaphane-rich broccoli extracts caused potent antimicrobial effects in vitro, and the consumption of sulforaphane-enriched broccoli soup may inhibit bacterial growth in the stomach and upper small intestine, but not in the terminal ileum or the colon.
Broccoli (Brassica oleracea var. italica) is a member of the Brassicaceae family of plants. Epidemiological studies, dietary trials, animal and in vitro investigations have revealed a range of potential beneficial effects of broccoli on a range of chronic diseases such as different types of cancers [1,2,3,4] and cardiovascular diseases [5,6,7]. The beneficial effects are commonly attributed to bioactive phytochemicals present that includes hydroxycinnamic acids, flavonoids (e.g., quercetin, kaempferol, myricetin), carotenoids (lutein) and glucosinolates (e.g., glucoraphanin) [8,9,10].
Glucosinolates are of great interest; in broccoli glucoraphanin is present in the highest quantity, comprising about 80% of the total, with smaller amounts of glucobrassicin, 4-methoxyglucobrassicin and 1-methoxyglucobrassicin also being found . The biologically active forms of the glucosinolates are isothiocyanates which are generated by the action of the enzyme myrosinase that is released when the plant tissue is chopped or chewed . In the case of glucoraphanin, the isothiocyanate formed is sulforaphane, less bioactive nitriles can also be formed by the activity of epithiospecifier protein (ESP) . Sulforaphane has been shown in vitro to exert bioactivity [14,15,16,17,18] and to exert antibacterial effects against a range of food-borne pathogens and enteropathogenic microbes including Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Helicobacter pylori [9, 19,20,21].
The effect of cooking Brassica vegetables on the absorption of isothiocyanates has been well studied . It was reported that only 3.4% of sulforaphane was recovered in blood and urine after consuming microwaved broccoli due to myrosinase inactivation . Similarly, isothiocyanates levels were three-fold higher in urine after consumption of raw broccoli compared with steamed broccoli (32.3% vs 10.2%) . Fahey et al.  also demonstrated that active myrosinase was critical to the bioavailability of glucoraphanin in broccoli extracts as sulforaphane was 3–4 times more bioavailable in extracts with an active myrosinase enzyme than in extracts without. Similarly, Ghawi et al.  reported that the addition of mustard seeds, which contain a more resilient isoform of myrosinase, to heat-processed broccoli increased the formation of sulforaphane by 3–5fold.
In terms of bioavailability, Johnson  reported that isothiocyanates were absorbed through the small bowel and colon, where their metabolites were detected 2–3 h post consumption, with Petri and colleagues in an elegant study showing high levels of absorption for sulforaphane and quercetin 3,4'-diglucoside from an onion and broccoli extract by enterocytes using a perfused jejunal segment and determined that a proportion was effluxed back into the lumen as sulforaphane-glutathione and quercetin-3'-glucuronide . An often-neglected aspect, however, is the role that digestion and cooking practices play in bioactivity and bioavailability, especially in relation to amounts of phytochemicals delivered to the colon.
In the present study, we have used ‘Beneforté broccoli’, a new variety that contains enhanced levels of glucoraphanin , which previous studies have demonstrated a threefold greater increase in the concentration of plasma sulforaphane compared to standard broccoli . The aim of this study was two-fold: firstly to establish the antimicrobial activity of sulforaphane extracts from raw, cooked broccoli and cooked broccoli with a mustard seed (to provide myrosinase activity), and secondly to determine the persistence of broccoli phytochemicals and their metabolites in the upper gastrointestinal tract by analyzing the ileal output from ileostomates fed with a broccoli soup prepared with or without mustard seeds.
Materials and methods
Broccoli powder preparation and thermal processing
Beneforté broccoli was supplied by Staples Vegetables Ltd. (Boston, Lincolnshire, UK); mustard seeds (MS) were sourced from a local supermarket. Broccoli heads were cut (around 4–5 cm from top) and combined thoroughly. For the cooking experiments, 50 g portions were vacuum-packed in flexible polyethylene bags with dimensions of 24 × 24 cm, to stop glucoraphanin leaching into the processing water. Using a thermostatic water bath, broccoli was cooked at 100 °C for 12 min. Mustard seeds were ground and added as a powder. All samples of raw and cooked broccoli were frozen in dry ice before storage at −80 °C; following this, samples were lyophilized (VirTis SP Scientific, UK), ground using a coffee grinder and sieved (30 mesh). Samples were finally stored at −20 °C until further analysis. The solids content of fresh broccoli was 11.9% (w/w).
Preparation of broccoli soup for the intervention study
The broccoli powder was prepared in the pilot plant facilities at the Department of Food and Nutritional Sciences at the University of Reading. All other ingredients were purchased from local stores at reading, uk. soup preparation was conducted at the pilot plant facilities. Good manufacturing practices (GMP) and hazard analysis critical control point (HACCP) were implemented throughout the preparation process to ensure the safety of the products. Ingredients to prepare the soup for the intervention study were shipped to Ulster University. Soup was locally prepared fresh prior to consumption. In brief, ingredients (Supplementary material 1), except broccoli powder and mustard seed powder, were dissolved in 50 ml cold water, and then added to 11 g broccoli powder in 150 ml boiling water. The resulting soup was then left to boil for 3 additional min to inactivate ESP . The soup was left to cool down to 60 °C, and then mustard seed powder was added. The soup was allowed to stand for 10 min before it was served. Thirty-six untrained panellists were recruited to participate in a consumer evaluation test to determine the soup palatability at different broccoli powder concentrations (Supplementary material 2).
Extraction and analysis of glucoraphanin from broccoli powder
Extraction of glucoraphanin from the broccoli samples was conducted as described previously by Oerlemans et al.  with minor modifications. Glucotropaeolin (0.25 ml of 1 mg/ml) (Santa Cruz Biotechnology, Heidelberg, Germany) was used as an internal standard. Freeze-dried samples (0.2 g) were extracted three times with 70% methanol at 70 °C for 10 min; these conditions were selected to inactivate myrosinase and avoid hydrolysis of the glucosinolates. Samples were centrifuged (3500g, 5 min), supernatants were combined and filtered (0.45 µm syringe filter) and finally topped up to 10 ml using 70% methanol. The extract was then purified using a mini column (HiTrap DEAE FF anion exchange column, 1 ml) (GE Healthcare, Chalfont, UK). Briefly, the column was washed five times with deionised water (1 ml each), and then 1 ml of the crude extract was passed through the column. The column was washed from unbound compounds with deionized water (2 × 1 ml) and sodium acetate buffer (2 × 1 ml; 20 mM, pH 5.0). Sulfatase enzyme (75 µl, 35 mg/ml) (Type H-1 from Helix pomatia, Sigma, Poole, UK) was added, and the column was incubated overnight at room temperature. The desulfo-glucosinolates were then eluted from the column using deionised water (4 × 1 ml). The eluate was dried using a rotary evaporator (70 °C) and re-dissolved in 0.5 ml of deionised water. The purified extract was filtered (0.45 µm syringe filter) and injected onto the HPLC. The desulfo-glucosinolates analysis was conducted with an HPLC–UV system (Agilent 1200, Manchester, UK) using an ACE 5 C18 reverse-phase column (150 × 4.6 mm) (Hichrom) with a flow rate of 1 ml/ min; absorbance measurement was conducted at a wavelength of 229 nm. The mobile phase was (A) water and (B) 20% acetonitrile. The following gradient was used: 100% (A) and 0% (B) for 1 min, B increased up to 100% over 20 min, returning to 100% (A) over 5 min and holding at 100% (A) for 4 min. The column temperature was set at 30 °C and the injection volume was 10 µl. Glucoraphanin quantification was carried out using desulfo-glucoraphanin as an external standard. All glucosinolate standards were sourced from Santa Cruz Biotechnology, Heidelberg, Germany. Organic solvents (HPLC-grade) were from Fisher Scientific (Loughborough, UK).
Extraction and GC–MS analysis of sulforaphane and sulforaphane nitrile from broccoli powder
Extraction of sulforaphane and its nitrile analogue (sulforaphane nitrile) from broccoli samples was conducted as described by Ghawi et al.  with some modifications. 0.5 g of lyophilised broccoli powder was placed in a tube with 10 ml deionized water and incubated for 1 h at 30 °C. These conditions were selected to ensure complete hydrolysis of glucosinolates by myrosinase. Samples were centrifuged (5000g, 5 min) and the supernatant was collected; this step was repeated twice, and the supernatants were combined. To extract isothiocyanates and the nitrile analogues from the supernatant, 10 ml of dichloromethane were added to 10 ml of supernatant in a glass tube, vortexed for 1 min and centrifuged (5000g for 5 min); then the organic phase was collected. The extraction process was repeated two more times and the extracts were combined. About 3 g of unhydrated sodium sulphate were added to the extract to remove any excess water. The extract was then dried using a rotary evaporator (30 °C) and the eluate was re-dissolved in 0.7 ml dichloromethane and filtered using 0.22 µm filter.
The extracts were analyzed using GC–MS for sulforaphane and sulforaphane nitrile. GC–MS was performed using an Agilent 7850/5975 GC–MS system (Agilent, UK). The samples (1 µl) were injected onto a ZB-AAA capillary column (Phenomenex, USA) (0.25 µm film thickness, 15 m × 0.25 mm i.d.). The injection temperature was 250 °C and the split mode 1:20. The oven temperature was programmed from 110 to 320 °C at a rate of 30 °C/min. The flow rate of the helium carrier gas was 1.1 ml/min and the transfer line temperature held at 320 °C. Mass spectra were obtained by electron ionisation at 70 eV, and the mass scan was from 35 to 500 amu. Quantification of sulforaphane and sulforaphane nitrile was based on sulforaphane external standard.
Extraction and HPLC analysis of sulforaphane from the soup and ileal fluids
Sulforaphane was extracted from broccoli soup based on the methodology described by Ghawi et al. . One ml of the soup was centrifuged at 5000g, and the supernatant was collected; the pellet was extracted twice using 10 ml distilled water, and the supernatants were combined. Sulforaphane was extracted from the supernatant using 10 ml of dichloromethane for 1 min; the extraction step was carried out twice. The organic layers were collected (about 15 ml) and ~ 4 g sodium phosphate was added to remove any remaining water. Dichloromethane was evaporated using a rotary evaporator, and the pellet was dissolved in 0.6 ml of acetonitrile and filtered using a 0.45 µm filter.
Sulforaphane was extracted from ileal fluid samples as follows: 15 ml of the ileal fluid was centrifuged (14,500g, 10 min, 10 °C) and the supernatant was collected. The pellet was re-extracted with 10 ml water, and the supernatants were then combined. Sulforaphane was extracted from the supernatant using dichloromethane and treated as described above.
Ten microlitres of the filtrate were injected in an HPLC–UV system (Agilent 1200, Manchester, UK) using an ACE 5 C18 reverse-phase column (150 × 4.6 mm) (Hichrom) with a flow rate of 1 ml/min. The mobile phase was (A) water and (B) 100% acetonitrile. The following gradient was used: 100% (A) and 0% (B) for 1 min, B increased up to 100% over 20 min, returning to 100% (A) over 5 min and holding at 100% (A) for 4 min. The column temperature was set at 30 °C, whereas the absorbance was measured at 254 nm. Serial dilutions of sulforaphane standards (10–1600 µg/ml) were used to construct the standard curve. Organic solvents (HPLC-grade) were from Fisher Scientific (Loughborough, UK).
For comparison of sulforaphane concentrations by both GC and LC methods, the mean of each data set was used for statistical analysis. The Shapiro–Wilk test was used to test for normality and the Mann Whitney U test was used to compare groups. Significance was accepted at p < 0.05 unless otherwise specified. Analysis was carried out using SPSS (version 25 for Windows).
Assessment of antimicrobial activity of sulforaphane extracts from broccoli powder
The bacteria used to test the antimicrobial activity of broccoli sulforaphane extracts included strains of the genera Salmonella, Escherichia, Staphylococcus, Listeria and Bacillus. Information on each strain and its origin is provided in Supplementary material 3. To understand the impact of broccoli sulforaphane extracts on food or in the gut, all bacterial strains with the exception of E. coli K12 used in these experiments are gram-positive and -negative foodborne pathogens. E. coli K12 is the most commonly used strain in microbiological research  and its use facilitated further genetic research we conducted on the antibacterial mode of action of broccoli sulforaphane extracts. We also included the pathogenic E. coli O157:H7-VT strain which although it is unable to produce verotoxin, it shares all other features with pathogenic strains. To get an insight on the antimicrobial mechanism of broccoli sulforaphane extracts we also included two S. Typhimurium strains, one with an intact and the other with defective RpoS. RpoS is the main stress gene regulator in Salmonella enterica and the majority of gram-negative bacteria . We also included a pathogenic L. monocytogenes strain originally isolated from the human disease  with its isogenic mutant in glutamate decarboxylase gadD2 that plays a role in antimicrobial resistance . All bacterial strains were stored in 30% glycerol at −80 °C. The cells were cultured on nutrient agar (Oxoid) for up to 2 days, then three single colonies were obtained and transferred into 2 ml of phosphate buffer saline (PBS) solution. The turbidity of the suspensions was adjusted to match approximately a 0.5 McFarland standard (Fisher Scientific). The antimicrobial activity was tested using the disc diffusion method as described by Aires et al. . In brief, from a bacterial suspension, a sterile cotton swab was spread onto Petri dishes (90 mm diameter) containing 20 ml of Mueller–Hinton Agar (Oxoid). Sterile filter paper discs (6 mm diameter; Oxoid) were impregnated with 100 µl of extracted sulforaphane samples and then placed on the agar plate; the plates were incubated in an inverted position overnight at 37 °C. The equivalent volume of solvent (acetonitrile) without sulforaphane extract was used as a negative control. Antibiotics, namely chloramphenicol (30 µg/ml), ampicillin (10 µg/ml), gentamicin (10 µg/ml) and tetracycline (30 µg/ml) (Oxoid) were used as positive controls. After overnight incubation, the diameter of the inhibitory zones around the disc was measured and recorded. All tests were performed in triplicate and the antibacterial activity was expressed as the mean of the diameters (in mm) of the inhibition zones. T test was used to compare the means of antibacterial activity, means were considered statistically different if p < 0.05 (Excel, Microsoft Office 365 ProPlus).
To determine the lowest concentration of sulforaphane needed to exert an antimicrobial effect, the minimum inhibition concentration (MIC) assay was conducted in a 96-well plate, as described by Wiegand et al.  using E. coli K12 as the indicator microorganism. Briefly, the 96 well plate was loaded with 150 µl of nutrient broth inoculated with 107 CFU/ml of an overnight E. coli K12 culture (cell concentration of overnight culture ~ 109 CFU/ml). Immediately after the addition of the culture, 150 µl of 2 mg/ml sulforaphane (LKT Laboratories, USA) were added in the first well, and then serial dilutions were made to achieve a range 1–0.03 mg/ml of sulforaphane. The plate was incubated at 37 °C in a micro-plate reader for 24 h and the optical density measured at 620 nm and plotted over time. The experiment was performed in triplicate for each dilution.
Dietary intervention study with ileostomy patients
The use of ileostomy patients combined with metabolomics can provide insight into the processes of biotransformation and absorption in the upper gastrointestinal tract that impact the phytochemicals in the ingested plant material, in the case of broccoli glucoraphanin and sulforaphane, potentially influencing bioactivity and health benefits [36, 37]. The ileostomy feeding study, an acute randomised, single-blind, placebo-controlled crossover study with 1-week washout, used a soup prepared (described earlier) from Beneforté broccoli, a hybrid of Brassica oleracea and Brassica villosa , supplied by Staples Vegetables Ltd (Boston, Lincolnshire, UK) and mustard seeds were sourced from a local supermarket (ASDA, Reading, UK). Soup was chosen as the intervention food product to ensure that the participants received a homogenous and uniform food providing a consistent quantity of broccoli phytochemicals delivered in the form of a powdered broccoli-based soup. Thereby minimising the impact of any interplant composition variation and ensuring that effects observed biologically were a consequence of inter-person variation in ADME alone. As no studies existed on broccoli consumption and ileostomists, a 4-h post-consumption sampling point selected to ensure capture of the appearance of phytochemicals from the intervention food. This choice was consistent with ileostomy studies that used a liquid-based vehicle to deliver phytochemicals and showed substantive quantities to be evident in ileal fluid after 4 h [38, 39]. While a one week washout period for the study was considered appropriate as it ensured that broccoli phytochemicals would have passed through the gastrointestinal tract after consumption of a single bolus meal (soup, 200 ml).
Ethical approval (14/SC/1326) for the study was received from the South Central-Hampshire Research Ethics Committee and Ulster University. All participants gave written informed consent and the study was conducted in accordance with the Helsinki Declaration. Participants were recruited between Jan 2015 and Feb 2015 from clinics at Altnagelvin Hospital. The intervention study ran between March 2015 and April 2015. The study was registered at clinicaltrials.gov as NCT04113928. The study was conducted in 11 ileostomists (7 males, 4 females) age range 32–63 years, who had undergone terminal ileostomies and were at least 1.5 years post-operative prior to the study and were non-smokers.
After obtaining consent, participants were randomly assigned, in blocks of four using a random-number generator (www.randomization.com), to either the intervention or the control. In total, 12 participants were randomised to 2 groups. One participant subsequently withdrew from the study prior to sampling. Participants were asked to follow a restriction diet, avoiding dark green leafy vegetable and mustard-like vegetables especially broccoli, cabbage, Brussels sprouts, watercress, rocket, spinach, onions, spring onions, radish, horseradish for 48 h before each clinic visit. Following an overnight fast, the participants provided an ileal fluid sample (T 0 h) then consumed 200 ml of freshly prepared broccoli soup (described above) with/without the addition of mustard seed, within 15 min, a second ileal fluid sample was collected 4 h post consumption (T 4 h). The ileal fluid samples were collected and processed as described in McDougall et al. . Weights and pH of the ileal fluid were recorded, before dilution with ice-cold distilled water as required, dependent on viscosity, before being homogenised in a chilled Waring blender for 30 s, and storage of aliquots at −80 °C in preparation for subsequent analysis.
Targeted analysis of glucosinolates in ileal fluids
Ileal fluids were extracted using the procedure outlined previously . Briefly, frozen ileal fluid samples were thawed and vortex-mixed, and duplicate 2.0 ± 0.1 g samples were weighed into 15 ml centrifuge tubes. These were extracted using 3 ml of ultrapure water containing aqueous 0.1% formic acid and 20 mM diethyl dithiocarbamate (DDC). The tubes were vortex-mixed for 3 × 30 s, then sonicated in a water bath for 1 min. All procedures were carried out at 5 °C. After centrifugation (2500g, 10 min, 5 °C), the supernatants were transferred to new tubes. The pellets were extracted twice using 3 ml of 0.1% formic acid in methanol containing 20 mM DDC, and the supernatants combined and vortex-mixed. A subsample of 4 mL was removed and dried in a Speed-Vac. The dried samples were resuspended in 0.5 mL of 10% (v/v) acetonitrile containing 500 µM morin as an internal standard then transferred into filter vials (Thomson, 0.45 μM PTFE filter vial: Bioprocess Engineering Services Ltd., Ashford, Kent, UK). The samples were analysed by liquid chromatography-mass spectrometry (LC–MS) using an Agilent 1260 Infinity HPLC 6230 time of flight (TOF) mass spectrometry system operated under MassHunter (Agilent, Ltd. UK) software. The TOF/MS system was tuned and calibrated according to the manufacturers recommended procedures for use across 80–2000 m/z in negative mode. Sub 3 ppm mass accuracies were achieved by applying the recommended reference mass locking solution to a secondary ESI probe via a secondary HPLC pump at a flow rate of 2.5 mL/min with 1:100 splitter (25 μL/min to source) using the m/z values at 112.985587 and 1033.988109 in the negative mode as standard reference locks. The method for quantification of Gls is given in the Supplementary material 4.
Data handling and statistical analysis for non-targeted analysis
The LC–MS raw data were converted into MZML centroid format using the Proteowizard MSConvert software package (https://proteowizard.sourceforge.net/). The data was then deconvoluted using the XCMS online package (https://xcmsonline.scripps.edu/) producing a Microsoft Excel-based XY matrix of the paired RT and m/z of each feature against the peak intensity for each sample. The dataset was next subjected to automated peak annotation workflows within PutMedID [41, 42] which grouped m/z features likely associated with the same compound (i.e., a ‘peak group’). Accurate mass differences between m/z within each peak group were next calculated to allow the annotation of the parent m/z, isotope and adduct ions, as well as common in-source fragments. The neutral accurate mass was then matched to libraries of possible metabolites (Plant Metabolic Network PlantCyc database (https://www.plantcyc.org/ and the Manchester Metabolomics Database (MMD: https://dbkgroup.org/MMD/). After annotation, non-grouped low intensity features, redundant isotope, adduct and fragment features were removed from the dataset to reduce data complexity. Principal components analysis and optimized partial least squares-discriminant analysis was applied to the XCMS data to identify components that increased in the after broccoli ileal fluids. Full details are given in Supplementary material 5.
Antimicrobial activity of sulforaphane extracted from ileostomy fluids against Escherichia coli K12
To assess the antimicrobial activity, sulforaphane was extracted from ileostomy fluid in a similar way to that from soup, with the exception that the final pellet was dissolved in 0.6 ml nutrient broth (Oxoid) instead of acetonitrile. The concentration of sulforaphane in nutrient broth was 3.14 µM. A 96-well plate titration method was used to assess the inhibitory effect of extracted sulforaphane against E. coli K12 cells. The sulforaphane containing nutrient broth was filtered through a 0.20 µm filter, and 200 µl were dropped into each well. E. coli K12 grown overnight in nutrient broth at 37 °C was used as inoculum. Each well was inoculated with an impregnate needle, and the plates were incubated at 37 °C for 24 h; the absorbance was measured at 620 nm using a plate reader (Sunrise™, Tecan, Austria). The obtained growth curves were compared with that of the control (no sulforaphane addition) in terms of their lag phase, growth curve pattern and final optical density to evaluate the effect of antimicrobial effect of sulforaphane.
Quantification of glucoraphanin, sulforaphane and sulforaphane nitrile in broccoli samples
The glucoraphanin content in raw broccoli was 10.68 µmol/g broccoli powder and cooking did not significantly reduce content (9.97 µmol/g) (Fig. 1). Sulforaphane was low in raw broccoli (0.57 µmol/g DW) with significantly higher levels of sulforaphane nitrile (9.9 µmol/g DW). Cooking of broccoli did not increase the concentration of sulforaphane (0.58 µmol/g DW) compared to raw (Fig. 1), probably as cooking inactivated the endogenous myrosinase activity. However, the addition of mustard seed powder (2%) to cooked broccoli significantly (P < 0.05) increased sulforaphane formation from 0.58 to 10.90 µmol/g DW. Although sulforaphane nitrile level increased after the addition of mustard seeds (0.05 vs 0.69 μmol/g DW), this increase is low compared to the level formed in broccoli before cooking (0.69 vs 9.9 µmol/g DW).
Sulforaphane content in broccoli soups
Following the initial studies, a broccoli soup was prepared which contained 26.5 µmol of sulforaphane per 200 ml portion (0.13 µmol/ml soup). The addition of 2% mustard seed powder at the cooling stage of the soup preparation process (~ 60 °C) increased the level of sulforaphane by nearly fourfold in the soup, 102 µmol per 200 ml portion (0.51 µmol/ml soup).
Antimicrobial activities of broccoli sulforaphane extracts
The raw broccoli extracts showed no antimicrobial activity (Table 1), except against B. cereus. In contrast, the extracts from cooked broccoli (with and without mustard seeds) showed considerable antimicrobial activity against the tested strains. Furthermore, the extracts from cooked broccoli with added mustard seed exhibited significantly higher activity (T test; p < 0.05) than the cooked broccoli without MS. This increased activity could be interpreted as being due to the increased levels of isothiocyanate, mainly sulforaphane (Fig. 1), formed from glucoraphanin (~ 81% of total glucosinolate content in broccoli ), presumably due to the activity of myrosinase from the MS. Other compounds such as allyl isothiocyanate originating from MS may contribute to the antimicrobial activity, however, this contribution is likely to be minor as MS was added in very small quantity to broccoli (2%). The greatest zone of inhibition was reported for B. cereus (13.7–46.7 mm). Lower antimicrobial activities were generally observed for the gram-positive bacteria such as S. aureus and L. monocytogenes strains compared to the gram-negative ones such as S. enterica, and E. coli.
Interestingly, all cooked broccoli extracts demonstrated a strong inhibitory effect against both the antibiotic-resistant S. Typhimurium str.10 and str.30 (inhibition zones ranging from 10 to 25 mm; Table 1). With E. coli and S. Typhimurium strains, the cooked broccoli with MS extracts were as effective as gentamycin. The cooked broccoli extracts also demonstrated an inhibitory effect on L. monocytogenes (inhibition zones ranging from 10 to 18 mm). The minimum inhibitory concentration (MIC, the concentration at which cell growth was completely inhibited) for pure sulforaphane against E. coli K12 was established at 1 mg/ml (5.65 mM), (Supplementary material 6), with concentrations as low as 0.03 mg/ml exerting some inhibition of bacterial growth.
Ileostomy feeding study with broccoli soup
Eleven ileostomates (males n = 7, females n = 4) were enrolled on this acute randomised, single-blind, placebo-controlled two-way crossover trial. The intervention was conducted as per the protocol and with the consent of participants, all 11 subjects completed the study and there were no adverse events associated with the consumption of broccoli soup (Fig. 2). The study population had a mean group age of 53.3. ± 9.2 years. The average weight of ileal fluid day 1 (0 h) 233 g ± 84, pH 5.9 ± 0.7, day 2 214 g ± 84, pH 5.9 ± 0.6.
The group mean content of sulforaphane in the ileal fluids from subjects given broccoli without mustard seeds was 0.17 µmol, with considerable inter-individual variation (Table 2). This represents a recovery of < 1% of the applied sulforaphane in the original soup. The addition of MS to the broccoli soup significantly increased the group mean amount of sulforaphane in the ileal fluids to 1.05 µmol (a six-fold increase), once again with a substantial inter-individual variation. This is a significant increase in colonic availability of sulforaphane.
Glucosinolates (GIs) content and composition were quantified in ileal fluids before and after broccoli soup intake using targeted LC–MS TOF analysis against individual glucosinolate standards. There were considerable inter-individual differences in response; only six of the eleven subjects (SUBJs 1, 7, 8, 10, 11 and 12) showed clear increases in GIs after soup intake (Fig. 3) and there was considerable variation in the composition and levels of GIs between the responders. The five “non-responding” subjects either had high levels of GIs in pre-intervention samples (SUBJs 2, 5 and 6) or showed very low levels of GIs in any samples (SUBJs 4 and 9). The GIs composition in the ileal fluids from the responders had glucoraphanin as the dominant component but there was considerable variation in the composition of other GIs between the responding subjects. In SUBJs 10 and 12, Gls content was more pronounced in the broccoli soup without mustard seeds. This is expected as myrosinase in broccoli was inactivated during soup preparation. However, the same pattern does not hold for the other responders.
In addition, a non-targeted approach was applied to identify components that also increased in ileal fluids following broccoli soup intake. The MS data were deconvoluted using XCMS software and multivariate statistical analyses (Principal Component and OPLS-DA analysis) were employed to identify components that increased after soup intake (Supplementary material 7, Figs. 1, 2, 3, 4). As expected, the non-targeted analysis of the responders also identified the presence of the same GIs in the ileal fluids (Supplementary material 8), confirming the targeted analysis. Allyl isothiocyanate (m/z 98; predicted formula C4H5NS) was also detected, which could arise from the mustard seeds as a breakdown product of sinigrin .
However, other components were putatively identified which most probably arise from the broccoli consumed. The phenolic compound, feruloyl-sinapoyl-gentiobiose (m/z 723; predicted formula C33H40O18), is characteristic of broccoli florets (https://foodb.ca/compounds/FDB002177) but can be found in other Brassicas. Kaempferol sophoroside (m/z 609, C27H30O16) was also identified and this is known to be a major phenolic component of broccoli (https://phenol-explorer.eu/contents/food/263). Feruloyl putrescine was also identified and this has been confirmed in other studies on broccoli . A component with m/z 163 (predicted formula C9H8O3) was also identified which could be phenylpyruvate. The absence of other more abundant broccoli phenolics (as detailed in https://phenol-explorer.eu/contents/food/263) probably reflects their relative stability in the gut.
Antimicrobial effect of sulforaphane extracted from ileostomy fluid against E. coli K12
Following analysis of the ileal samples (Fig. 2), subject S04 was identified as having the highest amounts of sulforaphane at 3.14 µmol (equivalent to 41 µM) and was selected as a candidate to assess inhibitory effect against E. coli K12. It was not possible to carry out anti-microbial assessment using the ileal fluid per se, given the inherent presence of small intestinal microbiota, consequently sulforaphane was extracted from the ileal fluid, dried, and re-constituted to the original ileal fluid concentration (41 µM) in nutrient broth to evaluate its antimicrobial activity against E. coli K12. The results indicated no difference in the growth of E. coli in the presence of sulforaphane compared to the control (data not shown). The MIC of pure sulforaphane against E. coli K12 was 5.65 mM (1 mg/ml) but 171 µM (0.03 mg/ml) exerted some inhibition of bacterial cell growth.
Studies on broccoli (Brassica oleracea var. italica) indicate beneficial effects against a range of chronic diseases, commonly attributed to their bioactive phytochemicals. Sulforaphane, the bioactive form of glucoraphanin, is formed by the action of the enzyme myrosinase. This study explored the role that digestion and cooking practices play in bioactivity and bioavailability, especially the rarely considered dose delivered to the colon.
The processing of the broccoli from raw to the cooked forms with and without the added enzyme myrosinase, in the form of mustard seeds, altered the phytochemical composition of the various broccoli powders (Fig. 1). Cooking broccoli did not significantly reduce the glucoraphanin content and indeed only limited glucosinolate thermal degradation has been noted at processing temperatures lower than 110 °C [30, 45]. Consequently, the cooking process likely denatured broccoli’s endogenous thermo-labile myrosinase preventing hydrolysis of the glucosinolates. Sulforaphane was low in raw broccoli but higher levels of sulforaphane nitrile were present, which agrees with previous literature [46,47,48]. The formation of sulforaphane nitrile rather than sulforaphane in raw broccoli is due to the action of epithiospecifier protein (ESP), a non-catalytic cofactor of myrosinase that promotes the formation of epithionitriles from alkenyl glucosinolates. The formation of sulforaphane nitrile was substantially reduced after cooking which indicates that ESP, the highly temperature-sensitive protein , was inactivated during cooking. The addition of mustard seeds powder (2%) to cooked broccoli soup significantly increased sulforaphane formation (fourfold) while sulforaphane nitrile was detected at low levels. Therefore, the addition of low concentrations of mustard seeds powder, which contains a thermally resistant myrosinase [45, 49], to cooked broccoli has the potential to intensify sulforaphane formation.
The in vitro antimicrobial activities of broccoli sulforaphane extracts were in line with the results from previous studies which have shown that pure sulforaphane has a broad antimicrobial spectrum effect against both gram-negative and gram-positive bacteria [19, 20, 50,51,52,53]. However, the present study showed that gram-negative bacteria, such as E. coli and S. enterica were more susceptible to sulforaphane than gram-positive bacteria, such as L. monocytogenes and S. aureus, with the exception of the gram-positive B. cereus. In this study, we used two highly similar S. Typhimurium DT104 strains (strains 10 and 30). Strain 30 possesses a fully functional RpoS, whilst strain 10 possesses a defective RpoS due to an amber non-sense codon . RpoS is the main stress gene regulator in S. enterica and other gram-negative bacteria involved in resistance to a variety of stresses . Interestingly, both strains were equally susceptible to broccoli extracts suggesting that RpoS and its regulon that comprises hundreds of stress genes may not play a role in resistance against broccoli extracts or sulforaphane. We also included a mutant in the glutamate decarboxylase gene gadD2 in L. monocytogenes which plays a role in antimicrobial resistance, did not show a different behaviour comparing to the wild type E. coli K12 suggesting that also this gene does not play a role in resistance to broccoli extracts or sulforaphane.
Bacteria, such as E. coli O157: H7, S. Typhimurium DT104 and L. monocytogenes, which were tested in this study are associated with gastrointestinal infections and food poisoning and can cause considerable health problems to humans. For instance, E. coli O157:H7 can cause acute haemorrhagic diarrhoea . Enterotoxigenic E. coli can colonize the upper bowel and cause watery diarrhoea which in some cases can lead to death , and S. Typhimurium DT104 can also cause diarrhoea, fever, headache, nausea, abdominal pain and vomiting. L. monocytogenes is another important potentially pathogenic bacterium which can cause listeriosis through the colonisation of the gastrointestinal tract, which is one of the deadliest foodborne infections in the Developed World . While the antimicrobial activity of the broccoli extracts was comparable to ampicillin, chloramphenicol, tetracycline and gentamycin (Table 1). It should be stated that this comparison is not based on weight since antibiotics are normally used in low quantities. However, this comparison is valid since the concentrations of antibiotics and broccoli used in these experiments are comparable to the levels that would be found in the intestinal tract following ingestion of broccoli and that in an infection site following administration of these antibiotics. Overall, the results obtained from this study, suggest that cooked broccoli extracts might be promising natural agents for controlling human pathogens during the gastrointestinal digestion of foods, with the addition of myrosinase potentiating the antimicrobial activity possibly due to the enhanced level of sulforaphane. Considered from the in vivo perspective, following consumption of the broccoli soup(s) the ileal recovery of sulforaphane was < 1% which indicates early absorption in the small intestine consistent with observations by Petri et al.  who reported ~ 74% absorption of sulforaphane in a perfused jejunum section and highlighted that a proportion was conjugated to sulforaphane-glutathione and excreted back into the lumen and, therefore, low colonic-availability. However, the addition of MS to the broccoli soup significantly increased the amount of sulforaphane available to the colon by sixfold, although there were considerable inter-individual differences in responses evident. Similarly, a study in healthy participants reported that consumption of broccoli (200 g) with brown mustard seeds increased urinary excretion of the sulforaphane metabolite sulforaphane N-acetyl-l-cysteine fourfold . Sulforaphane extracted from the ileal fluid of subject S04 (which had the highest sulforaphane content) did not show antimicrobial activity against E. coli K12 presumably as the sulforaphane concentration was below the observed MIC (Supplementary material 6).
The targeted and non-targeted LC–MS approaches adopted in the study identified the presence of the expected GIs in the ileal fluids. Other compounds were also identified in the non-targeted approach including B-vitamins such as pantothenamide (m/z 217, C9H18N2O4) and metabolites of biotin (vitamin B7), norbiotin (m/z 229, C9H14N2O3S), bisnorbiotin (m/z 215, C8H12N2O3S) and biotin sulfoxide (m/z 259, C10H16N2O4S). Broccoli has a low content of biotin so these metabolites probably arise from the cheese and milk powder in the soup mix (Table in Supplementary material 1). Pantothenamide is a food additive used as a precursor for pantothenic acid (vitamin B5) and probably also arises from these other soup ingredients. There are many putative peptides in the post-soup ileal fluids. Whilst one of these (e.g., Leu-Leu; leucyl-leucine; m/z 243, C12H24N2O3) has been identified in broccoli previously , the others could arise from the digestion of proteins in the cheese and/or sauce powder used in the formulation of the soups. It is intriguing that these components from the more prosaic soup components are identified as soup-specific by the non-targeted LCMS approach.
Other components were putatively identified as drugs, which may be a consequence of the skewed nature of the available databases towards pharmaceuticals, but some components may be more relevant. For example, colestipol (m/z 302, C11H28ClN5O) is a bile acid sequestrant often prescribed for ileostomy patients (https://bnf.nice.org.uk/drug/colestipol-hydrochloride.html). Napsagatran (m/z 597; C26H36N6O7S) was putatively identified but it is unknown if this drug was prescribed to the subjects. We can only assume that these drugs have been taken with or before the soups and appear increased due to transit through the gut with the food bolus.
We also attempted to assess the antimicrobial effect of sulforaphane extracted from ileostomy fluid against E. coli K12, however, it was determined that even the broccoli soup modified to increase sulforaphane could not deliver sufficient concentrations to the terminal portion of the ileum to exert antimicrobial effects colonically. However, the higher concentration of sulforaphane in the broccoli soup with added myrosinase would be within the inhibitory range determined, so it is possible that this broccoli soup could inhibit the growth of bacteria present in the stomach and upper small intestine rather than the lower intestine. Also, the GIs present in the ileal fluids (Fig. 3) represent reservoirs of sulforaphane (i.e., from the major component, glucoraphanin, or other isothiocyanates from other GIs) that could be released by the colonic microflora . Bacteria that could be potentially affected by sulforaphane include Helicobacter pylori, a pathogenic bacterium associated with gastric and duodenal ulcers, which has been suggested through human trials to be inhibited by sulforaphane [58,59,60,61].
To conclude, sulforaphane-enriched extracts from beneforté broccoli exerted anti-microbial activity against gut pathogens in vitro and the inclusion of a heat-tolerant source of myrosinase in cooked broccoli can increase intestinal delivery of sulforaphane, albeit to very low levels.
Higdon JV, Delage B, Williams DE, Dashwood RH (2007) Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacol Res 55(3):224–236
Lam TK, Ruczinski I, Helzlsouer KJ, Shugart YY, Caulfield LE, Alberg AJ (2010) Cruciferous vegetable intake and lung cancer risk: a nested case–control study matched on cigarette smoking. Cancer Epidemiol Biomarkers Prev 19(10):2534–2540
Wu QJ, Yang Y, Vogtmann E, Wang J, Han LH, Li HL, Xiang YB (2013) Cruciferous vegetables intake and the risk of colorectal cancer: a meta-analysis of observational studies. Ann Oncol 24(4):1079–1087
Abbaoui B, Lucas CR, Riedl KM, Clinton SK, Mortazavi A (2018) Cruciferous vegetables, isothiocyanates, and bladder cancer prevention. Mol Nutr Food Res 62(18):e1800079. https://doi.org/10.1002/mnfr.201800079
Mukherjee S, Gangopadhyay H, Das DK (2008) Broccoli: a unique vegetable that protects mammalian hearts through the redox cycling of the thioredoxin super-family. J Agri Food Chem 56:609–617
Wu L, Noyan-Ashraf MH, Facci M, Wang R, Paterson PG, Ferrie A, Juurlink JBH (2004) Dietary approach to attenuate oxi- dative stress, hypertension, and inflammation in the cardiovascular system. Proc Nat Acad Sci USA 101:7094–7099
Rodríguez-Cantú LN, Gutiérrez-Uribe JA, Arriola-Vucovich J, Díaz-De La Garza RI, Fahey JW, Serna-Saldivar SO (2011) Broccoli (Brassica oleracea var. italica) sprouts and extracts rich in glucosinolates and isothiocyanates affect cholesterol metabolism and genes involved in lipid homeostasis in hamsters. J Agric Food Chem 59:1095–1103
Nilsson J, Olsson K, Engqvist G, Ekvall J, Olsson M, Nyman M, Åkesson B (2006) Variation in the content of glucosinolates, hydroxycinnamic acids, carotenoids, total antioxidant capacity and low-molecular-weight carbohydrates in Brassica vegetables. J Sci Food Agric 86:528–538
Vale AP, Santos J, Melia N, Peixoto V, Brito NV, Beatriz M, Oliveira PP (2015) Phytochemical composition and antimicrobial properties of four varieties of Brassica oleracea sprouts. Food Contr 55:248–256
Cartea ME, Francisco M, Soengas P, Velasco P (2011) Phenolic compounds in Brassica vegetables. Molecules 16(1):251–280. https://doi.org/10.3390/molecules16010251
Cieślik E, Leszczyńska T, Filipiak-Florkiewicz A, Sikora E, Pisulewski PM (2007) Effects of some technological processes on glucosinolate contents in cruciferous vegetables. Food Chem 105(3):976–981. https://doi.org/10.1016/j.foodchem.2007.04.047
Verkerk R, Schreiner M, Krumbein A, Ciska E, Holst B, Rowland I, De Schrijver R, Hansen M, Gerhäuser C, Mithen R, Dekker M (2009) Glucosinolates in Brassica vegetables: the influence of the food supply chain on intake, bioavailability and human health. Mol Nutr Food Res 53(Suppl 2):S219. https://doi.org/10.1002/mnfr.200800065
Matusheski NV, Swarup R, Juvik JA, Mithen R, Bennett M, Jeffery EH (2006) Epithiospecifier protein from broccoli (Brassica oleracea L. ssp. italica) inhibits formation of the anticancer agent sulforaphane. J Agri Food Chem 54:2069–2076
Juge N, Mithen RF, Traka M (2007) Molecular basis for chemoprevention by sulforaphane: a comprehensive review. Cell Mol Life Sci 64:1105–1127
Amjad AI, Parikh RA, Appleman LJ, Hahm ER, Singh K, Singh SV (2015) Broccoli-derived sulforaphane and chemoprevention of prostate cancer: from bench to bedside. Curr Pharmacol Rep 1:382–390
Liang H, Lai B, Yuan Q (2008) Sulforaphane induces cell-cycle arrest and apoptosis in cultured human lung adenocarcinoma LTEP-A2 cells and retards growth of LTEP-A2 xenografts in vivo. J Nat Prod 71:1911–1914
Parnaud G, Li P, Cassar G, Rouimi P, Tulliez J, Combaret L, Gamet-Payrastre L (2004) Mechanism of sulforaphane-induced cell cycle arrest and apoptosis in human colon cancer cells. Nutr Cancer 48:198–206
Gamet-Payrastre L, Li P, Lumeau S, Cassar G, Dupont MA, Chevolleau S, Gasc N, Tulliez J, Terce F (2000) Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Can Res 60:1426–1433
Aires A, Mota VR, Saavedra MJ, Rosa EA, Bennett RN (2009) The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract. J Appl Microbiol 106:2086–2095
Fahey JW, Haristoy X, Dolan PM, Kensler TW, Scholtus I, Stephenson KK, Talalay P, Lozniewski A (2002) Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of helicobacter pylori and prevents benzo[a]pyrene-induced stomach tumors. Proc Natl Acad Sci 99:7610–7615
Haristoy X, Angioi-duprez K, Duprez A, Lozniewski A (2003) Efficacy of sulforaphane in eradicating helicobacter pylori in human gastric xenografts implanted in nude mice. Antimicrob Agents Chemother 47:3982–3984
Vermeulen M, Klopping-Ketelaars IW, Van Den Berg R, Vses WH (2008) Bioavailability and kinetics of sulforaphane in humans after consumption of cooked versus raw broccoli. J Agric Food Chem 56:10505–10509
Conaway CC, Getahun SM, Liebes LL, Pusateri DJ, Topham DK, Botero-Omary M, Chung FL (2000) Disposition of glucosinolates and sulforaphane in humans after ingestion of steamed and fresh broccoli. Nutr Cancer 38:168–178
Fahey JW, Holtzclaw WD, Wehage SL, Wade KL, Stephenson KK, Talalay P (2015) Sulforaphane bioavailability from glucoraphanin-rich broccoli: control by active endogenous myrosinase. PLoS ONE 10(11):e0140963. https://doi.org/10.1371/journal.pone.0140963
Ghawi SK, Methven L, Niranjan K (2013) The potential to intensify sulforaphane formation in cooked broccoli (Brassica oleracea var. italica) using mustard seeds (Sinapis alba). Food Chem 138:1734–1741
Johnson IT (2002) Glucosinolates: bioavailability and importance to health. Int J Vitam Nutr Res 72:26–31
Petri N, Tannergren C, Holst B et al (2003) Absorption/metabolism of sulforaphane and quercetin, and regulation of phase II enzymes, in human jejunum in vivo. Drug Metab Dispos 31(6):805–813
Traka MH, Saha S, Huseby S, Kopriva S, Walley PG, Barker GC, Moore J, Mero G, van den Bosch F, Constant H et al (2013) Genetic regulation of glucoraphanin accumulation in Beneforté broccoli. New Phytol 198:1085–1095
Gasper AV, Al-Janobi A, Smith JA, Bacon JR, Fortun P, Atherton C, Taylor MA, Hawkey CJ, Barrett DA, Mithen RF (2005) Glutathione S-transferase M1 polymorphism and metabolism of sulforaphane from standard and high-glucosinolate broccoli. Am J Clin Nutr 82(6):1283–1291
Oerlemans K, Barrett DM, Suades CB, Verkerk R, Dekker M (2006) Thermal degradation of glucosinolates in red cabbage. Food Chem 95:19–29
Schneider D, Duperchy E, Depeyrot J, Coursange E, Lenski R, Blot M (2002) Genomic comparisons among Escherichia coli strains B, K-12, and O157:H7 using IS elements as molecular markers. BMC Microbiol 2:18. https://doi.org/10.1186/1471-2180-2-18
Jørgensen F, Leach S, Wilde SJ, Davies A, Stewart GSAB, Humphrey T (2000) Invasiveness in chickens, stress resistance and RpoS status of wild-type Salmonella enterica subsp. enterica serovar Typhimurium definitive type 104 and serovar Enteritidis phage type 4 strains. Microbiology 146:3227–3235. https://doi.org/10.1099/00221287-146-12-3227
Alonzo F, Bobo LD, Skiest DJ, Freitag NE (2011) Evidence for subpopulations of Listeria monocytogenes with enhanced invasion of cardiac cells. J Med Microbiol 60(Pt 4):423–434. https://doi.org/10.1099/jmm.0.027185-0
Feehily C, Finnerty A, Casey PG, Hill C, Gahan CGM et al (2014) Divergent evolution of the activity and regulation of the glutamate decarboxylase systems in listeria monocytogenes EGD-e and 10403S: roles in virulence and acid tolerance. PLoS ONE 9(11):e112649. https://doi.org/10.1371/journal.pone.0112649
Wiegand I, Hilpert K, Hancock REW (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration (mic) of antimicrobial substances. Nat Protoc 3:163–175. https://doi.org/10.1038/nprot.2007.521
McDougall GJ, Allwood JW, Pereira-Caro G, Brown EM, Verrall S, Stewart D, Latimer C, McMullan G, Lawther R, O'Connor G, Rowland I, Crozier A, Gill CI (2017) Novel colon-available triterpenoids identified in raspberry fruits exhibit antigenotoxic activities in vitro. Mol Nutr Food Res 61(2):1600327. https://doi.org/10.1002/mnfr.201600327
McDougall GJ, Allwood JW, Pereira-Caro G, Brown EM, Latimer C, Dobson G, Stewart D, Ternan NG, Lawther R, O'Connor G, Rowland I, Crozier A, Gill CIR (2017) The composition of potentially bioactive triterpenoid glycosides in red raspberry is influenced by tissue, extraction procedure and genotype. Food Funct 8(10):3469–3479
Hagl S, Deusser H, Soyalan B, Janzowski C, Will F, Dietrich H, Albert FW, Rohner S, Richling E (2011) Colonic availability of polyphenols and d-(–)-quinic acid after apple smoothie consumption. Mol Nutr Food Res 55:368–377. https://doi.org/10.1002/mnfr.201000252
Kahle K, Huemmer W, Kempf M, Scheppach W, Erk T, Richling E (2007) Polyphenols are intensively metabolized in the human gastrointestinal tract after apple juice consumption. J Agric Food Chem 55(26):10605–10614. https://doi.org/10.1021/jf071942r
McDougall GJ, Conner S, Pereira-Caro G et al (2014) Tracking (poly)phenol components from raspberries in ileal fluid. J Agric Food Chem 62(30):7631–7641. https://doi.org/10.1021/jf502259j
Brown M, Dunn WB, Dobson P, Patel Y, Winder CL, Francis-McIntyre S, Begley P, Carroll K, Broadhurst D, Tseng A, Swainston N, Spasic I, Goodacre R, Kell DB (2009) Mass spectrometry tools and metabolite-specific databases for molecular identification in metabolomics. Analyst 134:1322–1332
Allwood JW, Weber RJM, Zhou J, He S, Viant MR, Dunn WB (2013) CASMI-the small molecule identification process from a Birmingham perspective. Metabolites 3:397–411
Dai R, Lim L (2014) Release of allyl isothiocyanate from mustard seed meal powder. J Food Sci 79(1):E47–53. https://doi.org/10.1111/1750-3841.12322
Forlin A (2014) Caratterizzazione metabolomica di organismi vegetali naturali e geneticamente modificati. Ph.D thesis, Universita Ca’Foscari Venezia available at https://hdl.handle.net/10579/5396
Ghawi SK, Methven L, Rastall RA, Niranjan K (2012) Thermal and high hydrostatic pressure inactivation of myrosinase from green cabbage: a kinetic study. Food Chem 131(4):1240–1247
Matusheski NV, Jeffery EH (2001) Comparison of the bioactivity of two glucoraphanin hydrolysis products found in broccoli, sulforaphane and sulforaphane nitrile. J Agric Food Chem 49(12):5743–5749
Matusheski NV, Juvik JA, Jeffery EH (2004) Heating decreases epithiospecifier protein activity and increases sulforaphane formation in broccoli. Phytochemistry 65(9):1273–1281
Williams DJ, Critchley C, Pun S, Nottingham S, O’hare TJ (2008) Epithiospecifier protein activity in broccoli: the link between terminal alkenyl glucosinolates and sulphoraphane nitrile. Phytochemistry 69(16):2765–2773
Van Eylen D, Indrawati HM, Van Loey A (2006) Temperature and pressure stability of mustard seed (sinapis alba l.) myrosinase. Food Chem 97:263–271. https://doi.org/10.1016/j.foodchem.2005.03.046
Johansson NL, Pavia CS, Chiao JW (2008) Growth inhibition of a spectrum of bacterial and fungal pathogens by sulforaphane, an isothiocyanate product found in broccoli and other cruciferous vegetables. Planta Med 74:747–750
Aires A, Dias CS, Rosa EA, Saavedra MJ (2013) Antimicrobial susceptibility of Aeromonas spp. isolated from pig ileum segments to natural isothiocyanates. Med Chem 9:861–866
Nowicki D, Rodzik O, Herman-Antosiewicz A, Szalewska-Pałasz A (2016) Isothiocyanates as effective agents against enterohemorrhagic Escherichia coli: insight to the mode of action. Sci Rep 6:22263
Saavedra MJ, Dias CS, Martinez-Murcia A, Bennett RN, Aires A, Rosa EA (2012) Antibacterial effects of glucosinolate-derived hydrolysis products against enterobacteriaceae and enterococci isolated from pig ileum segments. Foodborne Pathog Dis 9(4):338–345
Dean-nystrom EA, Bosworth BT, Cray WC Jr, Moon HW (1997) Pathogenicity of Escherichia coli O157:H7 in the intestines of neonatal calves. Infect Immun 65:1842–1848
Bhan MK, Raj P, Khoshoo V, Bhandari N, Sazawal S, Kumar R, Srivastava R, Arora NK (1989) Quantitation and properties of fecal and upper small intestinal aerobic microflora in infants and young children with persistent diarrhea. J Pediatr Gastroenterol Nutr 9:40–45
Ramaswamy V, Cresence VM, Rejitha JS, Lekshmi MU, Dharsana KS, Prasad SP, Vijila HM (2007) Listeria—review of epidemiology and pathogenesis. J Microbiol Immunol Infect 40:4–13
Okunade O, Niranjan K, Ghawi SK, Kuhnle G, Methven L (2018) Supplementation of the diet by exogenous myrosinase via mustard seeds to increase the bioavailability of sulforaphane in healthy human subjects after the consumption of cooked broccoli. Mol Nutr Food Res 62(18):e1700980
Bahadoran Z, Mirmiran P, Yeganeh MZ, Hosseinpanah F, Zojaji H, Azizi F (2014) Complementary and alternative medicinal effects of broccoli sprouts powder on Helicobacter pylori eradication rate in type 2 diabetic patients: a randomized clinical trial. J Funct Foods 7:390–397
Zhou R, Lin J (1840) Wu D (2014) Sulforaphane induces Nrf2 and protects against CYP2E1 dependent binge alcohol-induced liver steatosis. Biochim Biophys Acta 1:209–218. https://doi.org/10.1016/j.bbagen.2013.09.018
Yanaka A, Fahey JW, Fukumoto A et al (2009) Dietary sulforaphane-rich broccoli sprouts reduce colonization and attenuate gastritis in Helicobacter pylori-infected mice and humans. Cancer Prev Res (Phila) 2(4):353–360
Galan MV, Kishan AA, Silverman AL (2004) Oral broccoli sprouts for the treatment of Helicobacter pylori infection: a preliminary report. Dig Dis Sci 49:1088–1090
This study was supported by Ministry of Higher Education in Libya.
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Abukhabta, S., Khalil Ghawi, S., Karatzas, K.A. et al. Sulforaphane-enriched extracts from glucoraphanin-rich broccoli exert antimicrobial activity against gut pathogens in vitro and innovative cooking methods increase in vivo intestinal delivery of sulforaphane. Eur J Nutr 60, 1263–1276 (2021). https://doi.org/10.1007/s00394-020-02322-0